G Zhang

Binzhou Medical University, Pei-chen, Shandong Sheng, China

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Publications (94)204.65 Total impact

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    ABSTRACT: ObjectivesmiR-99a has been reported to function as a tumour suppressor in breast cancer. However, its role in the regulation of breast cancer stem cell (CSC) phenotype has up to now remained unknown.Materials and methodsIn this study, we isolated the side population (SP) cells by staining cultured MCF-7 and MDA-MB-231 cells with fluorescent DNA-binding dye Hoechst 33342, then by flow cytometric sorting. Next, we detected expression of miR-99a in the SP cells compared to non-SP cells using real-time PCR, and explored effects of miR-99a on the CSC phenotype of the breast cancer cells, including sphere formation, self-renewal, tumourigenicity and cell migratory capability.ResultsWe found that expression of miR-99a was down-regulated in the SP cells compared to non-SP cells. Restoration of expression of miR-99a inhibited cell migration and invasion, reduced sphere formation of breast SP cells in vitro, and suppressed tumour growth in vivo. Finally, bioinformatic prediction suggested the oncogene, mammalian target of rapamycin (mTOR) – a downstream effector of the PI3K/AKT signalling pathway, was a target gene of miR-99a in SP cells. Further, quantitative RT-PCR and western blot assays identified that overexpression of miR-99a suppressed expression of mTOR and its downstream gene, HIF-1α.Conclusion Collectively, these data suggest that miR-99a reversed the breast cancer malignant CSC phenotype, probably by targeting the mTOR signalling pathway.
    Cell Proliferation 10/2014; · 2.27 Impact Factor
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    Dataset: pjszal45
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    Dataset: H-01
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    ABSTRACT: The fluorescence spectrum of the Nd:Sc2SiO5 (Nd:SSO) crystal is measured. The central wavelength of the fluorescence band is 1080.1 nm with a full width at half-maximum (FWHM) of 3.4 nm. By using a single-walled carbon nanotube saturable absorber as a passive Q-switcher, a diode-pumped passively Q-switched intracavity frequency doubling Nd:Sc2SiO5/KTP green laser operating at 540 nm is demonstrated for the first time. The maximum average output power is 0.89 W at an incident pump power of 11.8 W, corresponding to an optical conversion efficiency of 7.5% and a slope efficiency of 10.2%. At the same incident pump power, a minimum pulse width of 94.8 ns and a maximum peak power of 19.1 W can be obtained.
    Laser Physics Letters 04/2013; 10(5):055807. · 7.71 Impact Factor
  • M Li, L Yang, H Shi, B Guo, X Dai, Z Yao, G Zhang
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    ABSTRACT: BACKGROUND: Punctate palmoplantar keratoderma (PPPK, OMIM 148600) is a rare hereditary disorder characterized by multiple punctate keratoses on the palms and soles. Recently, mutations in the alpha- and gamma-adaptin-binding protein p34 (AAGAB) and collagen, type XIV, alpha 1(COL14A1) PPPK have been reported to cause PPPK. OBJECTIVES: To identify mutations in the AAGAB and COL14A1 genes in three Chinese families with PPPK. METHODS: Genomic DNAs of the family members and 100 healthy individuals were isolated. Direct sequencing of all PCR products of the whole coding regions of AAGAB and COL14A1 genes was performed to identify the mutation. RESULTS: A heterozygous nonsense mutation c.61C>T (Q21X) was identified in AAGAB gene in two Chinese families with PPPK. We failed to detect any mutations in the AAGAB or COL14A1 gene in Family 3. CONCLUSIONS: A novel loss-of-function mutation within the AAGAB gene associated with PPPK was identified from 2 Chinese pedigrees.
