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Zichun Xiang,
Richard Gonzalez,
Lili Ren,
Yan Xiao,
Lan Chen,
Jing Zhang,
Wei Wang, Qingqing Yang,
Jianguo Li,
Hongli Zhou,
Guy Vernet,
Gláucia Paranhos-Baccalà,
Zhong Wang,
Jianwei Wang
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ABSTRACT: Respiratory syncytial virus (RSV) is a leading cause of respiratory tract illnesses worldwide. Although the prevalence and clinical manifestations of the two subtypes, RSV-A and RSV-B, have been studied in some detail in infants and young children, they have not been determined in adults. To evaluate the prevalence of the RSV subtypes and disease severity between RSV-A and RSV-B infections in adults, nasal and throat swabs that were collected from patients ≥15 years old who sought medical care for acute respiratory infections at the Fever Clinic of the Peking Union Medical College Hospital in Beijing, China between May 2005 and April 2010. The samples were tested for RSV infection using PCR and sequencing analysis. RSV was detected in 95 (1%) of the adult patients, of whom 53 (55.8%) were positive for RSV-A and 42 (44.2%) for RSV-B. The incidence of RSV infections increased with age (χ(2) = 37.17, P = 1.66E-07). Demographic data and clinical manifestations of RSV-A were similar to those of RSV-B. Although RSV-A and RSV-B co-circulated during the 2005-2006 and 2008-2009 seasons, RSV-A was predominant in the 2006-2008 seasons, whereas RSV-B was predominant in the 2009-2010 season. Upper respiratory tract infections were diagnosed in most RSV-infected patients (n = 80, 84.2%), and three patients suffered from pulmonary infection. This is the first study to provide data on the prevalence and clinical manifestations of RSV subgroups among Chinese adults with fever and acute illness, over five successive epidemic seasons. J. Med. Virol. © 2012 Wiley Periodicals, Inc.
Journal of Medical Virology 11/2012; · 2.82 Impact Factor
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ABSTRACT: The interaction between genetic factors and environmental factors has critical roles in determining the phenotype of an organism. In recent years, a number of studies have reported that the dysfunctions on microRNA (miRNAs), environmental factors and their interactions have strong effects on phenotypes and even may result in abnormal phenotypes and diseases, whereas there has been no a database linking miRNAs, environmental factors and phenotypes. Such a resource platform is believed to be of great value in the understanding of miRNAs, environmental factors, especially drugs and diseases. In this study, we constructed the miREnvironment database, which contains a comprehensive collection and curation of experimentally supported interactions among miRNAs, environmental factors and phenotypes. The names of miRNAs, phenotypes, environmental factors, conditions of environmental factors, samples, species, evidence and references were further annotated. miREnvironment represents a biomedical resource for researches on miRNAs, environmental factors and diseases. AVAILABILITY: http://cmbi.bjmu.edu.cn/miren. CONTACT: cuiqinghua@hsc.pku.edu.cn.
Bioinformatics 12/2011; 27(23):3329-30. · 5.47 Impact Factor
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Lili Ren,
Richard Gonzalez,
Zhengde Xie,
Zhaohui Xiong,
Chunyan Liu,
Zichun Xiang,
Yan Xiao,
Yongjun Li,
Hongli Zhou,
Jianguo Li, Qingqing Yang,
Jing Zhang,
Lan Chen,
Wei Wang,
Guy Vernet,
Gláucia Paranhos-Baccalà,
Kunling Shen,
Jianwei Wang
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ABSTRACT: Human parainfluenza viruses (HPIVs) are a leading cause of acute respiratory tract infections (ARTIs). Although HPIV-4 has been associated with mild ARTIs for years, recent investigations have also associated HPIV-4 infection with severe respiratory syndromes and with outbreaks of ARTIs in children.
To characterize the role of HPIV-4 and its clinical features in children with acute lower respiratory tract infections (ALRTIs) in Beijing, China.
Nasopharyngeal aspirates were collected from 2009 hospitalized children with ALRTIs between March 2007 and April 2010. RT-PCR and PCR analyses were used to identify HPIV types and other known respiratory viruses.
HPIVs were detected in 246 (12.2%) patients, of whom 25 (10.2%) were positive for HPIV-4, 11 (4.5%) for HPIV-2, 51 (20.7%) for HPIV-1, 151 (61.4%) for HPIV-3, and 8 (3.3%) were co-detected with different types of HPIVs. Like HPIV-3, HPIV-4 was detected in spring, summer, and late fall over the study period. Seasonal incidence varied for HPIV-1 and -2. The median patient age was 20 months for HPIV-4 infections and 7-11 months for HPIV-1, -2, and -3 infections, but the clinical manifestations did not differ significantly between HPIV-1, -2, -3, and -4 infections. Moreover, co-detection of HPIV-4 (44%) with other respiratory viruses was lower than that of HPIV-1 (62.7%), HPIV-2 (63.6%), and HPIV-3 (72.7%).
HPIV-4 plays an important role in Chinese paediatric ALRTIs. The epidemiological and clinical characteristics reported here improve our understanding of the pathogenesis associated with HPIV-4.
Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology 07/2011; 51(3):209-12. · 3.12 Impact Factor
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Lili Ren,
Richard Gonzalez,
Jin Xu,
Yan Xiao,
Yongjun Li,
Hongli Zhou,
Jianguo Li, Qingqing Yang,
Jing Zhang,
Lan Chen,
Wei Wang,
Guy Vernet,
Gláucia Paranhos-Baccalà,
Zhong Wang,
Jianwei Wang
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ABSTRACT: Human coronaviruses (HCoVs) are a common etiological agent of acute respiratory tract infections. HCoV infections, especially those caused by the two HCoVs identified most recently, NL63 and HKU-1, have not been characterized fully. To evaluate the prevalence and clinical presentations of HKU1 and NL63 in adults with acute respiratory tract infections, an investigation of HCoV infections in Beijing, China from 2005 to 2009 was performed by using reverse transcriptase PCR assays and sequencing analysis. Among 8,396 respiratory specimens studied, 87 (1%) clinical samples were positive for HCoVs, of which 50 samples (0.6% of the total) were positive for HCoV-OC43, 15 (0.2%) for HCoV-229E, 14 (0.2%) for HCoV-HKU1, and 8 (0.1%) for HCoV-NL63. The prevalence of HCoV infection in adults exhibited distinct seasonal fluctuations during the study period. In addition, patients positive for HCoV-229E infections were more likely to be co-infected with other respiratory viruses. Enterovirus, rhinovirus, and parainfluenza virus type 3 were the most common viruses found in patients with HCoV infections. The demographic and clinical data present in this study of HCoV infections in adults with acute respiratory tract infections should improve our understanding of the pathogenesis of HCoVs.
Journal of Medical Virology 02/2011; 83(2):291-7. · 2.82 Impact Factor
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Lili Ren,
Richard Gonzalez,
Zhengde Xie,
Yan Xiao,
Yongjun Li,
Chunyan Liu,
Lan Chen, Qingqing Yang,
Guy Vernet,
Glaucia Paranhos-Baccalà,
Qi Jin,
Kunling Shen,
Jianwei Wang
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ABSTRACT: To clarify the potential for respiratory transmission of Saffold cardiovirus (SAFV) and characterize the pathogen, we analyzed respiratory specimens from 1,558 pediatric patients in Beijing. We detected SAFV in 7 (0.5%) patients and identified lineages 1-3. However, because 3 patients had co-infections, we could not definitively say SAFV caused disease.
Emerging Infectious Diseases 07/2010; 16(7):1158-61. · 6.79 Impact Factor
