Eun Kyoung Kim

CHA University, Sŏul, Seoul, South Korea

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Publications (86)291.95 Total impact

  • Young-Jin Park · Eun Kyoung Kim · Jung Yoon Bae · Sook Moon · Jin Kim ·
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    ABSTRACT: Human telomerase reverse transcriptase (hTERT) contributes to tumor progression as well as maintaining telomere length, however, the mechanism by which hTERT promotes invasiveness is not yet completely understood. This study aims to unravel the precise mechanism through which hTERT promotes cancer invasion. We established an hTERT-overexpressed immortalized cell line (IHOK/hTERT). In orthotopic xenograft models, IHOK/hTERT harbors higher tumorigenicity than IHOK/Control. IHOK/hTERT showed much higher migration and invasion activities compared to IHOK/Control. IHOK/hTERT co-cultured with fibroblasts displayed increased invasion compared to IHOK/hTERT without fibroblasts. We screened for genes that play an important role in intermodulation between cancer cells and fibroblasts using a microarray and identified fibroblast activation protein (FAP). hTERT knockdown showed decreased expression of FAP and early growth response (EGR)-1, one of the transcriptional regulators of FAP in IHOK/hTERT and oral cancer cell line YD10B. Furthermore, EGR-1 knockdown in IHOK/hTERT and YD10B showed reduced invasion and reduced cathepsin D expression compared to Control-siRNA cells. Taken together, this study provides evidence that hTERT overexpression is responsible for the upregulation of the cysteine protease cathepsin D by regulating EGR-1 to activate invasiveness in cancer progression.
    Cancer letters 10/2015; DOI:10.1016/j.canlet.2015.10.021 · 5.62 Impact Factor

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    ABSTRACT: Hepatocellular carcinoma (HCC) is the fifth most common cancer in Korea. Diverse paraneoplastic syndromes can occur in patients with HCC, but parathyroid hormone-related peptide (PTH-rP)-induced hypercalcemia is uncommon. Hypercalcemia due to PTH or particularly PTH-rP-secreting HCC is associated with poor outcomes. We report a 71-year-old man who presented with symptoms of vague abdominal discomfort, somnolence, lethargy, nausea, vomiting, and weight loss. Imaging studies revealed a large HCC without metastasis. The laboratory findings showed elevated serum calcium level, low intact parathyroid hormone (iPTH) level and elevated PTH-rP level. These results led to a diagnosis of a PTH-rP-secreting HCC and paraneoplastic hypercal-cemia. After emergency management of the hypercalcemia, the patient underwent an extended right hemihepatectomy with cholecystectomy. One year after the surgery, he is alive with normal calcium, PTH-rP, and iPTH levels. This case demonstrates that the rare phenomenon of life-threatening hypercalcemia caused by HCC should not be overlooked. These symptoms offer a good opportunity to diagnose HCC early. Radical tumor resection makes it possible to cure patients with PTH-rP-secreting HCC.
    The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi 08/2015; 66(2):122-6. DOI:10.4166/kjg.2015.66.2.122
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    ABSTRACT: Apical hypertrophic cardiomyopathy (ApHCM) is thought to have a favourable clinical outcome, compared with other types of HCM. We sought to investigate the clinical and anatomical differences in cardiovascular imaging between ApHCM and non-ApHCM. A total of 350 patients diagnosed with HCM underwent cardiovascular magnetic resonance (CMR) and echocardiography. All enrolled subjects were prospectively followed up for adverse clinical outcomes. Eighty-five patients were classified as ApHCM. On CMR, the amount and proportion of late gadolinium enhancement (LGE) as well as left ventricular volumetric parameters were evaluated. Echocardiographic evaluations included diastolic function and global strain. Patients with ApHCM were less likely to present with history of syncope and have less frequency of family history of sudden cardiac death than those with non-ApHCM. Functional class was also more favourable in ApHCM [frequency of New York Heart Association (NYHA) class I; 89.4 vs. 66.8%, P < 0.001]. LGE was less frequently detected (87.1 vs. 93.9%, P = 0.04), and the amount of LGE was significantly smaller in ApHCM (7.0 ± 6.0 vs. 14.6 ± 10.5%, P < 0.001). The E/e' level and left atrial volume index were also lower in ApHCM patients (all P < 0.001). During follow-up, a composite of adverse clinical events including cardiac death, admission for heart failure, and cerebrovascular accident was higher in patients with ApHCM than those with non-ApHCM (P = 0.01). ApHCM showed a relatively small burden of myocardial fibrosis and less severe diastolic dysfunction and subsequently more favourable clinical manifestations in comparison with other HCMs. This may be one explanation of why most patients with ApHCM show a benign course of disease compared with non-ApHCM. Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2015. For permissions please email:
    European Heart Journal Cardiovascular Imaging 08/2015; DOI:10.1093/ehjci/jev192 · 4.11 Impact Factor

