M Meakin

University of Birmingham, Birmingham, England, United Kingdom

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Publications (3)4.71 Total impact

  • J Stuart · M W Kenny · M Meakin · G S Lucas · N M Caldwell ·
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    ABSTRACT: Positive-pressure and initial-flow-rate (Hémorhéomètre) methods for the study of erythrocyte filtration through 5 micron diameter pores are highly sensitive to the presence of contaminating leucocytes in the erythrocyte test suspension. A pre-filtration step, in which heparinised or EDTA-anticoagulated whole-blood was passed through a column of Imugard IG500 cotton wool, was therefore developed. This procedure removed contaminating platelets and leucocytes, but not erythrocyte sub-populations, and is likely to improve the sensitivity and specificity of erythrocyte filtration techniques.
    Biorheology. Supplement: the official journal of the International Society of Biorheology 02/1984; 1:283-5.
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    ABSTRACT: Positive-pressure and initial-flow-rate (Hémorhéomètre) filtration systems were used to study the deformability of erythrocytes from whole blood stored in EDTA or heparin. When all contaminating platelets and leucocytes were removed from the erythrocyte suspension there was no significant anticoagulant effect on erythrocyte filtration. Blood may therefore be stored in K2EDTA (1.5 mg/ml blood) or lithium heparin (15 IU/ml blood) for up to 6 hours at room temperature prior to measurement of erythrocyte filterability.
    Biorheology. Supplement: the official journal of the International Society of Biorheology 02/1984; 1:279-81.
  • M W Kenny · M Meakin · D J Worthington · J Stuart ·
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    ABSTRACT: A serial study of erythrocyte deformability, plasma viscosity, and whole-blood viscosity has been made during 10 sickle-cell vaso-occlusive crises. The peak serum lactate dehydrogenase level was used to confirm the duration of crisis and the rheological changes were compared with 19 estimations made on the same patients when asymptomatic. Erythrocyte deformability, measured by filtration of washed erythrocytes through polycarbonate filters of 5 microgram pore size, was significantly reduced on day 1 of crisis and, in one additional patient, this occurred 24 h before the onset of pain. There was no increase in irreversibly-sick-led-cell counts and plasma- and blood-viscosity did not increase significantly until day 5 of crisis, in parallel with the acute-phase rise in plasma fibrinogen. Measurement of erythrocyte filterability is therefore a valuable technique for investigating the pathogenesis of the early stages of sickle-cell crisis.
    British Journal of Haematology 10/1981; 49(1):103-9. DOI:10.1111/j.1365-2141.1981.tb07202.x · 4.71 Impact Factor