Joanna. Milala

Publications (11)9.02 Total impact

• Article: Content of quercetin glycosides and fructooligosaccharides in onion stored in a cold room

  Katarzyna Grzelak, Joanna Milala, Bogusław Król, Franciszek Adamicki, Ewa Badełek
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  ABSTRACT: In the paper, the authors present the qualitative and quantitative composition of quercetin glycosides and fructooligosaccharides (FOS) in onions commonly grown in Poland of the varieties: Napoleon, Sprinter and Grabowska from the 2006 and 2007 crops, stored in a cold room under standard conditions from October until February. Quercetin glycosides and FOS have been determined using the HPLC method. It has been demonstrated that the mean content of quercetin glycosides in the three varieties of onions from the 2006 and 2007 seasons is 1,381.6±123.1 and 1,479.4±125.5mg/100gd.m., respectively, in the edible part, whereby the proportion of quercetin 3-glucoside and quercetin 4′-glucoside amounts to about 60%. In 2006 in the three onion varieties the FOS content was on average 7.0±1.2g/100gd.m., while in 2007 it was 6.2±0.9g/100gd.m., of the edible part. The content of quercetin glycosides in outer and middle scales of the onions was 3,386.3±190 and 1,017.4±188mg/100gd.m., respectively. In the outer scales the FOS content was lower and amounted to 3.1±0.2g in 100gd.m., while in the middle scales it was significantly higher 6.8±0.7g/100gd.m. The content of quercetin glycosides in onions is high and remains at a constant level during storage; however, since they are found mainly in outer scales, their total removal with a view to obtaining white and milder culinary products results in a loss of approximately half of polyphenols.
  European Food Research and Technology 04/2012; 228(6):1001-1007. · 1.57 Impact Factor
• Article: Consumption of polyphenol concentrate with dietary fructo-oligosaccharides enhances cecal metabolism of quercetin glycosides in rats.

  Jerzy Juśkiewicz, Joanna Milala, Adam Jurgoński, Bogusław Król, Zenon Zduńczyk
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  ABSTRACT: We verified the hypothesis that the consumption of polyphenol concentrate (PC), rich in quercetin and its glycosides (36 g/100 g), in association with different dietary fiber matrices, that is, an easily fermentable fructo-oligosaccharides (FOS) or non-fermentable cellulose (CEL), causes a disparate adaptive response of the cecal microbial activity in rats. This in turn facilitates further utilization of biologically active polyphenolic compounds, which are not, as usual, digested in the foregut. Four-week experimental feeding of male Wistar rats consisted of diets containing 5% FOS or CEL, as a source of dietary fiber, with or without 0.3% addition of PC. Positive changes in rat cecum were observed resulting from the ingestion of an FOS-containing diet, such as decreased pH and increased the production of short-chain fatty acids in the digesta, compared with a CEL-containing diet. The addition of PC to the FOS diet did not eliminate the positive effects of the latter, except for a slight increase in cecal pH and a decrease in microbial glycolytic activity. However, a simultaneous increase in the cecal butyrate pool was also observed. An adaptation process of the microflora enzymatic system to dieting with PC and FOS was proven in further enhanced susceptibility of rutin (quercetin 3-O-glucorhamnoside), hyperoside (quercetin 3-O-galactoside), and quercitrin (quercetin 3-O-rhamnoside) to hydrolysis by the cecal digesta solution. Especially when consumed together, PC and FOS are important dietary factors affecting the susceptibility of quercetin glycosides to microbial metabolism in the cecum. The intensification of the hydrolysis of quercetin glycosides by dietary treatments leads also to the increased metabolism of quercetin itself.
  Nutrition 03/2011; 27(3):351-7. · 3.03 Impact Factor
• Article: Effect of the dietary polyphenolic fraction of chicory root, peel, seed and leaf extracts on caecal fermentation and blood parameters in rats fed diets containing prebiotic fructans.

