In Bum Song

Publications (3)9.03 Total impact

• Source

  Available from: Gilson Khang

  Article: Porcine small intestinal submucosa sheets as a scaffold for human bone marrow stem cells.

  Hyun Hee Ahn, Kyung Sook Kim, Jung Hwa Lee, Min Suk Lee, In Bum Song, Mi Hee Cho, Yu Na Shin, Moon Suk Kim, Gilson Khang, Hai Bang Lee
  [show abstract] [hide abstract]
  ABSTRACT: Native small intestinal submucosa (SIS) sheet was prepared by removal of inside and outside layer of porcine jejunum. The acid treated SIS sheet was also prepared by dipping of native SIS sheet in acetic acid solution. The native or acid treated SIS sheets exhibited elastic and soft property on touch. The surface of native SIS sheet appears to be covered with thin and long collagen fibers entangled into networks. The fibers and fibrils at acid treated SIS sheet disappeared due to the acidic erosion of collagen fiber. The water uptake of acid treated SIS sheet (1300%) was higher than that of the native SIS sheet (500%). The cell morphology and proliferation of human bone marrow stem cells (hBMSCs) on SIS sheet was examined. The hBMSCs on the SIS sheet showed a flattened morphology, while cells in the polyglycolic acid (PGA) mesh showed rounded cell morphology. The cell viability on native or acid treated SIS sheet was higher than that of PGA mesh. The hBMSCs in both native and acid treated SIS sheet were grown at a similar rate. The number of adhering hBMSCs increased with incubation time. Thus, we could confirm that native or acid treated SIS sheet could act as a potential scaffold to enhance the hBMSCs proliferation by providing probably natural environments.
  International Journal of Biological Macromolecules 01/2008; 41(5):590-6. · 2.45 Impact Factor
• Source

  Available from: chonbuk.ac.kr

  Article: In vitro and in vivo release of albumin using a biodegradable MPEG-PCL diblock copolymer as an in situ gel-forming carrier.

  Hoon Hyun, Yu Han Kim, In Bum Song, Jung Won Lee, Moon Suk Kim, Gilson Khang, Kinam Park, Hai Bang Lee
  [show abstract] [hide abstract]
  ABSTRACT: An MPEG-PCL diblock copolymer was synthesized as an in situ gel carrier, and its phase transition behavior in aqueous solutions was examined. For comparison, aqueous solutions of Pluronic F-127, a widely used injectable gel-forming solution, were also studied. Both MPEG-PCL copolymer and Pluronic aqueous solutions were sols at room temperature. As the temperature was increased above room temperature, the diblock copolymer and Pluronic solutions underwent a sol-to-gel phase transition, which manifested as an increase in viscosity indicative of the formation of a gel. All of the copolymer solutions became gels at body temperature, although the gel viscosity increased with the increasing concentration of the MPEG-PCL diblock copolymer in the solution. In in vitro experiments, in which the gels were exposed to PBS, the MPEG-PCL gels maintained their structural integrity for more than 28 days, whereas the Pluronic gel disappeared within 2 days. The same results were observed when the polymer solutions were subcutaneously injected into rats. The MPEG-PCL gels maintained their structural integrity longer than 30 days, while the Pluronic gel could not be observed after 2 days. The ability of the gels as drug carriers was studied by measuring the release of fluorescein isothiocyanate-labeled bovine serum albumin (BSA-FITC) from MPEG-PCL diblock copolymer gels in vitro as well as in vivo. In vitro, BSA release was sustained above 20 days, with a greater release at lower diblock copolymer concentration; by contrast, Pluronic gels exhibited almost complete release of BSA-FITC within 1 day. When the BSA-FITC-loaded diblock copolymer and Pluronic solutions were subcutaneously injected into rats, they immediately transformed into a gel. In vivo, sustained release of BSA-FITC over 30 days was observed from the MPEG-PCL gel, whereas BSA-FITC release from the Pluronic gel ceased within 3 days. Collectively, the present findings show that MPEG-PCL diblock copolymer solutions are thermo-responsive and maintain their structural integrity under physiological conditions, indicating that they are suitable for use as injectable drug carriers.
  Biomacromolecules 05/2007; 8(4):1093-100. · 5.48 Impact Factor
• Article: Preparation of sponge using porcine small intesinal submucosa and their applications as a scaffold and a wound dressing.

  Moon Suk Kim, Min Suk Lee, In Bum Song, Sang Jin Lee, Hai Bang Lee, Gilson Khang, Il Woo Lee
  Advances in experimental medicine and biology 02/2006; 585:209-22. · 1.09 Impact Factor