Hye Soo Lee

Chonbuk National University Hospital, Sŏul, Seoul, South Korea

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Publications (22)33.8 Total impact

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    ABSTRACT: This study examined the serotype distribution and antimicrobial resistance of pneumococcal isolates from invasive infections in children between 2006 and 2010, when the 7-valent pneumococcal conjugate vaccine (PCV7) was offered as an optional vaccine in Korea. Among 140 isolates collected from 8 centers, the common serotypes were 19A (22.9%), 19 F (12.1%), and 6B (8.6%). Between 2006 and 2010, PCV7 serotypes decreased from 62.5% to 21.4% (P = 0.002), whereas 3 PCV13-specific serotypes (3, 6A, and 19A) increased from 18.8% to 42.9% (P = 0.016). Among 102 multidrug-resistant isolates, the proportion of PCV7 serotypes decreased from 65.2% to 21.7% (P = 0.001), and 3 PCV13-specific serotypes increased from 17.4% to 47.8% (P = 0.008). Optional PCV7 vaccination has influenced the proportion of PCV7 serotypes in Korea, resulting in a decrease, whereas the proportions of 3 PCV13-specific serotypes, particularly 19A, have increased.
    Diagnostic microbiology and infectious disease 01/2014; · 2.45 Impact Factor
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    ABSTRACT: We assessed the accuracy of yeast bloodstream isolate identification performed over a 1-year period at 10 Korean hospitals, using the MALDI-TOF-based VITEK MS system. The overall phenotypic misidentification rate was 3.4% (18/533), with considerable variation between hospitals (0.0% to 19.0%), compared to 1.1% (6/533) for the VITEK MS system.
    Journal of clinical microbiology 06/2013; · 4.16 Impact Factor
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    ABSTRACT: At present, the clinical breakpoints (CBPs) of both fluconazole and voriconazole are available only for 3 common Candida species in the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) methods. Epidemiological cutoff values (ECVs) were recently applied to both methods to detect the emergence of acquired resistance (i.e., non-wild-type isolates) among 5 common Candida species. We performed a nationwide study to determine the fluconazole and voriconazole susceptibility of Candida bloodstream isolates (BSIs) using both the CLSI and EUCAST methods. A total of 423 BSIs of 5 Candida species were collected from 8 hospitals. The azole susceptibilities were assessed on the basis of the species-specific CBPs and ECVs. Of the 341 BSIs of 3 common Candida species (i.e., C. albicans, C. tropicalis, and C. parapsilosis), 0.3% and 0.9%, 0.0% and 1.5% of isolates were categorized as fluconazole and voriconazole resistant according to the CLSI and EUCAST CBPs, respectively. Of 423 total BSIs, 1.4% and 2.6% had fluconazole minimum inhibitory concentrations (MICs) exceeding the ECVs according to the CLSI and EUCAST, respectively; 1.0% and 2.1% had voriconazole MICs exceeding the ECVs according to the CLSI and EUCAST, respectively. Categorical agreement between the methods using ECVs was 98.3% for fluconazole and 98.3% for voriconazole. The EUCAST and CLSI methods using ECVs provide highly concordant results. Moreover, non-wild-type isolates with possibly acquired azole resistance were rare among the BSIs of 5 common Candida species in Korea.
    Annals of Laboratory Medicine 05/2013; 33(3):167-73. · 1.48 Impact Factor
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    ABSTRACT: The aim of this study was to determine antimicrobial susceptibility of recent clinical Stenotrophomonas maltophilia isolates from Korea, and to compare the activity levels of several combinations of antimicrobials. A total of 206 non-duplicate clinical isolates of S. maltophilia was collected in 2010 from 11 university hospitals. Antimicrobial susceptibility testing was performed using the Clinical Laboratory Standards Institute agar dilution method. In vitro activity of antimicrobial combinations was tested using the checkerboard method. The susceptibility rates to trimethoprim-sulfamethoxazole and minocycline were 96% and 99%, respectively. The susceptibility rate to levofloxacin was 64%. All of four antimicrobial combinations showed synergy against many S. maltophilia isolates. A combination of trimethoprim-sulfamethoxazole plus ticarcillin-clavulanate was most synergistic among the combinations. None of the combinations showed antagonistic activity. Therefore, some of the combinations may be more useful than individual drugs in the treatment of S. maltophilia infection. Further clinical studies are warranted to validate our in vitro test results.
