Xiaoli Sun

Capital Medical University, Peping, Beijing, China

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Publications (12)26.41 Total impact

  • Xiaoli Sun · Cheng Liu · Rengui Wang · Dailun Hou · Jiuhong Chen
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    ABSTRACT: To evaluate the clinical value of dual source computed tomography (DSCT) angiography in the diagnosis and treatment for popliteal artery entrapment syndrome (PAES). 8 patients with PAES were retrospectively reviewed. 64-slice dual source CT angiography was performed based on the following protocol: 100 mL of Iopamidol (370 mgI/mL) was injected at a rate of 3.5 mL/s and arterial phase images were obtained by using bolus tracking. Axial DSCT images and reconstructed images including multi-planar reconstruction (MPR), maximum intensity projection (MIP), volume rendering (VR) were collected and analysed. All patients underwent Doppler colour ultrasound examinations and surgeries. The popliteal artery and the neighbouring muscular structures were clearly shown on the axial images revealing the cause of the arterial entrapment. Furthermore, the site and length of the segmental occlusion and collateral developments were well demonstrated on reconstructed images. Characterisation and classification based on DSCT angiography were confirmed by surgeries. PAES was accurately diagnosed by DSCT angiography in all enrolled patients. In contrary, only 5 PAES cases were accurately diagnosed by ultrasound examination. DSCT angiography is a noninvasive and valuable tool in the diagnosis of PAES and plays an important role in the determination of treatment plans.
    Journal of Medical Imaging and Radiation Oncology 04/2013; 57(2):156-60. DOI:10.1111/j.1754-9485.2012.02465.x · 0.95 Impact Factor
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    ABSTRACT: PURPOSE To evaluate the accuracy of 256iCT angiography in patients with peripheral artery in-stent restenosis, with conventional digital subtraction angiography (DSA) as the reference standard. METHOD AND MATERIALS This study was approved by local ethics committee, and written informed consent was obtained from all patients. Between March 2010 and December 2011, a total of 36 patients (31 men, 5 women; mean age, 68.1±8.5 years) with symptomatic peripheral arterial occlusive disease after peripheral artery stent (72 stented lesions) underwent both 256iCT angiography and conventional DSA. Each stent was classified as evaluable or unevaluable, and every stent was divided into three segments (proximal stent, stent body, and distal stent), resulting in 216 segments. For evaluation, stenosis was graded as follows: 1, none or slight stenosis (<25%); 2, mild stenosis (25-49%); 3, moderate stenosis (50-74%); 4, severe stemosis or total occlusion (≥75%). Two readers evaluated all CT angiograms with regard to narrowing of in-stent restenosis by consensus. Results were compared with findings of the DSA. RESULTS Of 72 stents, 58(80.6%) were determined to be assessable. The metal artifact of the gold marker and motion artifact increased uninterpretability of the images of stents. In evaluable stents, 28 of 32 in-stent restenosis were correctly detected by 256iCT (87.5% sensitivity). Additionally, there was no significant difference between 256iCT angiograms and DSA. CONCLUSION 256iCT angiography has a high accuracy for the detection of significant in-stent restenosis of assessable stents in patients with peripheral artery stent implantation, and therefore can be considered as a valuable noninvasive technique for stent surveillance. CLINICAL RELEVANCE/APPLICATION 256iCT angiography can be considered as a valuable noninvasive technique for stent surveillance.
