R.C. Frohberg

Publications (10)6.59 Total impact

• Chapter: Breeding Hard Red Spring Wheat for Fusarium Head Blight Resistance

  M. Mergoum, R. C. Frohberg, R. W. Stack
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  ABSTRACT: Fusarium head blight (FHB) or scab (caused by Fusarium graminearum Schwabe [telomorph Gibberella zeae (Schwein.) Petch]) is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide, causing significant reductions in grain yield and quality. In the USA, since the 1993 FHB epidemic, tremendous research efforts have been undertaken to address this major problem that causes substantial economic losses for the wheat growers, industry, and export market. The deployment of resistant varieties is the only effective, economical and environmentally safe way to control FHB in wheat. More than a decade of classical breeding efforts to develop scab resistant hard red spring wheat (HRSW) cultivars at North Dakota State University (NDSU) resulted in releasing several HRSW cultivars with varying levels of FHB resistance. Since 2000, three major HRSW cultivars with FHB resistance were released and grown on large scale in the Northern-Central plains of the USA. These are ‘Alsen’ (2000), ‘Steele-ND’ (2004), and ‘Glenn’ (2005). Alsen has been grown, in average, on more than one million hectares in the last 3 years. However, Alsen and most FHB resistant wheat cultivars released by other wheat programs in the USA, trace back to the Chinese line ‘Sumai-3’ (PI481542) or its derivatives. The HRSW breeding program at NDSU has invested substantial breeding efforts to identify, introgress novel resistance genes from other sources to enhance genetic diversity, and facilitate pyramiding these resistance genes. The ultimate objective is developing HRSW cultivars with effective and durable FHB resistance. Our efforts have yielded in releasing the HRSW cultivars ‘‘Steele-ND’’ in 2004 and ‘‘Glenn’’ in 2005. Steele-ND traces its resistance to the wheat relative species Triticum dicoccoides and Glenn combines both Alsen and Steele-ND resistances. This paper addresses the breeding efforts at NDSU to release FHB resistant HRSW cultivars and elite germplasm as parental material for many breeding programs worldwide; and the future challenges to keep our research efforts ahead of the FHB disease
  05/2007: pages 161-167;
• Source

  Available from: Shahryar F Kianian

  Article: Detection of QTL linked to Fusarium head blight resistance in Sumai 3-derived North Dakota bread wheat lines.

  I A del Blanco, R C Frohberg, R W Stack, W A Berzonsky, S F Kianian
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  ABSTRACT: During the past decade Fusarium head blight (FHB) caused by Fusarium graminearum Schwabe has resulted in severe grain yield and quality losses of wheat (Triticum aestivum L.) in the Northern Great Plains of the U.S. Given the complexity of breeding for FHB resistance, molecular markers associated with this trait will be valuable in accelerating efforts to breed resistant cultivars. The objective of this study was to identify molecular markers linked to quantitative trait loci (QTL) for FHB resistance in wheat using a set of lines obtained by several cycles of crossing to North Dakota adapted genotypes, which derived their resistance from cv. Sumai 3. Microsatellite markers spanning the wheat genome were used to screen parents and derived lines. Polymorphisms for parental alleles were compared to disease scores for Type II resistance. The probability of linkage between markers and introgressed resistance genes was calculated using a binomial probability formula based on the assumption that a molecular marker at a specific distance from the introgressed gene, in a near-isogenic line (NIL), will carry the donor-parent allele as a function of the distance between marker and gene and the number of backcrosses/selfs performed in deriving the NIL. Microsatellite loci Xgwm533 and Xgwm274 were significantly associated with QTL for FHB resistance.
  Theoretical and Applied Genetics 05/2003; 106(6):1027-31. · 3.30 Impact Factor
• Source

  Available from: ddr.nal.usda.gov

  Article: DNA markers for Fusarium head blight resistance QTLs in two wheat populations

