[Show abstract][Hide abstract] ABSTRACT: A facultatively anaerobic nitrogen-fixing bacterial strain C7(T) was isolated from a permafrost cryopeg on the Yamal Peninsula, Russia. Comparative analysis of 16S rRNA gene sequences revealed that this bacterium was closely related to Celerinatantimonas diazotrophica with the 95.5 % similarity level. Strain C7(T) differed from the species C. diazotrophica in its ability to hydrolyze gelatin and inability to use D-mannose, mellibiose, L-rhamnose, m-inositol, lactose, lactulose, D-mannitol, methyl β-D-glucoside, trehalose, acetate, DL-lactate, glycogen, cyclodextrin and L-proline as sole carbon sources. In addition, strain C7(T) grew in a temperature range of 0-34°C with optimum 18-22°C. Whole-cell fatty acid profile included C16:0, C16:1ω7, C18:1ω7, C17cyc and feature 2 comprising C12:0ald and/or unknown fatty acid 10.913 (MIDI designation) and/or iso-C16:1 /C14:0 3OH were predominant. The DNA G+C content was 44.7 mol%. The strain C7(T) (=VKM B-2511(T)=DSM 21888(T)) was assigned to a new species, for which the name Celerinatantimonas yamalensis sp. nov. was proposed, and was suggested as the type strain.
International Journal of Systematic and Evolutionary Microbiology 07/2013; · 2.79 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: A mesophilic, non-motile, hydrogenotrophic, rod-shaped methanogen, designated M2(T), was isolated from Holocene permafrost sediments of the Kolyma lowland in the Russian Arctic. Cells were 3-6 μm long and 0.45-0.5 μm wide. Strain M2(T) grew on H(2)/CO(2) and formate. Optimum conditions for growth were 37°C, pH 6.8-7.2 and 0.1 M NaCl. The DNA G+C content was 38.1 mol%. On the basis of 16S rRNA gene sequence comparison with known methanogens, strain M2(T) was affiliated with the genus Methanobacterium and was most closely related to Methanobacterium veterum MK4(T) and Methanobacterium bryantii DSM 863(T) (both 99 % 16S rRNA gene sequence similarity). However, no significant DNA-DNA relatedness was observed between strain M2(T) and these type strains. We propose that strain M2(T) represents a novel species, with the name Methanobacterium arcticum sp. nov., with type strain M2(T) (=DSM 19844(T) =VKM B-2371(T)).
International Journal of Systematic and Evolutionary Microbiology 02/2010; 61(Pt 1):144-7. · 2.79 Impact Factor