    British Journal of Dermatology 02/2013; · 3.76 Impact Factor
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    ABSTRACT: BACKGROUND: Neonatal Bacillus Calmette-Guérin (BCG) vaccination induces vigorous T-helper type 1 (Th1) responses and inhibits allergy-related airway dysfunction, but the exact mechanisms remain unclear. The objective of this study was to address where the Th1 cells induced by neonatal BCG vaccination are generated and stored, and how they are recruited into the inflamed airway for the prevention of allergen-induced airway inflammation. METHODS: We vaccinated neonatal C57BL/6 mice with BCG in a mouse model of asthma and analyzed the expression and function of Th1 cells in vivo and in vitro. RESULTS: BCG vaccination-induced Th1 cells in the local inguinal lymph nodes (ILN) migrated into the lungs upon inhaled ovalbumin (OVA) challenge in OVA-sensitized mice. These CD4(+) T cells in the ILN exhibited potentials of activation, proliferation and cytokine secretion and expressed high levels of CXCR3. Adoptive transfer of CD4(+) T cells from BCG-treated ILN significantly decreased allergic airway responses. In addition, the protective effect of BCG vaccination against allergic airway inflammation was lost upon the excision of the ILN. CONCLUSIONS: These data demonstrate that ILN serves as a 'weapon' pool of Th1 cells following BCG vaccination, and these cells are ready for the migration into the inflamed lungs upon the allergen challenge, thereby inhibiting allergen-induced airway disorder.
    Allergy 01/2013; · 5.88 Impact Factor
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    ABSTRACT: BACKGROUND: Ichthyosis vulgaris (IV; OMIM 146700) is a very common inherited skin disorder. Loss-of-function mutations in filaggrin gene (FLG) have been identified as the cause of IV. In the previous study, we found that the percentage of FLG null mutations in atopic dermatitis (AD) associated IV was lower than that in isolated IV. We speculated that some clinical manifestation of IV in AD patients was not induced by FLG mutations. OBJECTIVES: In order to clarify this issue, we collected 21 IV pedigrees, 33 sporadic isolated IV patients and 116 AD associated IV patients to analyze FLG mutation frequency and filaggrin expression in isolated IV and AD associated IV. METHODS: A comprehensive sequencing of the FLG gene in all patients was performed using an overlapping PCR strategy. We also studied profilaggrin ⁄filaggrin protein expression in the skin by immunohistochemistry and measured the mRNA expression by real-time PCR in seven patients including one patient with IV harboring c.3321delA, two patients with AD associated IV harboring c.3321delA and c.6834del5 and four AD associated IV patients without FLG mutations. RESULTS: The mutation percentage of FLG gene is 74.1% and 43.1% in isolated IV patients and AD associated IV patients. Immunohistochemical staining revealed that profilaggrin⁄filaggrin peptides were remarkably reduced in the all patients' epidermis. All patients with either AD or IV showed less FLG mRNA expression compared to the normal control. CONCLUSIONS: These results indicated that factors other than FLG gene mutations can down-regulate profilaggrin ⁄filaggrin expression, leading to the ichthyosiform phenotype in the context of AD.
    British Journal of Dermatology 01/2013; · 3.76 Impact Factor
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    ABSTRACT: GAB2 is a scaffold protein with diverse upstream and downstream effectors. MAPK and PI3K signaling pathways are known effectors of GAB2. It is amplified and overexpressed in a variety of human tumors including melanoma. Here we show a previously undescribed role for GAB2 in NRAS-driven melanoma. Specifically, we found that GAB2 is co-expressed with mutant NRAS in melanoma cell lines and tumor samples and its expression correlated with metastatic potential. Co-expression of GAB2(WT) and NRAS(G12D) in melanocytes and in melanoma cells increased anchorage-independent growth by providing GAB2-expressing cells a survival advantage through upregulation of BCL-2 family of anti-apoptotic factors. Of note, collaboration of GAB2 with mutant NRAS enhanced tumorigenesis in vivo and led to an increased vessel density with strong CD34 and VEGFR2 activity. We found that GAB2 facilitiated an angiogenic switch by upregulating HIF-1α and VEGF levels. This angiogenic response was significantly suppressed with the MEK inhibitor PD325901. These data suggest that GAB2-mediated signaling cascades collaborate with NRAS-driven downstream activation for conferring an aggressive phenotype in melanoma. Second, we show that GAB2/NRAS signaling axis is non-linear and non-redundant in melanocytes and melanoma, and thus are acting independent of each other. Finally, we establish a link between GAB2 and angiogenesis in melanoma for the first time. In conclusion, our findings provide evidence that GAB2 is a novel regulator of tumor angiogenesis in NRAS-driven melanoma through regulation of HIF-1α and VEGF expressions mediated by RAS-RAF-MEK-ERK signaling.Oncogene advance online publication, 27 August 2012; doi:10.1038/onc.2012.367.
