Worapan Pormsila

Universität Basel, Bâle, Basel-City, Switzerland

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Publications (6)21.21 Total impact

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    ABSTRACT: A capillary electrophoresis method with contactless conductivity detection was evaluated as a new approach for quantification of creatine and phosphocreatine in human quadriceps femoris biopsy samples. The running buffers employed consisted of 1 M acetic acid at a pH of 2.3 for the determination of creatine and 50 mM 3-(N-morpholino)propanesulfonic acid/30 mM histidine at a pH of 6.4 for the determination of phosphocreatine in the centrifuged muscle extracts. The limits of detection for creatine and phosphocreatine were found to be 2.5 and 1.0 μM, respectively. Creatine and phosphocreatine were determined in six human muscle biopsy samples and the results were found comparable to those of a standard enzymatic assay. The procedures developed for creatine and phosphocreatine also allow the determination of creatinine as well as adenosine diphosphate and adenosine triphosphate.
    Analytica chimica acta 05/2012; 727:78-82. DOI:10.1016/j.aca.2012.03.055 · 4.52 Impact Factor
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    ABSTRACT: The quantification of plasma lactate and evaluation of the lactate threshold by CE with capacitively coupled contactless conductivity is demonstrated. The only sample preparation needed was deproteinization with a ACN/methanol mixture. A solution of 10 mmol/L 2-morpholinoethanesulfonic acid monohydrate, 10 mmol/L DL-histidine, 70 μmol/L hexadecyltrimethylammonium bromide, pH 6.0 was found suitable as running buffer. Linearity was achieved for the concentration range of 10-1000 μmol/L with a correlation coefficient of 0.9994. The limit of detection (3 S/N) was determined as 3.2 μmol/L. Intra- and inter-day variabilities were less than 7% RSD. The suitability of the method could be demonstrated by analyzing various clinical samples, where the results correlated satisfactorily with those of an established enzymatic method.
    Electrophoresis 04/2011; 32(8):884-9. DOI:10.1002/elps.201000420 · 3.16 Impact Factor
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    ABSTRACT: A capillary electrophoresis method with contactless conductivity detection was developed for the quantification of carnitine and six acylcarnitines in plasma and urine samples. The running buffer employed consisted of 500 mmol/L acetic acid, 1.0 mmol/L hydroxypropyl-β-cyclodextrin and 0.05% Tween at a pH of 2.6. Under these conditions, the isomeric valproyl- and octanoyl-carnitines could be distinguished. The linearity was in the range from 5.0 to 200.0 μmol/L with correlation coefficients between 0.9992 and 0.9997. The limits of detection were between 1.0 and 3.2 μmol/L. Intra- and inter-day precisions as %RSD were better than 10%. The method allows for direct determination without derivatisation or extraction processes. The method was applied for the quantification of carnitine and acetylcarnitine in plasma pre- and post-exercise, and to measure valproylcarnitine in plasma and urine of patients undergoing valproate therapy.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 03/2011; 879(13-14):921-6. DOI:10.1016/j.jchromb.2011.02.046 · 2.69 Impact Factor
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    ABSTRACT: A CE method with capacitively coupled contactless conductivity detection was developed and tested for the quantification of carnitine in different types of foodstuffs, namely fruit juices, milk, yogurt, cheese, red meat and chicken meat. Sample preparation was minimal as chemical or enzymatic conversion of the analyte is not necessary with the non-UV-absorbing compound when conductivity detection is employed. A 500 mmol/L acetic acid solution at pH 2.6 with 0.05% Tween-20, was used as the optimized running buffer. The analysis time was approximately 4 min. Linearity was achieved for the concentration range of 5-500 micromol/L with a correlation coefficient of 0.9996. The LOD (3 signal/noise) was determined as 2.6 micromol/L. Intra- and inter-day variabilities were less than 10% for both migration time and peak area, indicating a good precision of the method.
    Electrophoresis 07/2010; 31(13):2186-91. DOI:10.1002/elps.200900692 · 3.16 Impact Factor
  • Worapan Pormsila, Xiao Yang Gong, Peter C Hauser
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    ABSTRACT: The enantiomers of the anions of five alpha-hydroxy acids, namely lactic acid, alpha-hydroxybutyric acid, 2-hydroxycaproic acid, 2-hydroxyoctanoic acid and 2-hydroxydecanoic acid, as well as the two alpha-amino acids aspartic acid and glutamic acid, were baseline separated and detected by CE with contactless conductivity detection. Vancomycin was employed as chiral selector and could be used with conductivity detection without having to resort to a partial filling protocol as needed when this reagent is used with UV absorbance measurements. The procedure was successfully applied to the determination of the lactic acid enantiomers in samples of milk and yogurt. Linearity was achieved in the concentration range of 10-500 micromol/L with good correlation coefficients (0.9993 and 0.9990 for L- and D-lactic acid, respectively). The LODs (3 S/N) for L- and D-lactic acid were determined as 2.8 and 2.4 micromol/L, respectively.
    Electrophoresis 06/2010; 31(12):2044-8. DOI:10.1002/elps.201000127 · 3.16 Impact Factor
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    ABSTRACT: The suitability of capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C(4)D) for the direct determination of uric acid in human plasma and urine was investigated. It was found that a careful optimization of the buffer composition and pH was necessary to achieve selective determination in the complex sample matrices. An electrolyte solution consisting of 10mM 2-morpholinoethanesulfonic acid (MES), 10mM histidine and 0.1mM hexadecyltrimethylammonium bromide (CTAB), pH 6.0, was finally found suitable for use as running buffer for both sample matrices. The limit of detection (3 S/N) was determined as 3.3 microM. The linearity of the response was tested for the range between 10 and 500 microM and a correlation coefficient of 0.9996 was obtained. Intra- and inter-day variabilities were <10%. Quantitative analysis of urine and plasma samples showed a good correlation with the routine enzymatic method currently used at the University Hospital of Basel.
    Analytica chimica acta 03/2009; 636(2):224-8. DOI:10.1016/j.aca.2009.02.012 · 4.52 Impact Factor

Publication Stats

87 Citations
21.21 Total Impact Points

Institutions

  • 2009–2011
    • Universität Basel
      • Department of Chemistry
      Bâle, Basel-City, Switzerland