    Journal of Korean medical science 01/2013; 28(1):62-6. · 0.84 Impact Factor
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    ABSTRACT: Posterior lumbar interbody fusion (PLIF) using harvested local bone inserted into a polyetheretherketone (PEEK) cage is a commonly used procedure, but the accurate fusion rate of a cage, cage to bone contact area ratio, and the changes in fusion rate with time after surgery are unknown. The aim was to conduct a quantitative analysis of the fusion rates and the cage to bone contact area ratios at each period of time using a PEEK cage in PLIF using a consecutive three-dimensional (3D) computed tomography (CT) scan. This was a prospective study using a consecutive 3D thin-section CT scan. Thirty patients aged between 37 and 73 years set to undergo elective PLIF with PEEK cages and pedicle screw fixation were included in the study. The assessments included the Korean Oswestry Disability Index (K-ODI), Short Form (SF)-36 questionnaire, Visual Analog Scale (VAS) pain score, and dynamic plane radiographs, preoperatively and at 6 and 12 months after surgery. Three-dimensional CT scans were performed at 6 and 12 months after surgery. Three-dimensional CT assessments of the following were performed: fusion rate of the cage, cage to bone contact area ratio, and fusion rate of the interbody bone graft besides the cage. The 6-month fusion rate of the segment was 86.7%, which increased to 90.0% at 12 months. The fusion area ratio between the cage area and end plate showed a significant increase from 52.0% at 6 months to 58.5% at 12 months. Regarding the fusion area ratio between the cage and end plate, the ratio between the lower surface of the cage was significantly higher than that of the upper surface. In addition, the K-ODI, SF-36, and VAS values were similar at 6 and 12 months after surgery. The fusion rate of the PEEK cage used in PLIF measured at 12 months was higher than that measured at 6 months. Therefore, an assessment on the complete fusion of local bone at 12 months after surgery is more accurate.
    The spine journal: official journal of the North American Spine Society 05/2011; 11(7):647-53. · 2.90 Impact Factor
  • Mycoses 05/2011; 54(5):e659-62. · 1.28 Impact Factor
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    ABSTRACT: Multilocus sequence typing (MLST) has been successfully applied to the epidemiology of Candida albicans isolates not only within the hospital setting but also in multiple locations nationwide. We performed MLST to investigate the genetic relatedness among bloodstream infection (BSI) isolates of C. albicans recovered from 10 Korean hospitals over a 12-month period. The 156 isolates yielded 112 unique diploid sequence types (DSTs). While 95 DSTs were each derived from a single isolate, 17 DSTs were shared by 61 isolates (39.1%). Interestingly, 111 (71.1%) isolates clustered within previously known clades, and 29 (18.6%) clustered within a new clade that includes strains of Asian origin previously typed as singletons. This MLST study was complemented by restriction endonuclease analysis of genomic DNA using BssHII (REAG-B) in order to evaluate whether strains with identical DSTs and originating from the same hospital corresponded to nosocomial clusters. Importantly, only those isolates with a strong epidemiological relationship showed ≥95% identical REAG-B types. Our results indicate that REAG-B typing can be complementary to MLST but should be limited to the investigation of isolates of identical DSTs and when interhuman transmission is suspected.