  J. A. Anderson, R. W. Stack, S. Liu, B. L. Waldron, A. D. Fjeld, C. Coyne, B. Moreno-Sevilla, J. Mitchell Fetch, Q. J. Song, P. B. Cregan, R. C. Frohberg
  [show abstract] [hide abstract]
  ABSTRACT: Genetic resistance to Fusarium head blight (FHB), caused by Fusarium graminearum, is necessary to reduce the wheat grain yield and quality losses caused by this disease. Development of resistant cultivars has been slowed by poorly adapted and incomplete resistance sources and confounding environmental effects that make screening of germplasm difficult. DNA markers for FHB resistance QTLs have been identified and may be used to speed the introgression of resistance genes into adapted germplasm. This study was conducted to identify and map additional DNA markers linked to genes controlling FHB resistance in two spring wheat recombinant inbred populations, both segregating for genes from the widely used resistance source ’Sumai 3’. The first population was from the cross of Sumai 3/Stoa in which we previously identified five resistance QTLs. The second population was from the cross of ND2603 (Sumai 3/Wheaton) (resistant)/ Butte 86 (moderately susceptible). Both populations were evaluated for reaction to inoculation with F. graminearum in two greenhouse experiments. A combination of 521 RFLP, AFLP, and SSR markers were mapped in the Sumai 3/Stoa population and all DNA markers associated with resistance were screened on the ND2603/Butte 86 population. Two new QTL on chromosomes 3AL and 6AS wer found in the ND2603/Butte 86 population, and AFLP and SSR markers were identified that explained a greater portion of the phenotypic variation compared to the previous RFLP markers. Both of the Sumai 3-derived QTL regions (on chromosomes 3BS, and 6BS) from the Sumai 3/Stoa population were associated with FHB resistance in the ND2603/Butte 86 population. Markers in the 3BS QTL region (Qfhs.ndsu-3BS) alone explain 41.6 and 24.8% of the resistance to FHB in the Sumai 3/Stoa and ND2603/Butte 86 populations, respectively. This region contains a major QTL for resistance to FHB and should be useful in marker-assisted selection.
  Theoretical and Applied Genetics 05/2001; 102(8):1164-1168. · 3.30 Impact Factor
• Article: Registration of 'Glenn' wheat.

  M. Mergoum, R.C. Frohberg, R.W. Stack, T. Olson, T.L. Friesen, J.B. Rasmussen
• Article: Registration of spring wheat germplasm ND 735 combining tan spot, leaf, and stem rusts.

  M. Mergoum, R.C. Frohberg, S Ali, P K Singh, J.B. Rasmussen, J.D. Miller
• Article: Registration of 'Howard' Wheat.

  M. Mergoum, R.C. Frohberg, R.W. Stack, J.B. Rasmussen, T.L. Friesen
• Article: Registration of 'Alsen' Wheat.

  R.C. Frohberg, R.W. Stack, T. Olson, J.D. Miller, M. Mergoum
• Article: Registration of spring wheat germplasm ND 652 resistant to common root rot, leaf, and stem rusts.

  M. Mergoum, R.C. Frohberg, R.W. Stack, N. Riveland, T. Olson, J.D. Miller
• Article: Registration of spring wheat germplasm ND 744 resistant to Fusarium head blight, leaf, and stem rusts.

  M. Mergoum, R.C. Frohberg, J.D. Miller, J.B. Rasmussen, R.W. Stack
• Article: DNA markers for Fusarium head blight resistance QTLs in two wheat popoulations.

  J A Anderson, R.W. Stack, S Liu, B.L. Waldron, A.D. Fjeld, C. Coyne, B. Moreno-Sevilla, J.M. Fetch, Q J Song, P.B. Cregan, R.C. Frohberg
  [show abstract] [hide abstract]
  ABSTRACT: Genetic resistance to Fusarium head blight (FHB), caused by Fusarium graminearum, is necessary to reduce the wheat grain yield and quality losses caused by this disease. Development of resistant cultivars has been slowed by poorly adapted and incomplete resistance sources and confounding environmental effects that make screening of germplasm difficult. DNA markers for FHB resistance QTLs have been identified and may be used to speed the introgression of resistance genes into adapted germplasm. This study was conducted to identify and map additional DNA markers linked to genes controlling FHB resistance in two spring wheat recombinant inbred populations, both segregating for genes from the widely used resistance source 'Sumai 3'. The first population was from the cross of Sumai 3/Stoa in which we previously identified five resistance QTLs. The second population was from the cross of ND2603 (Sumai 3/Wheaton) (resistant)/ Butte 86 (moderately susceptible). Both populations were evaluated for reaction to inoculation with F. graminearum in two greenhouse experiments. A combination of 521 RFLP, AFLP, and SSR markers were mapped in the Sumai 3/Stoa population and all DNA markers associated with resistance were screened on the ND2603/Butte 86 population. Two new QTL on chromosomes 3AL and 6AS were found in the ND2603/Butte 86 population, and AFLP and SSR markers were identified that explained a greater portion of the phenotypic variation compared to the previous RFLP markers. Both of the Sumai 3-derived QTL regions (on chromosomes 3BS, and 6BS) from the Sumai 3/Stoa population were associated with FHB resistance in the ND2603/Butte 86 population. Markers in the 3BS QTL region (Qfhs.ndsu-3BS) alone explain 41.6 and 24.8% of the resistance to FHB in the Sumai 3/Stoa and ND2603/Butte 86 populations, respectively. This region contains a major QTL for resistance to FHB and should be useful in marker-assisted selection.