    Radiological Society of North America 2012 Scientific Assembly and Annual Meeting; 11/2012
  • Xiaoli Sun · Chunxi Liu · Donghua Liu · Peng Li · Na Zhang
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    ABSTRACT: Low cytotoxicity and high transfection efficiency are critical issues in designing current non-viral gene delivery vectors. In the present study, a novel biomimetic lipid-polycation copolymer, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-graft-poly(l-lysine)-block-poly(ethylene glycol) (DOPE-g-PLL-b-PEG) was first synthesized and the potential of this novel hybrid lipid-polycation as efficient gene vector was further evaluated. DOPE-g-PLL-b-PEG and DNA could self-assemble into lipid modified polyion complex micelles (LPCM) through electrostatic interactions. Compared with PEG-b-PLL/DNA polyion complex micelles (PIC), LPCM could protect DNA from plasma, nuclease degradation in vitro and showed lower cytotoxicity to HepG2 and HeLa cells (P<0.05). The results of transfection study in vitro indicated that LPCM exhibited higher gene expression than PIC. Especially, the corresponding LPCM displayed the highest transfection efficiency in HeLa cells (P<0.05) when DOPE grafting ratio reached up to 30%. These results suggested that LPCM could facilitate gene transfer in cultured cells and might alleviate the drawbacks of the conventional cationic vector/DNA complexes. As a novel hybrid lipid-polycation, DOPE-g-PLL-b-PEG was valuable to be evaluated for its further application as gene carrier in vivo.
    International Journal of Pharmaceutics 04/2012; 425(1-2):62-72. DOI:10.1016/j.ijpharm.2012.01.010 · 3.65 Impact Factor
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    ABSTRACT: The success of gene therapy asks for the development of multifunctional vectors that could overcome various gene delivery barriers, such as the cell membrane, endosomal membrane, and nuclear membrane. Layer-by-layer technique is an efficient method with easy operation which can be used for the assembly of multifunctional gene carriers. This work describes a pH-sensitive multifunctional gene vector that offered long circulation property but avoided the inhibition of tumor cellular uptake of gene carriers associated with the use of polyethylene glycol. Deoxyribonucleic acid (DNA) was firstly condensed with protamine into a cationic core which was used as assembly template. Then, additional layers of anionic DNA, cationic liposomes, and o-carboxymethyl-chitosan (CMCS) were alternately adsorbed onto the template via layer-by-layer technique and finally the multifunctional vector called CMCS-cationic liposome-coated DNA/protamine/DNA complexes (CLDPD) was constructed. For in vitro test, the cytotoxicity and transfection investigation was carried out on HepG2 cell line. For in vivo evaluation, CMCS-CLDPD was intratumorally injected into tumor-bearing mice and the tumor cells were isolated for fluorescence determination of transfection efficiency. CMCS-CLDPD had ellipsoidal shapes and showed "core-shell" structure which showed stabilization property in serum and effective protection of DNA from nuclease degradation. In vitro and in vivo transfection results demonstrated that CMCS-CLDPD had pH-sensitivity and the outermost layer of CMCS fell off in the tumor tissue, which could not only protect CMCS- CLDPD from serum interaction but also enhance gene transfection efficiency. These results demonstrated that multifunctional CMCS-CLDPD had pH- sensitivity, which may provide a new approach for the antitumor gene delivery.
    International Journal of Nanomedicine 02/2012; 7:925-39. DOI:10.2147/IJN.S26955 · 4.38 Impact Factor
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    ABSTRACT: Low cytotoxicity and high gene transfection efficiency are critical issues in designing current non-viral gene delivery vectors. The purpose of the present work was to synthesize the novel biodegradable poly (lactic acid)-poly(ethylene glycol)-poly(l-lysine) (PLA-PEG-PLL) copolymer, and explore its applicability and feasibility as a non-viral vector for gene transport. PLA-PEG-PLL was obtained by the ring-opening polymerization of Lys(Z)-NCA onto amine-terminated NH(2)-PEG-PLA, then acidolysis to remove benzyloxycarbonyl. The tri-block copolymer PLA-PEG-PLL combined the characters of cationic polymer PLL, PLA and PEG: the self-assembled nanoparticles (NPs) possessed a PEG loop structure to increase the stability, hydrophobic PLA segments as the core, and the primary ɛ-amine groups of lysine in PLL to electrostatically interact with negatively charged phosphate groups of DNA to deposit with the PLA core. The physicochemical properties (morphology, particle size and surface charge) and the biological properties (protection from nuclease degradation, plasma stability, in vitro cytotoxicity, and in vitro transfection ability in HeLa and HepG2 cells) of the gene-loaded PLA-PEG-PLL nanoparticles (PLA-PEG-PLL NPs) were evaluated, respectively. Agarose gel electrophoresis assay confirmed that the PLA-PEG-PLL NPs could condense DNA thoroughly and protect DNA from nuclease degradation. Initial experiments showed that PLA-PEG-PLL NPs/DNA complexes exhibited almost no toxicity and higher gene expression (up to 21.64% in HepG2 cells and 31.63% in HeLa cells) than PEI/DNA complexes (14.01% and 24.22%). These results revealed that the biodegradable tri-block copolymer PLA-PEG-PLL might be a very attractive candidate as a non-viral vector and might alleviate the drawbacks of the conventional cationic vectors/DNA complexes for gene delivery in vivo.