    Journal of clinical microbiology 05/2011; 49(7):2572-7. · 4.16 Impact Factor
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    ABSTRACT: Bisphosphonates have been reported to accelerate osteoblastic differentiation of human mesenchymal stem cells, and bioactive glass-ceramics have been used as bone graft extender due to high osteoconductivity. The purpose of this study is to find out what effects CaO-SiO2-P2O5-B2O3 glass-ceramics (BGS-7) treated with alendronate have on the osteoblastic differentiation of human mesenchymal stem cells. Human bone marrow mesenchymal stem cells cultured on BGS-7, hydroxyapatite and cell plate are treated with different concentrations of alendronate and analyzed by MTS assay, ALP assay, RT-PCR, western blot analysis and SEM. The result of the MTS assay shows that the OD value was highest in BGS-7, followed by HA and the plate, and the ALP assay shows that the OD value was higher in the BGS-7 group than the plate group when treated with 10-7 M alendronate. According to the RT-PCR analysis, osteopontin had higher expression in the alendronate-treated group than the non-treated group upon one-week differentiation, showing a clear increase in BGS-7, and osteocalcin and runx-2 showed enhanced expression in the BGS-7 group treated with 10-7Malendronate during week 3 of differentiation. Also, in the western blot analysis, the expression of osteocalcin and runx-2 was increased in the BGS-7 group compared to the plate group in terms of protein expression. The SEM assay shows that the presence of osteoblasts was more evident and the mineral content was higher in the alendronate-treated BGS-7 group than in the alendronate-non treated BGS-7 and alendronate-treated HA. When treated with alendronate, BGS-7, HA and cell plate all accelerated the osteoblastic differentiation of human bone marrow mesenchymal stem cells and especially, the osteoblastic differentiation was accelerated even more in CaO-SiO2-P2O5-B2O3 glass-ceramics (BGS-7) than hydroxyapatite.
    Tissue Engineering and Regenerative Medicine. 01/2011; 8(1):69-77.
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    ABSTRACT: Recombinant human bone morphogenetic proten-2 (rhBMP-2) has an osteoinductivity and it is therefore used as a bone graft substitute. Currently in a clinical setting, mammalian cell origin rhBMP-2 is used. Due to its low yield and a high cost, however, it is disadvantageous in that it cannot be widely used. Given this background, we synthesized E.coli-derived rhBMP-2 and then confirmed the presence of rhBMP-2 using SDS-PAGE and western blot. Besides, using human bone marrow mesenchymal stem cells, alkaline phosphatase (ALP) staining, Alizarin-red staining (AR-S), ALP activity assay and calcium assay were performed for 100 ng/ml or 500 ng/ml rhBMP-2. Furthermore, the RT-PCR was performed for the gene expression of bone sialoprotein (BSP), collagen I and ALP. Besides, using SD-rat abdominal muscle pouch model, an ectopic bone formation was evaluated. SDSPAGE and Western blot results showed that purified rhBMP-2 is the same of reference rhBMP-2. In addition, ALP staining property and AR-S one were of relatively higher degree on days 7 and 21, respectively, in the rhBMP-2 group. Besides, the degree of ALP and calcium assay were also significantly higher in the rhBMP-2. From day 7 on, the mRNA expression of ALP and BSP was of relatively higher degree in the rhBMP-2 group. Rat abdomen ectopic bone formation was confirmed in all the nine samples of the rhBMP-2 group. Based on these results, E.coli-derived rhBMP-2 has osteoblastic differentiation and ectopic bone formation. This led to the confirmation that it could also be used as a bone substitute.
    Tissue Engineering and Regenerative Medicine. 01/2011; 8(1):8-15.
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    ABSTRACT: The Beijing family of Mycobacterium tuberculosis has been emerging in the world. However, there are few nationwide data of genotypic distribution in Korea. This study aimed to identify the genotypic diversity of clinical isolates of M. tuberculosis and to demonstrate the population of Beijing family in Korea. We collected 96 clinical M. tuberculosis isolates from 11 university hospitals nationwide in Korea from 2008 to 2009. We observed 24 clusters in IS6110-RFLP analysis and 19 patterns in spoligotyping. Seventy-five isolates were confirmed to be Beijing family. Two isolates of the K strain and 12 isolates of the K family strain were also found. We found that drug resistance phenotypes were more strongly associated with Beijing family than non-Beijing family (P=0.003). This study gives an overview of the distribution of genotypes of M. tuberculosis in Korea. These findings indicate that we have to pay more attention to control of M. tuberculosis strains associated with the Beijing family.