  Jerzy Juśkiewicz, Zenon Zduńczyk, Ewa Zary-Sikorska, Bogusław Król, Joanna Milala, Adam Jurgoński
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  ABSTRACT: The aim of this 28 d experiment was to examine the physiological response of growing rats to a dietary combination of prebiotic chicory fructans, with polyphenols originating from different parts of the chicory plant, i.e. roots, root peels, seeds and leaves. A total of forty rats were assigned to groups fed the following diets characterised by a similar content of oligofructose and inulin: control, with 10 % of a root extract (a low level of dietary polyphenols, 0·05 %), with 6·5 % of a root peel extract (a medium level of dietary polyphenols, 0·107 %), with a combination of 8 % of a peel extract and 0·8 % of a seed extract (a high level of dietary polyphenols, 0·208 %) and with 2·5 % of a leaf extract (a medium level of dietary polyphenols, 0·106 %, with chicoric acid constituting half of them). Chicory seeds are the richest source of polyphenols, especially abundant in dicaffeoylquinic acids. When applied as a dietary supplement, the mixture of monocaffeoylquinic and dicaffeoylquinic acids, from the extracts made of roots, root peels and seeds, elicited more favourable changes in parameters of the antioxidative status of the body and in the activity of bacterial β-glucuronidase in the faeces and caecal digesta. In turn, the extract from chicory leaves, containing considerable quantities of chicoric acid and polyphenolic glycosides, apart from chlorogenic acids, also triggered desirable changes in the lipid profile of the blood serum. The high concentration of polyphenols in the extracts examined enables their application as dietary supplements to be administered in low doses.
  The British journal of nutrition 03/2011; 105(5):710-20. · 3.45 Impact Factor
• Article: Gastrointestinal tract metabolism of young turkeys fed diets supplemented with pure nystose or a fructooligosaccharide mixture.

  Jerzy Juśkiewicza, Zenon Zduńczyk, Jan Jankowski, Bogusław Król, Joanna Milala
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  ABSTRACT: In a four-week experiment on 60 7-day-old BUT-9 male turkeys the effects of dietary fructooligosaccharides (pure nystose and a fructooligosaccharide mixture) supplemented at 1 and 2%, were studied on ileal and caecal metabolism. The control carbohydrate was cellulose, added also at 1 or 2%. Each dietary treatment consists of 10 birds kept individually. The average degree of polymerisation of the nystose and oligofructose preparation amounted to 2.9 and 4.1, respectively. The addition of nystose significantly decreased the pH value and viscosity in the ileal contents compared with the cellulose treatment. On the other hand, the oligofructose preparation increased the activity of sucrase and lactase in the ileal mucosal by 30-60% and 33-47%, respectively. Both fructan preparations similarly acidified the caecal and colonic digesta (by 0.2-0.4 pH units) as well as diminished the activity of bacterial harmful beta-glucuronidase (by 24-40%), but only nystose caused an enlargement of the caeca and effectively reduced caecal ammonia concentration, especially at a higher dose. Oligofructose supplementation at 2% caused a 3.5-fold increase of bacterial activity of alph- and beta-galactosidase, while 2% nystose resulted in 1.7 and 3 times higher alpha- and beta-glucosidases activities, respectively. Compared to oligofructose, dietary nystose increased propionic and decreased butyric fermentation in caeca. Nystose and oligofructose preparations added at 2% reduced the triacylglycerol concentration in the serum in comparison to the addition of 2% cellulose by 46 and 25%, respectively. Beside the fact that dietary levels of supplementation were of great importance, the results indicated that even small difference in the length of carbohydrate chain may cause different physiological responses.
  Archives of animal nutrition 11/2008; 62(5):389-403. · 0.99 Impact Factor
• Article: The content of saponins in white sugar and thick juice samples from Polish sugar factories.

  Bogusław Król, Joanna. Milala, Maciej. Wojtczak
• Article: The composition of saponins from sugar beet raw juice.

  Bogusław Król, Joanna. Milala
• Article: Żelazo jako zanieczyszczenie techniczne produktów cukrowniczych.

  Maciej. Wojtczak, Joanna. Milala
• Article: Oznaczenie zawartości saponin w półproduktach i produktach cukrowniczych z zastosowaniem metody HPLC.

  Bogusław Król, Joanna. Milala
• Article: Skład saponin soku surowego.

