Anizah Rahumatullah

Publications (3)7.31 Total impact

• Article: Triplex PCR using new primers for the detection of Toxoplasma gondii.

  Anizah Rahumatullah, Boon Yin Khoo, Rahmah Noordin
  [show abstract] [hide abstract]
  ABSTRACT: Molecular methods are used increasingly for the detection of Toxoplasma gondii infection. This study developed a rapid, sensitive, and specific conventional triplex PCR for the detection of the B1 gene and ITS1 region of T. gondii using newly designed primers and an internal control based on the Vibrio cholerae HemM gene. The annealing temperature and concentrations of the primers, MgCl(2), and dNTPs were optimized. Two sets of primers (set 1 and 2) were tested, which contained different segments of the T. gondii B1 gene, 529 repeat region and ITS1 region. A series of sensitivity tests were performed using parasite DNA, whole parasites, and spiked human body fluids. Specificity tests were performed using DNA from common protozoa and bacteria. The newly developed assay based on set 2 primers was found to be specific and sensitive. The test was capable of detecting as little as 10 pg T. gondii DNA, 10(4) tachyzoites in spiked body fluids, and T. gondii DNA in the organ tissues of experimentally infected mice. The assay developed in this study will be useful for the laboratory detection of T. gondii infection.
  Experimental Parasitology 04/2012; 131(2):231-8. · 2.12 Impact Factor
• Article: A pentaplex real-time polymerase chain reaction assay for detection of four species of soil-transmitted helminths.

  Madihah Basuni, Jamail Muhi, Nurulhasanah Othman, Jaco J Verweij, Maimunah Ahmad, Noorizan Miswan, Anizah Rahumatullah, Farhanah Abdul Aziz, Nurul Shazalina Zainudin, Rahmah Noordin
  [show abstract] [hide abstract]
  ABSTRACT: Soil-transmitted helminth infections remain a major public health burden in low- and middle-income countries. The traditional diagnosis by microscopic examination of fecal samples is insensitive and time-consuming. In this study, a pentaplex real-time polymerase chain reaction (PCR) was evaluated for the simultaneous detection of Ancylostoma, Necator americanus, Ascaris lumbricoides, and Strongyloides stercoralis. The results were compared with those obtained by conventional parasitological diagnostic methods. Real-time PCR was positive in 48 of 77 samples (62.3%) and microscopic examination was positive in six samples (7.8%) only (P < 0.05). In conclusion, the real-time PCR assay described in this study provides a specific and sensitive diagnostic tool for the detection of these four helminth species in epidemiological studies and monitoring of treatment programs.
  The American journal of tropical medicine and hygiene 02/2011; 84(2):338-43. · 2.59 Impact Factor
• Source

  Available from: PubMed Central

  Article: Potential effects of chrysin on MDA-MB-231 cells.

  Teh Ban Hong, Anizah Rahumatullah, Thaneswary Yogarajah, Maimunah Ahmad, Khoo Boon Yin
  [show abstract] [hide abstract]
  ABSTRACT: This study aims to elucidate the effects of chrysin on human ER-negative breast cancer cell line, MDA-MB-231. The study demonstrated that treatment of MDA-MB-231 cells with 20 microM chysin for 48 h significantly inhibited the growth of MDA-MB-231 cells and induced cytoplasmic lipid accumulation in the cells, but that the observed of cell death was not caused by apoptosis. The expression of PPARalpha mRNA in chrysin-treated MDA-MB-231 cells was significantly increased, which was likely associated to the proliferation of the cells post chrysin treatment.
  International Journal of Molecular Sciences 01/2010; 11(3):1057-69. · 2.60 Impact Factor