Takashi Setoguchi

Kagoshima University, Kagosima, Kagoshima, Japan

Are you Takashi Setoguchi?

Claim your profile

Publications (9)13.37 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The purpose of this study was to evaluate the association between oral malodour and periodontal disease, and to determine the effect of periodontal therapy on oral malodour. Oral malodour parameters, including volatile sulphur compound (VCS) measurement, methyl mercaptan/hydrogen sulphide ratio by gas chromatography, organoleptic testing, tongue coating score, and periodontal parameters were evaluated in 823 patients complaining of oral malodour. Amongst these patients, 89 with oral pathogenic halitosis received tongue cleaning and periodontal therapy. Oral malodour and periodontal parameters were measured at baseline and after treatment. Amongst 823 patients, 102 were diagnosed with gingivitis and 721 with periodontitis. VCS levels and periodontal parameters increased according to the severity of oral malodour. Organoleptic testing significantly correlated with periodontal probing depth and a percentage of periodontal pocket depth ≥4mm (r=0.40 and 0.39 respectively). There were significant correlations between methyl mercaptan/hydrogen sulphide ratio and periodontal parameters. Significant decrease in oral malodour and periodontal parameters in 89 patients with oral pathogenic halitosis was also observed after periodontal treatment. Oral malodour is associated with periodontal disease, and periodontal therapy combined with tongue cleaning is beneficial for oral pathogenic halitosis.
    Oral Diseases 10/2010; 16(7):702-6. · 2.38 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Regeneration of lost periodontium is the ultimate goal of periodontal therapy. Bone grafts, guided tissue regeneration, and application of growth factors are used for periodontal regeneration. This study aimed to evaluate the clinical efficacy of a new, injectable calcium phosphate bone cement (CPC) in human periodontal intrabony defects. Thirty subjects (mean age, 53.4 +/- 9.1 years) with periodontitis and narrow intrabony defects were enrolled in the study. Subjects were classified randomly into the CPC graft group (N = 15) or the open flap debridement (OFD) alone group (N = 15). Clinical measurements were performed at baseline and at 3, 6, 9, and 12 months; radiographs were taken at baseline, 2 weeks, and 6 and 12 months after surgery. The Student t test was used for statistical analysis. In the CPC group, six cases showed exposure or loss of the CPC within 12 months, whereas the remaining nine cases (CPC-R group) showed no adverse reaction, including infection or suppuration. Overall, CPC-R and OFD treatment groups exhibited a significant reduction in probing depth and a significant gain in clinical attachment level at 3, 6, 9, and 12 months compared to baseline values. However, there were no significant differences in any of the clinical parameters between the groups. In the CPC-R group, radiographic bone level gain appeared to be greater than in the OFD group. The present study failed to demonstrate any superior clinical outcomes for the CPC group compared to the OFD group; however, radiographs revealed more favorable results in the CPC-R group. The filling volume and stiffness of CPC may compromise the clinical outcomes for periodontal intrabony defects.
    Journal of Periodontology 02/2008; 79(1):25-32. · 2.40 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Volatile sulfur compounds such as hydrogen sulfide (H(2)S) and methyl mercaptan (CH(3)SH) are the main causes of oral mal odor. However, the physiological functions of H(2)S have not been investigated in oral tissues. The aim of this study was to evaluate the effect of H(2)S on cell proliferation and the cell cycle in oral epithelial-like cells. Ca9-22 cells were used in this study. Cells were cultured in 5% CO(2)/95% air with (5 or 10 ng/mL) or without H(2)S. DNA synthesis was measured using a 5-bromo-2-deoxyuridine enzyme-linked immunosorbent assay. The cell cycle was analyzed using a flow cytometer. The expressions of phosphorylated retinoblastoma protein (Rb), p21(Cip1) and p27(Kip1) were evaluated by western blotting. Exposure to 5 and 10 ng/mL of H(2)S significantly decreased DNA synthesis (p < 0.05). Cell cycle analysis also showed that exposure to both concentrations of H(2)S significantly increased the proportion of cells in G(1) phase (p < 0.001) and significantly decreased the proportion of cells in S phase (p < 0.01). Western blotting showed that Rb phosphorylation was reduced and p21(Cip1) was enhanced by exposure to H(2)S. The results indicated that H(2)S inhibits cell proliferation and induces cell cycle arrest via the expression of p21(Cip1) in Ca9-22 cells.
    Journal of Periodontal Research 02/2008; 43(1):90-5. · 1.99 Impact Factor
  • Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) 01/2006; 48(3):174-181.