    Oncogene 08/2012; · 8.56 Impact Factor
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    ABSTRACT: By simultaneously using an electro-optic (EO) modulator and a single-walled carbon nanotube saturable absorber (SWCNT-SA) in the cavity, a diode-pumped doubly Q-switched Nd:Lu0.33Y0.37Gd0.3VO4 (Nd:LuYGdVO4) laser is demonstrated. At the incident pump power 11.43 W and f=2 kHz, the minimum pulse width 17.6 ns and the maximum pulse peak power 19,886 W can be obtained. The experimental results show that this doubly Q-switched Nd:LuYGdVO4 laser can generate shorter pulse width and higher peak power compared to the singly Q-switched Nd:LuYGdVO4 laser with only EO or SWCNT-SA.
    Optics & Laser Technology 06/2012; 44(4):903–906. · 1.37 Impact Factor
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    ABSTRACT: Diode pumped continuous wave operation and passively Q-switched performances of a Nd:Sc2SiO5 laser crystal at 1.08 microns have been demonstrated. A maximum continuous wave average output power up to 1.86 W has been obtained, corresponding to an optical conversion efficiency of 20.7% and a slope efficiency of 30.4% with respect to the absorbed pump power. With a Cr4+:YAG wafer used as the saturable absorber, a passively Q-switched Nd:Sc2SiO5 laser generated a pulse as short as 22.5 ns at a repetition rate of 20 kHz. The maximum pulse energy of 44.8 μJ was achieved, corresponding to a maximum pulse peak power of 1.66 kW. (© 2012 by Astro Ltd., Published exclusively by WILEY-VCH Verlag GmbH & Co. KGaA) (© 2011 by Astro Ltd., Published exclusively by WILEY-VCH Verlag GmbH & Co. KGaA)
    Laser Physics Letters 11/2011; 9(1):10. · 7.71 Impact Factor
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    ABSTRACT: By using a-cut Nd:Lu0.15Y0.85VO4 mixed crystal as laser gain medium, a diode-pumped passively Q-switched and mode-locked (QML) laser with a GaAs saturable absorber in a Z-type folded cavity is demonstrated for the first time. The Q-switched mode-locked laser pulses with about 90% modulation depth are obtained as long as the pump power reached the oscillation threshold. The repetition rate of the passively Q-switched pulse envelope ranges from 50 to 186 kHz as the pump power increases from 0.915 to 6.520 W. Under an incident pump power of 6.52 W, the QML pulses with the largest average output power of 694 mW, the shortest pulse width of 200 ns and the highest pulse energy of 3.73 μJ are obtained. The mode-locked pulse width inside the Q-switched envelope is estimated to be about 275 ps. The experimental results show that Nd:Lu0.15Y0.85VO4 is a promising mixed crystal for QML laser.
    Laser Physics 10/2011; 21(10):1769-1774. · 2.55 Impact Factor
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    ABSTRACT: The laser performance of a composite crystal bonded with three Nd:YVO4 single crystals has been investigated for the first time as far as we know. The largest continuous wave output power of 2.68 W is obtained at the incident pump power of 6.2 W, giving an optical conversion efficiency of 43.1% and a slope efficiency of 45.9%. For passively Q-switched operation with Cr4+:YAG of 71% initial transmission, the shortest pulse width of 18.2 ns, the largest single-pulse energy of 19.9 μJ, and the highest peak power of 1.12 kW are achieved, with the pulse repetition rate being 44.9 kHz, at the incident pump power of 6.2 W. The composite crystal can generate more excellent laser performance, when compared with the single crystals.