  • 08/2015; 89(2):220-224. DOI:10.3904/kjm.2015.89.2.220
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    Eun Kyoung Kim · Pairoj Chattranukulchai · Igor Klem ·
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    ABSTRACT: In patients with non-ischemic cardiomyopathy (NICM), risk stratification for sudden cardiac death (SCD) and selection of patients who would benefit from prophylactic implantable cardioverter-defibrillators remains challenging. We aim to discuss the evidence of cardiac magnetic resonance (CMR)-derived myocardial scar for the prediction of adverse cardiovascular outcomes in NICM. From the 15 studies analyzed, with a total of 2747 patients, the average prevalence of myocardial scar was 41%. In patients with myocardial scar, the risk for adverse cardiac events was more than 3-fold higher, and risk for arrhythmic events 5-fold higher, as compared to patients without scar. Based on the available observational, single center studies, CMR scar assessment may be a promising new tool for SCD risk stratification, which merits further investigation.
    Korean journal of radiology: official journal of the Korean Radiological Society 07/2015; 16(4):683-95. DOI:10.3348/kjr.2015.16.4.683 · 1.57 Impact Factor
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    ABSTRACT: Androgen receptor (AR) signaling is important for prostate cancer (PCa) cell proliferation. Here, we showed that proliferation of hormone-sensitive prostate cancer cells such as LNCaP was significantly enhanced by testosterone stimulation whereas hormone-insensitive prostate cancer cells such as PC3 and VCaP did not respond to testosterone stimulation. Blocking of AR using bicalutamide abolished testosterone-induced proliferation of LNCaP cells. In addition, knockdown of AR blocked testosterone-induced proliferation of LNCaP cells. Basal expression of low-density lipoprotein receptor-related protein 6 (LRP6) was elevated in VCaP cells whereas stimulation of testosterone did not affect the expression of LRP6. However, expression of LRP6 in LNCaP cells was increased by testosterone stimulation. In addition, knockdown of LRP6 abrogated testosterone-induced proliferation of LNCaP cells. Given these results, we suggest that androgen-dependent expression of LRP6 plays a crucial role in hormone-sensitive prostate cancer cell proliferation.
    Korean Journal of Physiology and Pharmacology 05/2015; 19(3):235-40. DOI:10.4196/kjpp.2015.19.3.235 · 1.38 Impact Factor
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    ABSTRACT: Despite technical simplicity and the low cost of brachial-ankle pulse wave velocity (BA-PWV), its use has been hampered by a lack of data supporting its usefulness and reliability. The aim of this study was to evaluate the usefulness of BA-PWV to measure aortic stiffness in comparison to using cardiovascular magnetic resonance (CMR). A total of 124 participants without cardiovascular risk factors volunteered for this study. BA-PWV was measured using a vascular testing device. On the same day, using CMR, cross-sectional areas for distensibility and average blood flow were measured at four aortic levels: the ascending, upper thoracic descending, lower thoracic descending, and abdominal aorta. Compared to PWV measured by CMR, BA-PWV values were significantly higher and the differences therein were similar in all age groups (all p<0.001). There was a significant correlation between BA-PWV and PWV by CMR (r=0.697, p<0.001). Both BA-PWV and PWV by CMR were significantly and positively associated with age (r=0.652 and 0.724, p<0.001). The reciprocal of aortic distensibility also demonstrated a statistically significant positive correlation with BA-PWV (r=0.583 to 0.673, all p<0.001). BA-PWV was well correlated with central aortic PWV and distensibility, as measured by CMR, regardless of age and sex.
    Yonsei medical journal 05/2015; 56(3):617-24. DOI:10.3349/ymj.2015.56.3.617 · 1.29 Impact Factor
  • Song Yoon Baik · Eun Kyoung Kim · Sung Kwan Hong ·

    Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi 04/2015; 48(2):S75-S76. DOI:10.1016/j.jmii.2015.02.267 · 2.35 Impact Factor
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    Farman Ali · Eun Kyoung Kim · Yong-Gi Kim ·
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    ABSTRACT: The volume of traveling websites is rapidly increasing. This makes relevant information extraction more challenging. Several fuzzy ontology-based systems have been proposed to decrease the manual work of a full-text query search engine and opinion mining. However, most search engines are keyword-based, and available full-text search engine systems are still imperfect at extracting precise information using different types of user queries. In opinion mining, travelers do not declare their hotel opinions entirely but express individual feature opinions in reviews. Hotel reviews have numerous uncertainties, and most featured opinions are based on complex linguistic wording (small, big, very good and very bad). Available ontology-based systems cannot extract blurred information from reviews to provide better solutions. To solve these problems, this paper proposes a new extraction and opinion mining system based on a type-2 fuzzy ontology called T2FOBOMIE. The system reformulates the user’s full-text query to extract the user requirement and convert it into the format of a proper classical full-text search engine query. The proposed system retrieves targeted hotel reviews and extracts feature opinions from reviews using a fuzzy domain ontology. The fuzzy domain ontology, user information and hotel information are integrated to form a type-2 fuzzy merged ontology for the retrieving of feature polarity and individual hotel polarity. The Protégé OWL-2 (Ontology Web Language) tool is used to develop the type-2 fuzzy ontology. A series of experiments were designed and demonstrated that T2FOBOMIE performance is highly productive for analyzing reviews and accurate opinion mining.
    Applied Intelligence 04/2015; 42(3). DOI:10.1007/s10489-014-0609-y · 1.85 Impact Factor
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    ABSTRACT: It is uncertain that atorvastatin pretreatment can reduce myocardial damage in patients undergoing primary percutaneous coronary intervention (PCI) for ST-segment elevation myocardial infarction (STEMI). The aim of this study was to investigate the effects of atorvastatin pretreatment on infarct size measured by contrast-enhanced magnetic resonance imaging (CE-MRI) in STEMI patients. Patients undergoing primary PCI for STEMI within 12 hr after symptom onset were randomized to an atorvastatin group (n=30, 80 mg before PCI and for 5 days after PCI) or a control group (n=37, 10 mg daily after PCI). The primary end point was infarct size evaluated as the volume of delayed hyperenhancement by CE-MRI within 14 days after the index event. The median infarct size was 19% (IQR 11.1%-31.4%) in the atorvastatin group vs. 16.3% (7.2%-27.2%) in the control group (P=0.27). The myocardial salvage index (37.1% [26.9%-58.7%] vs. 46.9% [39.9-52.4], P=0.46) and area of microvascular obstruction (1.1% [0%-2.0%] vs. 0.7% [0%-1.8%], P=0.37) did not differ significantly between the groups. Frequency of the hemorrhagic and transmural infarctions was not significantly different in the 2 groups. Pretreatment with a high-dose atorvastatin followed by further treatment for 5 days in STEMI patients undergoing primary PCI failed to reduce the extent of myocardial damage or improve myocardial salvage. Graphical Abstract
    Journal of Korean medical science 04/2015; 30(4):435-41. DOI:10.3346/jkms.2015.30.4.435 · 1.27 Impact Factor
  • Min Kyeong Joo · Eun Kyoung Kim · Sung Kwan Hong ·

    Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi 04/2015; 48(2):S115. DOI:10.1016/j.jmii.2015.02.407 · 2.35 Impact Factor
  • Do Kyeong Kim · Eun Kyoung Kim · Jung Yoon Bae · Jin Kim ·

    03/2015; 119(3):e141-e142. DOI:10.1016/j.oooo.2014.07.170

  • Journal of the American College of Cardiology 03/2015; 65(10):A1269. DOI:10.1016/S0735-1097(15)61269-4 · 16.50 Impact Factor
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    ABSTRACT: In the Effects of Postconditioning on Myocardial Reperfusion in Patients with ST-segment Elevation Myocardial Infarction (POST) trial, ischemic postconditioning failed to improve myocardial reperfusion. However, long-term effects of ischemic postconditioning on clinical outcomes are not known in patients with ST-segment elevation myocardial infarction. A total of 700 patients undergoing primary percutaneous coronary intervention (PCI) were randomly assigned to the postconditioning group or the conventional primary PCI group in a 1:1 ratio. Postconditioning was performed immediately after restoration of coronary flow by balloon occlusion 4 times for 1 minute. Complete follow-up data for major clinical events at 1 year were available in 695 patients (99.3%), and analyses were done by the intention to treat principle. The primary outcome was a composite of death, myocardial infarction, severe heart failure, or stent thrombosis at 1 year. At 1 year, a composite of death, myocardial infarction, severe heart failure, or stent thrombosis occurred in 21 patients (6.1%) in the postconditioning group and 16 patients (4.6%) in the conventional PCI group (hazard ratio [HR] 1.32, 95% CI 0.69-2.53, P = .40). The risk of death (4.9% vs 3.7%, HR 1.32, 95% CI 0.64-2.71, P = .46), heart failure (2.6% vs 2.3%, HR 1.13, 95% CI 0.44-2.94, P = .80), and stent thrombosis (2.3% vs 1.7%, HR 1.34, 95% CI 0.46-3.85, P = .59) did not differ significantly between the 2 groups. Ischemic postconditioning does not seem to improve the 1-year clinical outcomes in patients with ST-segment elevation myocardial infarction undergoing primary PCI. Copyright © 2015 Elsevier Inc. All rights reserved.
    American Heart Journal 02/2015; 169(5). DOI:10.1016/j.ahj.2015.01.015 · 4.46 Impact Factor
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    Journal of Cardiovascular Magnetic Resonance 02/2015; 17(1). DOI:10.1186/1532-429X-17-S1-P278 · 4.56 Impact Factor
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    ABSTRACT: Singleton-Merten syndrome (SMS) is an autosomal-dominant multi-system disorder characterized by dental dysplasia, aortic calcification, skeletal abnormalities, glaucoma, psoriasis, and other conditions. Despite an apparent autosomal-dominant pattern of inheritance, the genetic background of SMS and information about its phenotypic heterogeneity remain unknown. Recently, we found a family affected by glaucoma, aortic calcification, and skeletal abnormalities. Unlike subjects with classic SMS, affected individuals showed normal dentition, suggesting atypical SMS. To identify genetic causes of the disease, we performed exome sequencing in this family and identified a variant (c.1118A>C [p.Glu373Ala]) of DDX58, whose protein product is also known as RIG-I. Further analysis of DDX58 in 100 individuals with congenital glaucoma identified another variant (c.803G>T [p.Cys268Phe]) in a family who harbored neither dental anomalies nor aortic calcification but who suffered from glaucoma and skeletal abnormalities. Cys268 and Glu373 residues of DDX58 belong to ATP-binding motifs I and II, respectively, and these residues are predicted to be located closer to the ADP and RNA molecules than other nonpathogenic missense variants by protein structure analysis. Functional assays revealed that DDX58 alterations confer constitutive activation and thus lead to increased interferon (IFN) activity and IFN-stimulated gene expression. In addition, when we transduced primary human trabecular meshwork cells with c.803G>T (p.Cys268Phe) and c.1118A>C (p.Glu373Ala) mutants, cytopathic effects and a significant decrease in cell number were observed. Taken together, our results demonstrate that DDX58 mutations cause atypical SMS manifesting with variable expression of glaucoma, aortic calcification, and skeletal abnormalities without dental anomalies. Copyright © 2015 The American Society of Human Genetics. Published by Elsevier Inc. All rights reserved.
    The American Journal of Human Genetics 01/2015; 96(2). DOI:10.1016/j.ajhg.2014.11.019 · 10.93 Impact Factor