  • Periodontology 2000 02/2004; 36:45-58. · 4.01 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Hepatocyte growth factor (HGF), also known as scatter factor, is a broad-spectrum and multifunctional cytokine required for the development, growth and regeneration of various organs and tissues. The expression of HGF in human gingival fibroblasts is induced by inflammatory cytokines such as interleukin 1. Thus, although it is possible that content of HGF in gingival crevicular fluid (GCF) in periodontitis is increased, this has not so far been reported because the volume of GCF is too small to determine HGF by the available enzyme-linked immunosorbent assay (ELISA). A recently developed, highly sensitive ELISA for HGF, with a detection limit of 1 pg/ml sample, has now enabled HGF to be measured in GCF.The mean HGF content in GCF from sites with clinically healthy gingiva, defined by the absence of overt signs of gingival inflammation and a probing depth (PD) <3 mm, was 1.7 ng/ml, and that of periodontitis, defined by obvious alveolar bone loss detected by radiographic examination and a PD> or =3 mm, was 3.23 ng/ml. Although treating the periodontitis did not significantly decrease the HGF concentration despite significantly improved clinical scores such as PD and Gingival Index, the total amount of HGF in GCF did decrease significantly after treatment. HGF was expressed by gingival fibroblasts and inflammatory cells as determined by in situ hybridization. HGF-activator (HGFA), which converts inactive pro-HGF to active mature HGF, was detected in gingival epithelial cells by immunostaining. The expression of HGFA was also confirmed in gingival tissue by reverse transcription-polymerase chain reaction (RT-PCR). These findings indicate that HGF is synthesized and activated in gingiva that is clinically healthy or associated with periodontitis.
    Archives of Oral Biology 10/2002; 47(9):655-63. · 1.55 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Previous studies have demonstrated that methyl mercaptan (CH3SH), one of the main causes of oral malodour, might contribute to the initiation and progression of periodontal disease. These studies suggested that CH3SH may affect the epithelial cells of the gingival crevice, which form a barrier to the penetration of microbial substances. In this study, the effects of CH3SH on the epithelial cells and gingival fibroblasts were investigated. Human oral epithelial carcinoma cell line (KB), human oral squamous cell carcinoma cell line (HSC-2), and human gingival fibroblasts (HGF) derived from healthy gingiva were used in this study. These cells were cultured in conditions of 5% CO2/95% air with or without CH3SH (10 ng/ml or 50 ng/ml) for 5 days. Cell numbers, proliferation and cytotoxicity were evaluated. CH3SH inhibited epithelial cell growth and proliferation at the concentration of 50 ng/ml, and a cytotoxic effect of CH3SH was also noted. On the other hand, HGF cells were not affected by 50 ng/ml CH3SH. High concentrations of CH3SH such as 50 ng/ml have an inhibitory effect on the growth and proliferation of epithelial cells, but not on those of fibroblasts.
    International Dental Journal 07/2002; 52 Suppl 3:241-6. · 1.04 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This clinical study was performed to evaluate the clinical and radiographical outcomes of a ready-to-use EMDOGAIN^[○!R]-gel. Twenty one periodontitis patients (33 sites) with infrabony defects were selected. After initial treatment, clinical parameters such as probing depth (PD), clinical attachment level (CAL), bleeding on probing, tooth mobility and gingival recession were recorded and radiographs for bone level measurement were taken at baseline. Examinations were repeated 8 and 18 months post-operatively. The mean PD was 7.68 mm at baseline, 3.69 mm at 8 months, and 3.70 mm at 18 months. The mean gain of CAL was 2.50 mm at 8 months and 2.70 mm at 18 months, and the radiographic measurements showed a mean bone level gain of 1.17 mm at 8 months and 1.69 mm at 18 months. This series of cases demonstrated a statistically significant reduction of PD, gain of CAL and bone level at 8 months and 18 months.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Recently, the persons concerning about halitosis are increasing. For special diagnosis and treatment for halitosis, Kagoshima University Medical and Dental Hospital established Oral Malodor Clinic in 1997. Over 800 patients visited the clinic in 8 years. In this review, the diagnosis and treatment system of halitosis in our clinic is discribed. The oral malodor was estimated by the analysis of the volatile sulfur compounds (VSC) level by gas chromatograph and the organoleptic analysis. The interview using the questionnaire and oral examination was also used for diagnosis. The 801 patients (278 male and 523 female) were classified into physiologic halitosis (n=147: 18.4%) and oral pathologic halitosis (n=332: 41.5%), extraoral pathologic halitosis (n=41: 5.1%), pseudo-halitosis (n=254: 31.7%), and halitophobia (n=22: 2.8%). The treatment was done according to the treatment needs for breath malodor. In the treatment of halitosis, oral hygiene including tongue cleaning is the most important. For the patients with periodontal disease, periodontal therapy is also needed. In patients with no or less halitosis, some patients disagree with the results. For such patients, repeated examinations are needed. Extraoral pathologic halitosis or halitophobic pateients need to be referred to a specialist. For the treatment of halitosis, accurate diagnosis is important.