    Journal of Korean medical science 12/2010; 25(12):1716-21. · 0.84 Impact Factor
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    ABSTRACT: Malignant hyperthermia (MH) is genetically heterogeneous, with mutations in the gene encoding the skeletal muscle ryanodine receptor (RYR1) at 19q13.1 accounting for up to 80% of the cases. However, the search for known and novel mutations in the RYR1 gene is hampered by the fact that the gene contains 106 exons. We aimed to analyze mutations from the entire RYR1 coding region in Korean MH families. We investigated seven affected MH individuals and their family members. The entire RYR1 coding region from the genomic DNA was sequenced, and RYR1 haplotyping and mutational analysis were carried out. We identified nine different RYR1 mutations or variations from seven Korean MH families. Among these, five previously reported mutations (p.Gly248Arg, p.Arg2435His, p.Arg2458His, p.Arg2676Trp, and p.Leu4838Val) and four novel variations of unknown significance (p.Arg2508Cys, p.Met4022Val, p.Glu2669Lys, and p.Ala4295Val) were identified. In two families, two variations (R2676W & M4022V, R2435H & A4295V, respectively) were identified simultaneously. Four of the observed nine mutations or variations were located outside the hotspot region of RYR1 mutations. These data indicate that RYR1 is a main candidate gene in Korean MH families, and that comprehensive screening of the entire coding sequence of the RYR1 gene is necessary for molecular genetic investigations in MH-susceptible individuals, owing to the presence of RYR1 mutations or variations outside of the hotspot region.
    The Korean Journal of Laboratory Medicine 12/2010; 30(6):702-10. · 0.72 Impact Factor
  • International journal of antimicrobial agents 10/2009; 34(6):612-4. · 3.03 Impact Factor
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    ABSTRACT: In smokers, smoking causes many disease entities including cancers, chronic pulmonary diseases and cardiovascular diseases. Passive smoking is also accepted as a carcinogen and its adverse health effects are emphasized. We measured blood vitamin A, C, E (alpha-, beta- and gamma-tocopherol), coenzyme Q10 and urine cotinine concentrations in nonsmokers and smokers. Twenty-one healthy nonsmokers and 24 healthy smokers were included in this study. Smoking status was assessed with a self-reported questionnaire. Plasma was analyzed for coenzyme Q10 and serum for vitamin A, C, E using HPLC (Agilent Technologies Inc., USA) and random urine for cotinine using LC/tandem mass spectrometry (Applied Biosystems Inc., Canada). Smokers had significantly lower serum concentrations of vitamin C than nonsmokers (P=0.0005). No significant differences in concentrations of serum vitamin A, E, and plasma coenzyme Q10 were observed. Smokers had highly elevated urine cotinine levels (1,454+/-903 ng/mL). In 16 (76.2%) of 21 nonsmokers, urine cotinine was detected (3.25+/-4.08 ng/mL). The correlations between urine cotinine and blood antioxidants levels were not found. Neither, the correlation between smoking status and blood antioxidants & urine cotinine was found. This study shows that smokers had significantly lower vitamin C levels among nonenzymatic antioxidants, namely, vitamin A, C, E and coenzyme Q10. High detection rate of urine cotinine in nonsmokers show the seriousness of passive smoking exposure, therefore more social efforts should be directed to reduce passive smoking exposure.
    The Korean Journal of Laboratory Medicine 02/2009; 29(1):10-6. · 0.72 Impact Factor
  • The Korean journal of hematology 01/2009; 44(4).
