Lianke Liu

Publications (5)10.3 Total impact

• Article: Silver nanocrystals mediated combination therapy of radiation with magnetic hyperthermia on glioma cells.

  Hua Jiang, Changling Wang, Zhirui Guo, Zhaoxia Wang, Lianke Liu
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  ABSTRACT: Based on the study of apoptosis-induced and anti-proliferation behavior of silver nanoparticles (AgNPs) on cancer cells, the attractively therapeutic effect and potential application of AgNPs in anti-cancer field was gradually revealed. Here we investigated the effect of 10 nm silver nanoparticles (AgNPs) on human glioma U251 cells upon the combination treatment of ionizing radiation (IR) treatment with magnetic hyperthermia (MHT). AgNPs showed both radio and thermo sensitivity on U251 cells from the surviving fraction curve. Besides, we found both X-rays and heat could enhance the content of cells uptake of AgNPs. As the amount of intracellular AgNPs accumulated, the apoptosis rate of U251 cells enhanced. Furthermore, we established a simplified model for calculating cell survival rate and demonstrated that after RT, MHT and RT combined with MHT, AgNPs could significantly inhibited cancer cell proliferation. Our results revealed that AgNPs could have a potential application in enhancing effect of RT with MHT combination therapy induced killing of cancer cells.
  Journal of Nanoscience and Nanotechnology 11/2012; 12(11):8276-81. · 1.56 Impact Factor
• Article: Thermal analysis in the rat glioma model during directly multipoint injection hyperthermia incorporating magnetic nanoparticles.

  Lianke Liu, Fang Ni, Jianchao Zhang, Chunyu Wang, Xiang Lu, Zhirui Guo, Shaowei Yao, Yongqian Shu, Ruizhi Xu
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  ABSTRACT: Hyperthermia incorporating magnetic nanoparticles (MNPs) is a hopeful therapy to cancers and steps into clinical tests at present. However, the clinical plan of MNPs deposition in tumors, especially applied for directly multipoint injection hyperthermia (DMIH), and the information of temperature rise in tumors by DMIH is lack of studied. In this paper, we mainly discussed thermal distributions induced by MNPs in the rat brain tumors during DMIH. Due to limited experimental measurement for detecting thermal dose of tumors, and in order to acquire optimized results of temperature distributions clinically needed, we designed the thermal model in which three types of MNPs injection for hyperthermia treatments were simulated. The simulated results showed that MNPs injection plan played an important role in determining thermal distribution, as well as the overall dose of MNPs injected. We found that as injected points enhanced, the difference of temperature in the whole tumor volume decreased. Moreover, from temperature detecting data by Fiber Optic Temperature Sensors (FOTSs) in glioma bearing rats during MNPs hyperthermia, we found the temperature errors by FOTSs reduced as the number of points injected enhanced. Finally, the results showed that the simulations are preferable and the optimized plans of the numbers and spatial positions of MNPs points injected are essential during direct injection hyperthermia.
  Journal of Nanoscience and Nanotechnology 12/2011; 11(12):10333-8. · 1.56 Impact Factor
• Article: Silver nanocrystals sensitize magnetic-nanoparticle-mediated thermo-induced killing of cancer cells.

  Lianke Liu, Fang Ni, Jianchao Zhang, Xiaoli Jiang, Xiang Lu, Zhirui Guo, Ruizhi Xu
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  ABSTRACT: Magnetic nanoparticles (MNPs) can heat up tumor tissues and induce killing of cancer cells under external AC magnetic field. However, magnetic nanoparticles hyperthermia (MNPH) requires high concentration of MNPs that are injected into the tumor in order to obtain clinically needed thermal dose because of the complicated heat transfer in vivo and the limited heat quality of MNPs. To cut down the dose of MNPs and enhance the effect of this Nanotherapy, we prepared silver nanoparticles (AgNPs) with different sizes and investigated the effects of these AgNPs on cancer cells in MNPH treatment. It was found that AgNPs could enhance thermo-sensitivity of glioma cells and this effect was size dependent. AgNPs could induce cell cycles arrested in G(2)/M phase and enhanced the apoptosis rate of cancer cells after hyperthermia. In glioma bearing rats model, MNPH combined with AgNPs could enhance Bax expression in cancer cells. Our results suggested that AgNPs could be a potential thermo-sensitizer and could be further developed for the design of Ag nanostructure-based thermal seeds for MNPH therapy.
  Acta Biochimica et Biophysica Sinica 03/2011; 43(4):316-23. · 1.38 Impact Factor
• Source

  Available from: PubMed Central

  Article: Facile purification of colloidal NIR-responsive gold nanorods using ions assisted self-assembly.

  Lianke Liu, Zhirui Guo, Lina Xu, Ruizhi Xu, Xiang Lu
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  ABSTRACT: Anisotropic metal nanoparticles have been paid much attention because the broken symmetry of these nanoparticles often leads to novel properties. Anisotropic gold nanoparticles obtained by wet chemical methods inevitably accompany spherical ones due to the intrinsically high symmetry of face-centred cubic metal. Therefore, it is essential for the purification of anisotropic gold nanoparticles. This work presents a facile, low cost while effective solution to the challenging issue of high-purity separation of seed-mediated grown NIR-responsive gold nanorods from co-produced spherical and cubic nanoparticles in solution. The key point of our strategy lies in different shape-dependent solution stability between anisotropic nanoparticles and symmetric ones and selective self-assembly and subsequent precipitation can be induced by introducing ions to the as-made nanorod solution. As a result, gold nanorods of excellent purity (97% in number density) have been obtained within a short time, which has been confirmed by SEM observation and UV-vis-NIR spectroscopy respectively. Based on the experimental facts, a possible shape separation mechanism was also proposed.
  Nanoscale Research Letters 01/2011; 6(1):143. · 2.73 Impact Factor
• Article: Achieving high-purity colloidal gold nanoprisms and their application as biosensing platforms.

  Zhirui Guo, Xu Fan, Lianke Liu, Zhiping Bian, Chunrong Gu, Yu Zhang, Ning Gu, Di Yang, Jinan Zhang
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  ABSTRACT: Gold nanoprisms with average edge size of approximately 140 nm and thickness of approximately 8 nm were achieved in high-purity ( approximately 97%) by exploiting the electrostatic aggregation and shape effects through a modified seed-mediated approach. The proposed strategy lies in the dramatically different stability and aggregation potential between the produced gold nanoprisms and spherical gold nanoparticles, which can be modulated by varying the anion concentration in the reaction solution. Hence, the gold nanoprisms spontaneously aggregated into precipitate whereas most of the spherical ones were still kept in the solution. Moreover, this strategy is also flexible enough that ultra-small gold nanoprisms with average width less than 50 nm can be collected in good-purity. The structure and optical properties of these nanoprisms have been studied by TEM, SAED, XRD and UV-vis-NIR spectroscopy, respectively. These high-purity colloidal gold nanoprisms exhibit remarkably enhanced surface plasmon resonance (SPR) as well as strong near-infrared absorption. Furthermore, we have also investigated their potential for biosensing based on the sensitive changes of SPR band induced by the antibody-antigen recognition events. The experimental results clearly suggest that gold nanoprisms can be a promising nanostructured system for plasmonic sensor applications.
  Journal of Colloid and Interface Science 08/2010; 348(1):29-36. · 3.07 Impact Factor