    International Journal of Molecular Sciences 12/2011; 12(2):1371-88. DOI:10.3390/ijms12021371 · 2.86 Impact Factor
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    Fei Gao · Dailun Hou · Bin Zhao · Xiaoli Sun · Haitao Sun · Ning Li · Lijun Guo · Cheng Liu
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    ABSTRACT: A case-control study. To analyze the facet joint orientation in the sagittal plane [pedicle-facet angle (P-F angle)] and facet tropism, and clarify the relationship between P-F angle and the amount of vertebral slipping in degenerative spondylolisthesis (DS) patients on multislice computed tomography using multiplanar reformations techniques. Some studies have indicated a correlation between DS and an increased sagittal orientation of the facet joints. However, the facet orientation has not been fully elucidated and it had been measured only in the transverse plane of computed tomography and magnetic resonance imaging. Although the P-F angle had been measured on the plain radiographs, accurate measurement was difficult to obtain because of the technical limitations. A total of 156 patients, who came to our hospital for low back pain and/or sciatica were divided into 2 groups. The DS group comprised of 78 patients with DS at L4-L5, and the control group comprised of 78 patients without spondylolisthesis. The P-F angle and tropism were measured in the sagittal plane on multi-slice computed tomography using multiplanar reformations techniques. The P-F angles at L4-L5 were 117.02±6.89 degrees (left), 115.95±6.02 degrees (right) in the DS group and 106.71±3.19 degrees (left), 105.58±3.07 degrees (right) in the control group, respectively (P₁<0.01, P(r)<0.01). The facet tropism at L4-L5 in the DS group was significantly increased, compared with that in the control group (P=0.004). The mean P-F angle at L4-L5 did not correlate with the amount of vertebral slipping (r=0.176, P=0.122). The P-F angle was the highest at L4-L5 both in the DS and the control group, which might explain the fact that L4 vertebra is more likely to slip forward. The P-F angle of the slipped vertebra alone was more horizontally inclined and facet tropism in the sagittal plane may relate well to DS.
    Journal of spinal disorders & techniques 11/2011; 25(2):E18-22. DOI:10.1097/BSD.0b013e31823972d4 · 1.89 Impact Factor
  • Peng Li · Donghua Liu · Xiaoli Sun · Chunxi Liu · Yongjun Liu · Na Zhang
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    ABSTRACT: The clinical success of gene therapy for lung cancer is not only dependent on efficient gene carriers but also on a suitable delivery route. A pulmonary delivery route can directly deliver gene vectors to the lung which is more efficient than a systemic delivery route. For gene carriers, cationic liposomes have recently emerged as leading non-viral vectors in worldwide gene therapy clinical trials. However, cytotoxic effects or apoptosis are often observed which is mostly dependent on the cationic lipid used. Therefore, an efficient and safe cationic lipid, 6-lauroxyhexyl lysinate (LHLN), previously synthesized by our group was first used to prepare cationic liposomes. Physicochemical and biological properties of LHLN-liposomes were investigated. LHLN-liposome/DNA complexes showed positive surface charge, spherical morphology, a relatively narrow particle size distribution and strong DNA binding capability. Compared with Lipofectamine2000, the new cationic liposome formulation using LHLN exhibited not only lower cytotoxicity (P < 0.05) but also similar transfection efficiency in A549 and HepG2 lung cancer cells for in vitro tests. When administered by intratracheal instillation into rat lungs for in vivo evaluation, LHLN-liposome/DNA complexes exhibited higher pulmonary gene transfection efficiency than Lipofectamine2000/DNA complexes (P < 0.05). These results suggested that LHLN-liposomes may have great potential for efficient pulmonary gene delivery.