    Laser Physics 07/2011; 21(7):1151-1154. · 2.55 Impact Factor
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    ABSTRACT: A diode-pumped passively Q-switched Nd:Lu0.33Y0.37Gd0.3VO4 (Nd:LuYGdVO4) laser at 1.06 µm with a single-walled carbon nanotube saturable absorber is demonstrated. At the incident pump power 9.1 W, the minimum pulse width of 52 ns and the maximum peak power 66.5 W can be obtained. In order to compare different gain media, the passively Q-switched Nd:LuVO4 and Nd:Lu0.15Y0.85VO4 (Nd:LuYVO4) lasers under the same laser cavity are also investigated. The experimental results show that Nd:LuYGdVO4 crystal is more suitable for passively Q-switched laser with single-walled carbon nanotube saturable absorber than Nd:LuVO4 or Nd:LuYVO4 crystals. (© 2011 by Astro Ltd., Published exclusively by WILEY-VCH Verlag GmbH & Co. KGaA) (© 2011 by Astro Ltd., Published exclusively by WILEY-VCH Verlag GmbH & Co. KGaA)
    Laser Physics Letters 04/2011; 8(6):418 - 422. · 7.71 Impact Factor
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    ABSTRACT: A diode-pumped dual-loss-modulated Q-switched and mode-locked (QML) Nd:Lu0.15Y0.85VO4 laser with acousto-optic (AO) modulator and Cr4+:YAG saturable absorber is presented. The stable QML laser pulse with high peak power and complete modulation depth has been obtained. The QML laser characteristics such as the pulse width, single-pulse energy etc. have been measured for different small-signal transmissions (T 0) of Cr4+:YAG, different reflectivity (R) of output coupler and modulation frequencies of the AO modulator (f p ). The results show that the pulse energy increases with decreasing f p and increasing T 0, while the pulse width decreases with decreasing f p and increasing T 0. At f p = 10 kHz, R = 90%, and T 0 = 91%, the highest pulse energy and peak power of mode-locked pulses is obtained.
    Laser Physics 01/2011; 21(9):1539-1544. · 2.55 Impact Factor
  • C. Xu, G. Li, S. Zhao, X. Li, K. Cheng, G. Zhang, T. Li
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    ABSTRACT: Performance comparisons of laser-diode pumped passively Q-switched intracavity-frequency-doubled Nd:Gd0.19Y0.81VO4 and Nd:Gd0.83Y0.17VO4 lasers at 671 nm are demonstrated for the first time to our knowledge. KTP crystal is used as the frequency doubling material and V:YAG crystal as the saturable absorber with initial transmission of 89%. The dependences of average output power, pulse width, pulse repetition rate, single-pulse energy and peak power on incident pump power are measured and contrasted. The experimental results show that, Nd:Gd0.83Y0.17VO4 laser has more excellent properties than Nd:Gd0.19Y0.81VO4 laser at 671 nm.
    Laser Physics 01/2011; 21(1):101-104. · 2.55 Impact Factor
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    ABSTRACT: A diode-pumped passively Q-switched Nd:Lu0.15Y0.85VO4 laser with a GaAs output coupler is demonstrated. By using a mixed crystal Nd:Lu0.15Y0.85VO4 as laser medium, the passively Q-switched laser can generate shorter pulse width with higher peak power in comparison with the passively Q-switched Nd:LuVO4 or Nd:YVO4 lasers under the same laser cavity. At the incident pump power 11.9 W, the minimum pulse width of 3.23 ns and the maximum peak power 1.67 kW can be obtained. The average output power and the pulse repetition rate of the laser are also measured. The experimental results show that the mixed crystal is a promising laser medium for shorter Q-switched pulse with higher peak power.
    Laser Physics 01/2011; 21(1):74-78. · 2.55 Impact Factor
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    ABSTRACT: Performance comparisons of laser-diode pumped passively Q-switched intracavity-frequency-doubled Nd:Gd0.19Y0.81VO4 and Nd:Gd0.83Y0.17VO4 lasers at 671 nm are demonstrated for the first time to our knowledge. KTP crystal is used as the frequency doubling material and V:YAG crystal as the saturable absorber with initial transmission of 89%. The dependences of average output power, pulse width, pulse repetition rate, single-pulse energy and peak power on incident pump power are measured and contrasted. The experimental results show that, Nd:Gd0.83Y0.17VO4 laser has more excellent properties than Nd:Gd0.19Y0.81VO4 laser at 671 nm.
    Laser Physics - LASER PHYS. 01/2011; 21(1):101-104.
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    ABSTRACT: Two compact fiber lasers based on the gain medium of Nd-doped phosphate glass multicore fiber (MCF), of length no more than 10 cm, have been demonstrated experimentally for the first time. A stable phase-locked laser beam at 1055 nm emitted from a three-core fiber that is 8.5 cm in length has been achieved, with a maximum output power of 20 mW. The properties of similar seven-core fiber laser also have been investigated.
