Publications (9)21.63 Total impact
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Article: Identification of a new HLA-DRB1*11 variant, HLA-DRB1*11:130, by sequence-based typing in a Brazilian individual.
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ABSTRACT: HLA-DRB1*11:130, detected in a Euro-Brazilian female, presents a point mutation at codon 59.3 (GAG→GAC).Tissue Antigens 03/2013; · 2.59 Impact Factor -
Article: HLA class II polymorphisms and recurrent spontaneous abortion in a Southern Brazilian cohort.
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ABSTRACT: A high proportion of human recurrent spontaneous abortions (RSA) remain unexplained. The possible association between RSA and different genetic polymorphisms within the human leucocyte antigen system (HLA system, the human major histocompatibility complex) has been investigated with conflicting results since many decades. Here, we describe a case-control study with 136 Southern Brazilian women of predominantly European ancestry (75 control and 61 cases with unexplained RSA). We investigated the relationship between unexplained RSA and alleles and genotypes from two classical loci of the HLA: HLA-DRB1 and HLA-DQB1, as well as three loci related to cytokine production and their serum levels: TNFA (-308G>A), IL10 (-1082G>A, -819T>C, -592A>C) and IFNG (+874A>T). Genotyping was performed by an allele-specific PCR method. While all results concerning cytokine-related genes turned out to be negative, we found the genotype HLA-DQB1*02:02, 03:01 to be significantly decreased and the allele HLA-DRB1*11:04 to be significantly increased among patients.International Journal of Immunogenetics 09/2012; · 1.29 Impact Factor -
Article: HLA-DRB1*08:48, a novel allele identified in a Brazilian donor.
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ABSTRACT: The new allele presents a point mutation at codon 67.1 (ATC→CTC) resulting in a conservative change from isoleucine to leucine.Tissue Antigens 03/2012; 79(5):391-2. · 2.59 Impact Factor -
Article: A novel allele, HLA-A*66:14, identified in a Brazilian volunteer bone marrow donor.
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ABSTRACT: The new allele might have arisen from HLA-A*66:01 through a point mutation at codon 182.1 (ACG→GCG) resulting in a non-conservative change from threonine to alanine.Tissue Antigens 12/2011; 79(3):207-8. · 2.59 Impact Factor -
Article: Two novel alleles, HLA-B*40:125 and B*40:129, in the Brazilian population.
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ABSTRACT: The new alleles, HLA-B*40:125 and HLA-B*40:129, present a mismatch at codon 103.1 (G → C) and three mismatches at codons 9.1 (C → T), 11.1 (T → G) and 12.1 (G → A).Tissue Antigens 11/2011; 79(2):137-8. · 2.59 Impact Factor -
Article: Recombinant DNA immunotherapy ameliorate established airway allergy in a IL-10 dependent pathway.
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ABSTRACT: Previous studies have established that mycobacterial infections ameliorate allergic inflammation. However, a non-infectious approach that controls allergic responses might represent a safer and more promising strategy. The 60-65 kDa heat shock protein (Hsp) family is endowed with anti-inflammatory properties, but it is still unclear whether and how single mycobacterial Hsp control allergic disorders. Therefore, in this study we determined whether the administration of Mycobacterial leprae Hsp65 expressed by recombinant a DNA plasmid could attenuate a previously established allergic response. We used an experimental model of airway allergic inflammation to test the effects of immunotherapy with DNA encoding Hsp65. Allergic mice, previously sensitized and challenged with ovalbumin, were treated with tree intramuscular doses of recombinant DNA encoding Hsp65. After treatment, mice received a second allergen challenge and the allergic response was measured. We found that immunotherapy attenuated eosinophilia, pulmonary inflammation, Th2 cytokine and mucus production. Moreover, we showed that the inhibition of allergic response is dependent on IL-10 production. Both Hsp65 and allergen-specific IL-10-producing cells contributed to this effect. Cells transferred from DNA-immunized mice to allergic mice migrated to allergic sites and down-modulated the Th2 response. Our findings clearly show that immunotherapy with DNA encoding Hsp65 can attenuate an established Th2 allergic inflammation through an IL-10-dependent mechanism; moreover, the migration of allergen- and Hsp65-specific cells to the allergic sites exerts a fundamental role. This work represents a novel contribution to the understanding of immune regulation by Hsp65 in allergic diseases.Clinical & Experimental Allergy 09/2011; 42(1):131-43. · 5.03 Impact Factor -
Article: A new allele, HLA-B*4212, identified in a Brazilian volunteer bone marrow donors by sequence-based typing.
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ABSTRACT: Here we report the discovery of a novel HLA-B allele, named B*4212 in a Brazilian volunteer bone marrow donor. The new sequence has nucleotide variation at position 496 (T→G) as compared with B*4201. This variation results in a conservative amino acid substitution from valine to glycine at codon 165 of exon 3.International Journal of Immunogenetics 12/2010; 37(6):517-8. · 1.29 Impact Factor -
Article: HLA-B*18:35, a new HLA-B*18 allele identified by sequence-based typing in a Brazilian volunteer bone marrow donor.
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ABSTRACT: A novel human leukocyte antigen-B allele named B*18:35 was identified in a Brazilian volunteer bone marrow donor.Tissue Antigens 10/2010; 76(4):336-7. · 2.59 Impact Factor -
Article: Leukotrienes are not essential for the efficacy of a heterologous vaccine against Mycobacterium tuberculosis infection.
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ABSTRACT: Leukotrienes are reported to be potent proinflammatory mediators that play a role in the development of several inflammatory diseases such as asthma, rheumatoid arthritis and periodontal disease. Leukotrienes have also been associated with protection against infectious diseases. However, the role of leukotrienes in Mycobacterium tuberculosis infection is not understood. To answer this question, we studied the role of leukotrienes in the protective immune response conferred by prime-boost heterologous immunization against tuberculosis. We immunized BALB/c mice (4-11/group) with subcutaneous BCG vaccine (1 x 10(5) M. bovis BCG) (prime) followed by intramuscular DNA-HSP65 vaccine (100 microg) (boost). During the 30 days following the challenge, the animals were treated by gavage daily with MK-886 (5 mg x kg(-1) x day(-1)) to inhibit leukotriene synthesis. We showed that MK-886-treated mice were more susceptible to M. tuberculosis infection by counting the number of M. tuberculosis colony-forming units in lungs. The histopathological analysis showed an impaired influx of leukocytes to the lungs of MK-886-treated mice after infection, confirming the involvement of leukotrienes in the protective immune response against experimental tuberculosis. However, prime-boost-immunized mice treated with MK-886 remained protected after challenge with M. tuberculosis, suggesting that leukotrienes are not required for the protective effect elicited by immunization. Protection against M. tuberculosis challenge achieved by prime-boost immunization in the absence of leukotrienes was accompanied by an increase in IL-17 production in the lungs of these animals, as measured by ELISA. Therefore, these data suggest that the production of IL-17 in MK-886-treated, immunized mice could contribute to the generation of a protective immune response after infection with M. tuberculosis.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas / Sociedade Brasileira de Biofisica ... [et al.] 07/2010; 43(7):645-50. · 1.08 Impact Factor
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Institutions
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2010–2013
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Universidade Federal do Paraná
- Departamento de Genética
Curitiba, Estado do Parana, Brazil
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