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    ABSTRACT: There have been very few multicenter studies of the relationship between the use of antifungals and resistance to them. We investigated the antifungal susceptibility of 1,301 clinical isolates of Candida collected from nine Korean hospitals during a 3-month period in 2006 to explore the existence of this type of relationship. Antifungal usage in the preceding year, defined as the daily dose per 1,000 patient days (DDD/1,000 PD), was calculated for each hospital. Resistance to fluconazole, itraconazole, and amphotericin B was detected in 2, 9, and 0.2% of the isolates, respectively. The MIC(50)/MIC(90) values were 0.03/0.125 mg/L for voriconazole, 0.06/0.25 mg/l for caspofungin, and 0.03/0.125 mg/l for micafungin. The total usage of systemic antifungals varied considerably among the nine hospitals, ranging from 6.1 to 96.2 DDD/1,000 PD. No relationship was found between the use of fluconazole (MIC> or =64 mg/l) or itraconazole (MIC> or =1 mg/l) and resistance in the Candida species (P>0.05). However, significant correlations were found between the percentage of Candida isolates that were non-susceptible to fluconazole (MIC> or =16 mg/l) and fluconazole usage (r=0.733, P=0.025) or total antifungal usage (r=0.767, P=0.016).
    Medical mycology: official publication of the International Society for Human and Animal Mycology 07/2008; 47(3):296-304. · 2.13 Impact Factor
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    ABSTRACT: The incidence of Candida bloodstream infections (BSI) has increased over the past two decades. The rank order of occurrence and the susceptibility to antifungals of the various Candida species causing BSI are important factors driving the establishment of empirical treatment protocols; however, very limited multi-institutional data are available on Candida bloodstream isolates in Korea. We investigated the susceptibility to azole antifungals and species distribution of 143 Candida bloodstream isolates recovered from eight university hospitals over a six-month period. Minimal inhibitory concentrations (MICs) of fluconazole, itraconazole, and voriconazole for each isolate were determined by the broth microdilution method of the Clinical and Laboratory Standards Institute (CLSI). The Candida species recovered most frequently from the blood cultures was C. albicans (49%), followed by C. parapsilosis (22%), C. tropicalis (14%), and C. glabrata (11%). The MIC ranges for the Candida isolates were 0.125 to 64 microg/mL for fluconazole, 0.03 to 2 microg/mL for itraconazole, and 0.03 to 1 microg/mL for voriconazole. Overall, resistance to fluconazole was found in only 2% of the Candida isolates (3/143), while the dose-dependent susceptibility was found in 6% (8/143). The resistance and dose-dependent susceptibility of itraconazole were found in 4% (6/143) and 14% (20/143) of the isolates, respectively. All bloodstream isolates were susceptible to voriconazole (MIC, < or = 1 microg/mL). Our findings show that C. albicans is the most common cause of Candida-related BSI, followed by C. parapsilosis, and that the rates of resistance to azole antifungals are still low among bloodstream isolates in Korea.
    Yonsei Medical Journal 10/2007; 48(5):779-86. · 1.31 Impact Factor
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    ABSTRACT: The objective of this study was to investigate if antibody to cyclic citrullinated peptide (anti-CCP) is detected in sera of patients with chronic hepatitis B virus (HBV) infection. Serum anti-CCP and IgA, IgG, and IgM rheumatoid factor (RF) isotypes were measured by enzyme-linked immunosorbent assay on 176 non-arthritic patients with HBV infection. IgA RF, IgG RF, and IgM RF were detectable in 29.5, 21, and 18.8% of the tested sera, respectively, with a total seropositivity rate of 42.7%. Marginally elevated anti-CCP was detected in one patient (0.6%). By regression analysis, there was no statistically significant association between the serum levels of anti-CCP and serum IgA, IgG, or IgM RF (R (2) = 0.033, with respective p values of 0.224, 0.297, and 0.334). In conclusion, anti-CCP was rarely detected in non-arthritic patients with HBV infection in contrast to RF. Thus, testing for anti-CCP may be a useful tool for the diagnosis of rheumatoid arthritis in this population.