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    ABSTRACT: In the effects of postconditioning on myocardial reperfusion in patients with ST-segment elevation myocardial infarction (POST) trial, ischemic postconditioning did not improve myocardial reperfusion in 700 patients with STEMI undergoing primary PCI. However, the impact of postconditioning on myocardial salvage and infarct size still needs to be addressed. The aim of this study was to investigate the effect of ischemic postconditioning on myocardial salvage using cardiac magnetic resonance (CMR) in patients with STEMI undergoing primary PCI. For the CMR substudy, a total of 111 patients was analyzed, 56 in the postconditioning group and 55 undergoing conventional primary PCI in the control group. Postconditioning was performed immediately after restoration of coronary flow by four cycles of 1-min balloon occlusion separated by 1 min of deflation. The primary end point was myocardial salvage measured by CMR 3 days after the index event. The myocardial salvage index was not improved by ischemic postconditioning compared with conventional PCI (46.3 ± 18.5 vs. 45.7 ± 20.5 %, p = 0.86). The infarct size was not significantly different between the two groups (18.8 ± 10.3 vs. 20.2 ± 11.0 %, p = 0.52). Moreover, there was no significant difference in the rates of microvascular obstruction or hemorrhagic infarction between the groups. CMR study demonstrated that ischemic postconditioning during primary PCI in STEMI patients did not improve myocardial salvage or reduce infarct size. These findings further support the results of the POST trial which showed no benefit of ischemic postconditioning as an adjunctive treatment of primary PCI.
    The International Journal of Cardiovascular Imaging 01/2015; 31(3). DOI:10.1007/s10554-015-0589-y · 1.81 Impact Factor
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    ABSTRACT: mTORC1 plays a key role in autophagy as a negative regulator. The currently known targets of mTORC1 in the autophagy pathway mainly function at early stages of autophagosome formation. Here, we identify that mTORC1 inhibits later stages of autophagy by phosphorylating UVRAG. Under nutrient-enriched conditions, mTORC1 binds and phosphorylates UVRAG. The phosphorylation positively regulates the association of UVRAG with RUBICON, thereby enhancing the antagonizing effect of RUBICON on UVRAG-mediated autophagosome maturation. Upon dephosphorylation, UVRAG is released from RUBICON to interact with the HOPS complex, a component for the late endosome and lysosome fusion machinery, and enhances autophagosome and endosome maturation. Consequently, the dephosphorylation of UVRAG facilitates the lysosomal degradation of epidermal growth factor receptor (EGFR), reduces EGFR signaling, and suppresses cancer cell proliferation and tumor growth. These results demonstrate that mTORC1 engages in late stages of autophagy and endosome maturation, defining a broader range of mTORC1 functions in the membrane-associated processes. Copyright © 2015 Elsevier Inc. All rights reserved.
    Molecular Cell 12/2014; 57(2). DOI:10.1016/j.molcel.2014.11.013 · 14.02 Impact Factor
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    ABSTRACT: Crosstalk between cancer cells and carcinoma-associated fibroblasts (CAFs) has earned recognition as an interaction that plays a pivotal role in carcinogenesis. Thus, we attempted to clarify whether increase in the level of CAFs promotes cancer progression by proportionally enhancing the interaction between cancer cells and CAFs. We first analyzed clinical correlation between the levels of fibroblasts and cancer progression and found that the level of CAFs made a noticeable difference on the prognosis of patients with oral squamous cell carcinoma (OSCC). In vivo animal study also demonstrated that tumor volume depended on the dose of CAFs that was co-injected with OSCC cells. The same tendency was observed in an in vitro study. We also found that interleukin-1α (IL-1α) secreted from OSCC cells had dual effects on CAFs: IL-1α not only promoted the proliferation of CAFs but also upregulated the secretion of cytokines in CAFs such as CCL7, CXCL1, and IL-8. The induction activity of cytokine secretion by IL-1α surpassed that of proliferation in OSCC cells. In summary, we unraveled an important interactive mechanism of carcinogenesis: IL-1α released from carcinoma stimulates the proliferation of CAFs and the simultaneous increase in cytokine secretion from CAFs promotes cancer progression in human OSCC. On the basis of these findings, we propose that the level of CAFs is eligible for being selected as a prognostic factor that will be useful in routine diagnosis. We also propose that blockage of reciprocal interaction between cancer cells and CAFs will provide an insight for developing novel chemotherapeutic strategy.
    Neoplasia (New York, N.Y.) 11/2014; 16(11). DOI:10.1016/j.neo.2014.09.003 · 4.25 Impact Factor

