Yoshihiro Murakami

Publications (3)4.48 Total impact

• Article: Efficient improved extraction of tomato saponin using shock waves.

  Hideyuki Manabe, Ayumi Takemoto, Hiromichi Maehara, Mizuho Ohno, Yoshihiro Murakami, Shigeru Itoh, Toshihiro Nohara
  [show abstract] [hide abstract]
  ABSTRACT: In the conventional method of mixer blending extraction, the yields of the tomato-saponin, esculeoside A, in the mini and middy tomatoes were found to be 0.043% and 0.046%, respectively. In order to improve the yields, we attempted a more efficient extraction using shock waves. The yields of esculeoside A were 0.0987% in air after 1 shock, 0.0792% in air after two shots, 0.0648% in half water after 1 or 2 shocks, and 0.0599% in water after 1 or 2 shocks. The yields obtained by the proposed method were approximately twice those of the conventional mixer blending method; therefore, this method is regarded to be very efficient. Moreover, two spirosolane glycosides, tomatine and lycoperoside A, were obtained for the first time from the ripe tomato fruit in this method. To date, these compounds have not been obtained with the mixer blending method. However, whether these glycosides are produced from esculeoside A or are newly extracted from the plant organ by the shock wave is still unclear.
  Chemical & pharmaceutical bulletin 01/2011; 59(11):1406-8. · 1.70 Impact Factor
• Article: Content variations of the tomato saponin esculeoside A in various processed tomatoes.

  Hideyuki Manabe, Yoshihiro Murakami, Mona El-Aasr, Tsuyoshi Ikeda, Yukio Fujiwara, Masateru Ono, Toshihiro Nohara
  [show abstract] [hide abstract]
  ABSTRACT: A tomato saponin, esculeoside A, may be metabolized into various steroidal hormones such as pregnane derivatives that are expected to exhibit various bioactivities in the body, such as anti-osteoporosis, anti-menopausal disorder and anti-tumor actions. Therefore, we attempted to examine the esculeoside A contents of fresh tomatoes, tomatoes boiled in water, tomatoes heated using a microwave oven, freeze-dried tomatoes, and commercially available processed tomato products contained in plastic bottles and cans, in order to develop a health food.
  Journal of Natural Medicines 01/2011; 65(1):176-9. · 1.39 Impact Factor
• Article: Study of the chemical constituents of Pruni Cortex and its related parts.

  Nanae Matsuoka, Tsuyoshi Ikeda, Mona El-Aasr, Hideyuki Manabe, Yoshihiro Murakami, Hiroya Deguchi, Toshihiro Nohara
  [show abstract] [hide abstract]
  ABSTRACT: The water extract of Pruni Cortex, which is obtained from the bark of Prunus jamasakura, is a major component in Brocin. We investigated the constituents of the water and methanolic extracts of Pruni Cortex. From the water extractive, two flavonoids (sakuranin and neosakuranin; major constituents of this extractive), four known lignans, and six aromatic compounds including one new compound were obtained. In addition, we also investigated the constituents of the flowers and the leaves of Prunus yedoensis.
  Journal of Natural Medicines 01/2011; 65(1):166-71. · 1.39 Impact Factor