Xiangshi Tan

Publications (1)2.1 Total impact

• Article: Spectroscopic study on acid-induced unfolding and refolding of apo-neuroglobin.

  Jianshuai Mu, Lianzhi Li, Yujing Guo, Zhanglei Qiu, Xiangshi Tan
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  ABSTRACT: pH-induced unfolding and refolding of apo-neuroglobin (apo-Ngb) were investigated by UV, fluorescence, circular dichroism (CD) spectra and light scattering measurements. Results revealed that apo-Ngb became partially unfolded at around pH 5.0, with evidences from a red shift in the fluorescence spectra, a decrease in the far-UV CD and a sharp peak in the light scattering intensity. Further lowering of the pH reversed these effects, suggesting that apo-Ngb folds back to a compact state. At pH 2.0, the apo-Ngb forms a folding intermediate known as molten globule (MG), which is possessed of native-like secondary structure and almost complete loss of tertiary structure. Based on these results, the acid-induced denaturation pathway of apo-Ngb can be illustrated from the native state (N), via a partially unfolded state (U(A)) to the molten globule state (MG).
  Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy 02/2010; 75(5):1600-4. · 2.10 Impact Factor