Publication Stats

485 Citations
291.95 Total Impact Points


  • 2015
    • CHA University
      • Department of Internal Medicine
      Sŏul, Seoul, South Korea
  • 2014-2015
    • Yonsei University
      • Department of Oral Pathology
      Sŏul, Seoul, South Korea
    • Gyeongsang National University
      • Department of Computer Science
      Shinshū, Gyeongsangnam-do, South Korea
    • Wonkwang University
      Riri, North Jeolla, South Korea
  • 2012-2015
    • National Cancer Center Korea
      • Specific Organs Cancer Branch
      QYK, Gyeonggi-do, South Korea
    • Samsung Medical Center
      • Department of Cardiology
      Sŏul, Seoul, South Korea
  • 2007-2015
    • Sungkyunkwan University
      • School of Medicine
      Sŏul, Seoul, South Korea
  • 2008-2014
    • Pusan National University
      • • Department of Pharmacology
      • • Medical Research Center for Ischemic Tissue Regeneration
      Tsau-liang-hai, Busan, South Korea
    • Kyung Hee University
      • College of Oriental Medicine
      Sŏul, Seoul, South Korea
  • 2010-2013
    • Catholic University of Korea
      • • Department of Ophthalmology
      • • College of Medicine
      Sŏul, Seoul, South Korea
    • Korea University
      • Department of Psychology
      Sŏul, Seoul, South Korea
    • Chonbuk National University
      Tsiuentcheou, Jeollabuk-do, South Korea
    • Asan Medical Center
      • Department of Oncology
      Sŏul, Seoul, South Korea
  • 2009-2012
    • Ulsan University Hospital
      Urusan, Ulsan, South Korea
  • 2011
    • Hanyang University Medical Center
      Sŏul, Seoul, South Korea
    • Jeju National University
      • Faculty of Biotechnology
      Tse-tsiu, Jeju, South Korea
    • Ajou University
      • Department of Endocrinology and Metabolism
      Sŏul, Seoul, South Korea
    • Korea Research Institute of Bioscience and Biotechnology KRIBB
      Anzan, Gyeonggi-do, South Korea