    Laser Physics 01/2011; 21(2):410-413. · 2.55 Impact Factor
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    ABSTRACT: By considering the Gaussian transversal and longitudinal distributions of the intracavity photon density as well as the walk-off effect of KTP crystal, the coupled rate equations of the doubly Q-switched intracavity-frequency-doubling Nd:LuVO4/KTP green laser with acoustic-optic (AO) modulator and GaAs saturable absorber are given. These equations are solved numerically and the doubly Q-switched green laser characteristics, such as the pulse width and the pulse symmetry, have been obtained. In the experiment, a diode-pumped doubly Q-switched intracavity-frequency-doubling Nd:LuVO4/KTP green laser with AO and GaAs is presented. This doubly Q-switched green laser can generate the almost absolutely symmetric pulse profile with the shorter pulse width and the higher power than the singly Q-switched green laser. The experimental results are in agreement with the theoretically numerical calculations.
    Optics Communications 07/2010; 283(14):2876–2880. · 1.44 Impact Factor
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    ABSTRACT: The monoclonal antibodies (mAbs) against ractopamine (Rac) were prepared and their properties identified by indirect competitive enzyme-linked immunoabsorbant assay (ELISA). The IC(50) of mAbs was 2.7 ng ml(-1) towards Rac or 9.3 ng ml(-1) towards Rac-glucuronides and no cross-reactivity (CR) towards other competitors except dobutamine (CR: 3.76%). Based on the mAbs, the Rac-kit (kit) and Rac-strip (strip) were developed to detect Rac residues in swine urine. The strip and kit assay could be performed within 5-10 min and 2 h, respectively, allowing the analysis of urine samples without the need for sample clean-up. The detection limits were 1 ng ml(-1) for kit and 3 ng ml(-1) with the unaided eye, and 0.2 ng ml(-1) with the Strip Reader for strip. The correlation coefficients (R(2)) were 0.988 for kit in the range 0-128.0 ng ml(-1), and 0.987 for strip in the range 0-10.8 ng ml(-1). Comparing the gas chromatography-mass spectrometry (GC-MS) with the kit or strip in swine urine spiked with Rac standards, the differences ranged from 1.4% to 4.5% for kit and 1.0% to 4.7% for strip. However, the differences were greater than 54% for the kit and 55% for the strip test for the analysis of urine from swine treated with Rac. The results obtained from GC-MS using hydrolysed urine samples were generally in good agreement with those obtained from strip or kit using non-hydrolysed urine samples.
    Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment 05/2010; 27(8):1096-103.

Publication Stats

980 Citations
204.65 Total Impact Points

Institutions

  • 2014
    • Binzhou Medical University
      Pei-chen, Shandong Sheng, China
  • 2013
    • Shanghai Dermatology Hospital
      Shanghai, Shanghai Shi, China
  • 2010–2013
    • Shandong University
      • School of Information Science and Engineering
      Chi-nan-shih, Shandong Sheng, China
  • 2012
    • Wistar Institute
      • Melanoma Research Center
      Philadelphia, Pennsylvania, United States
  • 2009–2011
    • Northeast Institute of Geography and Agroecology
      • • Graduate School
      • • Institute of Oceanology
      Beijing, Beijing Shi, China
  • 2005–2011
    • Chinese Academy of Sciences
      • • Anhui Institute of Optics and Fine Mechanics
      • • Cold and Arid Regions Environmental and Engineering Research Institute
      Peping, Beijing, China
  • 2008
    • Henan Institute of Science and Technology
      Honanfu, Henan Sheng, China
  • 2001–2003
    • Shanghai Nuclear Engineering Research and Design Institute
      Shanghai, Shanghai Shi, China
  • 2000–2003
    • Nankai University
      • Institute of Modern Optics (IMO)
      Tianjin, Tianjin Shi, China
    • Tianjin Medical University
      • Department of Radiology
      Harbin, Heilongjiang Sheng, China
  • 2002
    • Peking University
      • School of Physics
      Beijing, Beijing Shi, China
    • Zhejiang University
      • Department of Agronomy
      Hangzhou, Zhejiang Sheng, China
  • 1998
    • Beijing Medical University
      Peping, Beijing, China
  • 1996–1998
    • Clontech Laboratories, Inc.
      Mountain View, California, United States
    • Howard Hughes Medical Institute
      Ashburn, Virginia, United States
  • 1996–1997
    • FX Palo Alto Laboratory
      Palo Alto, California, United States