    Nanotechnology 06/2011; 22(24):245104. DOI:10.1088/0957-4484/22/24/245104 · 3.67 Impact Factor
  • Xiaoli Sun · Cheng Liu · Rengui Wang · Xuejun Zhu · Li Gao · Jiuhong Chen
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    ABSTRACT: Castleman's disease (CD) is an uncommon entity characterized by a massive growth of lymphoid tissue. There are two types: the hyaline-vascular (HV) type and the plasma cell (PC) type. The purpose of this study was to evaluate the clinical value of multiple detector computed tomography (MDCT) in the diagnosis and planning of treatment for hyaline-vascular CD. Fifty-two cases of confirmed hyaline-vascular CD were retrospectively reviewed. Unenhanced and contrast-enhanced MDCT scans had been performed in all patients, followed by surgery and pathological analysis of the lesion. Original MDCT transverse and reconstructed images were used for image interpretation. Features of the lesion and its adjacent structures were identified. The lesion was present in the thorax of 24 patients and the abdomen in 28. Obvious features of hyaline-vascular CD (especially feeding vessels and draining veins) and its adjacent structures were demonstrated on 52 patients. On MDCT imaging, original MDCT transverse and reconstructed images provide an excellent tool for diagnosis of hyaline-vascular CD and have high value in the determination of a treatment plan.
    European journal of radiology 06/2011; 81(9):2436-9. DOI:10.1016/j.ejrad.2011.05.024 · 2.16 Impact Factor
  • Xiaoli Sun · Na Zhang
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    ABSTRACT: The polyplexes which are formed between cationic polymers and DNA through electrostatic interactions and thus known as polycation/DNA complexes, are by far the most widely used non-viral gene delivery vectors. Many factors such as molecular weight, surface charge, charge density, hydrophilicity and the structure of cationic polymers affect gene transfection efficiency of cationic polymers. Therefore, optimization of cationic polymers is necessary to improve the gene transfection efficiency. Currently several important cationic polymers were used as cationic vectors for gene delivery which included PEI, PLL, Chitosan and PAMAM. Their most advantages and the rational design are introduced in this article. However, these systems are much less efficient in gene transfer experiments compared with viral systems. Some strategies such as PEGylation, combination and multifunctional modification were developed in the cationic polymeric vectors for gene delivery. Hereby, this article will review various kinds of copolymers with higher stability but biodegradable, bioresponsive and easy refined molecular weight which could be easily modification. Especially, the multifunctional modified polyplexes and polymersomes will be further discussion due to their ability to conjugate biologically active ligands, which can be used as potential nanostructured biomaterials for future in vivo gene delivery.
    Mini Reviews in Medicinal Chemistry 02/2010; 10(2):108-25. DOI:10.2174/138955710791185109 · 3.19 Impact Factor
  • Xiaoli Sun · Cheng Liu · Changhu Liang · Cong Sun · Shuwei Liu · Kai Deng
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    ABSTRACT: To prospectively assess the relationship between the time to peak enhancement of hepatocellular carcinomas (HCC) and that of the aorta at 64-detector computed tomography (CT). The study prospectively included 43 patients with known HCC. All underwent abdominal CT imaging by using BodyPerfusion CT model. The CT data acquisition was initiated with a delay of 8-15s from the beginning of the contrast material administered. The time-density curves (TDC) of the HCC and the aorta were drawn. The times to peak enhancement of the HCC and the aorta were recorded and the correlation between the time to peak enhancement of the HCC and that of the aorta was analyzed. There were three tendencies of TDC of the HCC enhancement, only 23.3% of them were similar to that of the aorta. The mean time to peak enhancement of the aorta and the HCC (86.1%) was 23.38 s and 30.04 s, respectively. The time to peak enhancement of most HCC was positively and linearly correlated with the time to peak aortic enhancement (r=0.662, P<0.05). The result may potentially allow scan delay optimization at contrast material-enhanced CT image in the detection of HCC according to interindividual variability.
