Zhongqiao Yun

Publications (3)8.55 Total impact

• Article: Molecular epidemiological study of hepatitis B virus in blood donors from five Chinese blood centers.

  Yu Liu, Jingxing Wang, Yi Huang, Tonghan Yang, Xiaoming Guo, Julin Li, Guoxin Wen, Zhongqiao Yun, Peibin Zeng, Miao He, Min Xu, Gui Liu, Ling Ke, David Wright, Jing Liu, Kenrad Nelson, Hua Shan
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  ABSTRACT: Although the genetic variability of hepatitis B virus (HBV) in HBV-infected patients has been extensively studied, reports on genotypes, subtypes and mutations in the S region of HBV strains from Chinese blood donors are limited. In this study, 245 blood samples from HBsAg-positive blood donors were collected from five geographically diverse blood centers in China. The S region of HBV was amplified, and the HBV genotype and subtype were determined. The amino acid sequences of the S region were aligned, and mutations related to the failure of immunization and HBsAg detection were determined. Of the 245 samples, 228 (93 %) were genotyped successfully. We found that genotypes B, C, D and A accounted for 58.8 %, 21.9 %, 6.6 % and 3.95 % of the isolates, respectively. The distribution of HBV antigen subtypes was as follows: adw (67.6 %), adr (23.3 %) and ayw (8.7 %). Mutations were present in 39 (17.1 %) of 228 samples in the major hydrophilic region (MHR) of the S region. This study demonstrated that HBV genotype/subtype B/adw was the most frequent strain circulating in HBV-infected Chinese blood donors, followed by C/adr. The occurrence of MHR mutants in HBV-infected blood donors and the potential failure to detect some of them in collected units poses a threat to transfusion safety.
  Archives of Virology 06/2012; 157(9):1699-707. · 2.11 Impact Factor
• Article: The human immunodeficiency virus-1 genotype diversity and drug resistance mutations profile of volunteer blood donors from Chinese blood centers.

  Peibin Zeng, Jingxing Wang, Yi Huang, Xiaoming Guo, Julin Li, Guoxin Wen, Tonghan Yang, Zhongqiao Yun, Miao He, Yu Liu, Yuzhe Yuan, Jane Schulmann, Simone Glynn, Paul Ness, J Brooks Jackson, Hua Shan
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  ABSTRACT: The global human immunodeficiency virus (HIV)-1 epidemic is becoming increasingly diverse and complex. Molecular epidemiologic characteristics were studied for HIV-1-infected blood donors from five Chinese regions to determine genotype diversity and drug resistance mutations (DRMs) profile. HIV-1 confirmed-reactive serum samples were collected from 172 blood donors from five blood centers during 2007 to 2010. HIV-1 Pol including whole protease and partial reverse transcriptase genes was amplified, sequenced, and analyzed for the subtype determination and drug resistance profile description. A total of 113 amplified sequences including 82 from Kunming blood center and 31 from four other blood centers had the following genotype characteristics: G (0.9%), B (2.7%), circulating recombinant form (CRF) 01_AE (32.7%), CRF07_BC (22.1%), and CRF08_BC (41.6%). Female donors represent 45.1% of all cases and 63.9% cases with DRMs. The prevalence of samples with potential low or higher resistance among Chinese blood donors is 4.4%. HIV-1 infection in Chinese blood donors is genetically diverse and the subtype distribution reflects that from the high-risk populations. Our results support continuous molecular epidemiologic surveillance for HIV-1 in blood donors as a part of a comprehensive HIV control program.
  Transfusion 11/2011; 52(5):1041-9. · 3.22 Impact Factor
• Article: Who donates blood at five ethnically and geographically diverse blood centers in China in 2008.

  Jingxing Wang, Nan Guo, Xiaoming Guo, Julin Li, Guo-Xing Wen, Tonghan Yang, Zhongqiao Yun, Yi Huang, George B Schreiber, Katherine Kavounis, Paul Ness, Hua Shan
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  ABSTRACT: In China recruitment and retention of sufficient numbers of safe blood donors continues to be a challenge. Understanding who donates blood, particularly those who donate larger (>200 mL) whole blood (WB) units, will help blood centers to target more effective recruitment and retention strategies. Demographic characteristics of 226,489 allogeneic WB donors from January to December 2008 at five geographically and ethnically diverse, urban blood centers were analyzed. The typical Chinese WB donor can be characterized as first-time volunteer (67.9%), male (56.9%), less than 45 years old (93.8%), and Han ethnicity (86.1%). Most donors had some college or below educational level (77.5%), donated at a mobile collection site (97.6%), and donated 300- or 400-mL units (76.0%). Differences in WB volume donations and donor demographics exist among the five centers. In China compared to the United States, donations are made by younger donors and donors give infrequently and make smaller WB donations. To help ensure supply adequacy, continued efforts are needed to have donors give larger volumes of WB in China.
  Transfusion 12/2010; 50(12):2686-94. · 3.22 Impact Factor