  Bogusław Król, Joanna. Milala
• Article: Sastav i biološka svojstva etanolnih ekstrakata korijena, kore, sjemenja i listova cikorije

  a.jurgonski@pan.olsztyn.pl, Joanna. Milala, Jerzy Juśkiewicz, Zenon Zduńczyk, Bogusław Król
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  ABSTRACT: The chemical composition of the ethanol extracts of chicory root, peel, seed and leaf has been determined, in particular their inulin and phenolic fractions. The root and peel extracts were characterized by large mass fractions of inulin (60.1 and 46.8 g per 100 g of fresh mass, respectively), predominantly with degree of polymerization in the range from 3 to 10, while phenolics, determined as caffeoylquinic acids, made up 0.5 and 1.7 g per 100 g of fresh mass, respectively. The leaf and seed extracts had decidedly lower mass fractions of inulin (1.7 and 3.2 g per 100 g of fresh mass, respectively) and higher mass fractions of phenolics (9.6 and 4.22 g per 100 g of fresh mass, respectively) recognized as caffeoylquinic acids, chicoric acid and quercetin glucuronide. The biological properties of a non-inulin fraction from each extract were determined on Wistar rats fed with diets rich in fructose and saturated fat, as a model of metabolic changes related to westernization of human eating habits. The diets contained the same amount of inulin (6 %) with various phenolic fractions. Some changes were noted in the microbial enzymatic activity of the caecum after feeding for 4 weeks with the diet containing the highest mass fraction of phenolics (0.208 %), derived from the mixture of peel and seed extracts (decreased activity of β-galactosidase and β-glucuronidase), as well as with the diet containing leaf extract (decreased β-glucuronidase activity). All the diets showed no essential influence on the caecal concentration and profile of short-chain fatty acids, except acetate, whose concentration decreased significantly in rats fed with the diet enriched with root extract. The addition of peel and leaf extracts to the fructose diets significantly increased the serum antioxidant capacity of lipophilic substances. The study indicates that parts of chicory and its byproducts might be a source of valuable compounds to improve the physiological activity of inulin.
  Food Technology and Biotechnology (imacan@pbf.hr); Vol.49 No.1.
• Source

  Available from: srce.hr

  Article: Composition of Chicory Root, Peel, Seed and Leaf Ethanol Extracts and Biological Properties of Their Non-Inulin Fractions

  Adam Jurgon Bski, Joanna Milala, Jerzy Jus Bkiewicz, Zenon Zdun Bczyk, Bogus³aw Król
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  ABSTRACT: The chemical composition of the ethanol extracts of chicory root, peel, seed and leaf has been determined, in particular their inulin and phenolic fractions. The root and peel extracts were characterized by large mass fractions of inulin (60.1 and 46.8 g per 100 g of fresh mass, respectively), predominantly with degree of polymerization in the range from 3 to 10, while phenolics, determined as caffeoylquinic acids, made up 0.5 and 1.7 g per 100 g of fresh mass, respectively. The leaf and seed extracts had decidedly lower mass frac-tions of inulin (1.7 and 3.2 g per 100 g of fresh mass, respectively) and higher mass frac-tions of phenolics (9.6 and 4.22 g per 100 g of fresh mass, respectively) recognized as caffeoylquinic acids, chicoric acid and quercetin glucuronide. The biological properties of a non-inulin fraction from each extract were determined on Wistar rats fed with diets rich in fructose and saturated fat, as a model of metabolic changes related to westernization of human eating habits. The diets contained the same amount of inulin (6 %) with various phenolic fractions. Some changes were noted in the microbial enzymatic activity of the caecum after feeding for 4 weeks with the diet containing the highest mass fraction of phenolics (0.208 %), derived from the mixture of peel and seed extracts (decreased activity of b-galactosidase and b-glucuronidase), as well as with the diet containing leaf extract (decreased b-glucuronidase activity). All the diets showed no essential influence on the caecal concentration and profile of short-chain fatty acids, except acetate, whose concentra-tion decreased significantly in rats fed with the diet enriched with root extract. The addi-tion of peel and leaf extracts to the fructose diets significantly increased the serum antioxi-dant capacity of lipophilic substances. The study indicates that parts of chicory and its byproducts might be a source of valuable compounds to improve the physiological activ-ity of inulin.