    Computerized medical imaging and graphics: the official journal of the Computerized Medical Imaging Society 06/2009; 33(4):312-6. DOI:10.1016/j.compmedimag.2009.02.002 · 1.50 Impact Factor
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    ABSTRACT: To evaluate the value of three-dimensional (3D) constructive interference in steady-state (CISS) magnetic resonance (MR) imaging with multi-planar reconstruction (MPR) in displaying the relationship between the oculomotor nerve and its adjacent structures for patients with oculomotor paralysis. 17 consecutive patients with oculomotor paralysis were examined with 3D-CISS and conventional spin-echo (SE) sequences on a 1.5-Tesla MR system. Original transverse and MPR images were used for image interpretation. The features of the oculomotor nerve and its adjacent structures were identified. The diagnosis was surgically confirmed in all patients. Through 3D-CISS with MPR images, obvious relationship of the oculomotor nerve and its adjacent structures was demonstrated on 17 patients. Of those oculomotor nerves, 15 were compressed by the arteries (n=15), one by the craniopharyngioma (n=1), and another one by the neurofibroma (n=1). 3D-CISS MR imaging with MPR provides an excellent way to characterize the relationship between the nerve and its adjacent structures in the cisternal segment of the oculomotor nerve in the patients with oculomotor paralysis. Moreover, this method shows anatomical details for imaging diagnosis and surgical procedure.
    European journal of radiology 01/2009; 73(2):221-3. DOI:10.1016/j.ejrad.2008.11.004 · 2.16 Impact Factor
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    ABSTRACT: PURPOSE The purpose of this study was to prospectively assess the clinical value of three-dimensional constructive interference in steady state (3D-CISS) sequence at magnetic resonance (MR) and multi-planar reconstruction (MPR) technique in displaying the detailed anatomy of the cisternal segment of oculomotor nerve and its adjacent structures. METHOD AND MATERIALS Ethics committee approval and informed consent were obtained. Eighteen consecutive patients with paralysis of unilateral oculomotor nerve (11 men, 7 women; mean age, 63 years) were examined with 3D-CISS sequence and conventional spin echo (SE) sequence at 1.5-Tesla MR scanner. The cisternal segment of oculomotor nerve and its adjacent structures were observed from various planes on MPR images. The images were reviewed by two experienced radiologists. Consensus was obtained from discussion. All the cases were verified by operation. RESULTS Eighteen patients were showed clearly detailed anatomy of the cisternal segment of oculomotor nerve and the correlation with its adjacent structures on 3D-CISS MPR images. Of those, fifteen oculomotor nerves were compressed by the arteries (n=15), one by craniopharyngioma (n=1), two by the tumors in pontine cistern (n=2). CONCLUSION 3D-CISS sequence at MR and MPR technique are the optimal imaging methods in displaying the cisternal segment of oculomotor nerve and its adjacent structures. Moreover, the methods can supply anatomical details for imaging diagnosis and surgical anatomy. CLINICAL RELEVANCE/APPLICATION 3D-CISS sequence at MR and MPR technique can supply anatomical details for imaging diagnosis and surgical anatomy.
    Radiological Society of North America 2008 Scientific Assembly and Annual Meeting; 12/2008

Publication Stats

135 Citations
26.41 Total Impact Points

Institutions

  • 2011–2013
    • Capital Medical University
      Peping, Beijing, China
  • 2009–2012
    • Shandong University
      • School of Pharmaceutical Sciences
      Chi-nan-shih, Shandong Sheng, China