    Clinical Rheumatology 08/2007; 26(7):1079-82. · 2.04 Impact Factor
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    ABSTRACT: Oral anticoagulation with warfarin requires routine monitoring of prothrombin time to maintain the international normalized ratio (INR) within the appropriate therapeutic range. Coagu- Chek XS (Roche Diagnositic, Germany) is a portable coagulometer that measures the INR. We evaluated the precision and accuracy of CoaguCheck XS by comparing it with CA-1500 (Sysmex, Japan). We analyzed the CV and the correlation of all INR results measured in 68 samples obtained from patients treated with warfarin and 10 samples from control subjects with no history of anticoagulant therapy with CoaguChek XS and CA-1500. We compared the turn-around time between two instruments and evaluated the differences between the results obtained with venous and capillary blood samples and those obtained with different lots of the test strip. We also evaluated the precision of the two instruments in 5 repeated tests with samples of normal and increased INR. Mean INR values of 5 repeated tests with the same samples were similar. The correlation of INR values between two instruments was excellent (r2=0.97, P=0.001), and the difference in the values between the two instruments was mostly within the 95% limit of agreement, but was shown to increase in direct proportion to INR values. The turn-around time of CoaguChek XS was shorter than that of CA-1500. The differences between venous and capillary blood and between different lots of the test trip were not significant (P>0.05). CoaguChek XS showed a good precision and correlation with CA-1500 with a very short turn-around time. This instrument should be clinically useful in monitoring INR of patients with oral anticoagulation.
    The Korean Journal of Laboratory Medicine 06/2007; 27(3):177-81. · 0.72 Impact Factor
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    ABSTRACT: Immature platelet fraction (IPF) is the percentage of reticulated platelet (RP) of total platelet count. We measured an IPF reference range using XE-2100 blood cell counter with upgraded software (Sysmex, Japan) and evaluated the clinical utility of this parameter for the laboratory diagnosis of thrombocytopenia due to an increase in peripheral platelet destruction. Peripheral blood samples collected into K2EDTA (Beckton Dickinson, USA) were analyzed at Chonbuk National University Hospital. One hundred forty-two samples from apparently healthy adults (all routine full blood count parameters including platelets within the healthy reference range) were used to establish a normal reference range for IPF. The patients were classified into 3 groups including hypoplastic (consisted of 22 patients undergoing chemotherapy with falling platelet counts and 14 with aplastic anemia), cirrhotic (40 with cirrhosis of liver), and idiopathic thrombocytopenic purpura (ITP) (14 with ITP) groups. An IPF reference range in healthy individuals was established as 0.4-5.4%, with a mean of 1.7%. A significant increase in IPF values was found in the ITP patient group. The cut-off value of IPF was 6.1% and its sensitivity and specificity were 92.9%, and 82.9% respectively. Reproducibility was good. A rapid, inexpensive automated method for measuring IPF is feasible and should become a standard parameter in evaluating thrombocytopenic patients.
    The Korean Journal of Laboratory Medicine 03/2007; 27(1):1-6. · 0.72 Impact Factor
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    ABSTRACT: Granulocytic sarcoma of the uterine adnexa is a rare event. A 50-year-old woman, who had previously been diagnosed as chronic myeloid leukemia (CML), but had a complete hematologic response, presented with lower abdominal pain and a large pelvic mass involving the right uterine adnexa region and extending to the right posterior wall of the bladder and right distal ureter. A biopsy of the uterine adnexa revealed granulocytic sarcoma, and a subsequent bone marrow biopsy confirmed the diagnosis of CML in the blastic phase.
    The Korean Journal of Laboratory Medicine 07/2006; 26(3):143-5. · 0.72 Impact Factor

Publication Stats

121 Citations
33.80 Total Impact Points

Institutions

  • 2006–2014
    • Chonbuk National University Hospital
      Sŏul, Seoul, South Korea
  • 2007–2013
    • Chonnam National University
      • Department of Clinical Laboratory Medicine
      Gwangju, Gwangju, South Korea
    • Gyeongsang National University
      • Department of Internal Medicine
      Chinju, South Gyeongsang, South Korea
  • 2004–2013
    • Yonsei University Hospital
      • Department of Internal Medicine
      Sŏul, Seoul, South Korea
  • 2011
    • Seoul National University
      • Department of Orthopaedic Surgery
      Seoul, Seoul, South Korea
    • Chonnam National University Hospital
      Sŏul, Seoul, South Korea