N. Khan

University of Lahore, Lāhaur, Punjab, Pakistan

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Publications (19)8.41 Total impact

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    ABSTRACT: The lesser mouse-tailed bat Rhinopoma hardwickii Gray, 1831 has not been recorded from Pakistan except from northern Punjab (Rohtas) and southern Sindh (Karachi, Landhi and Karchat Hills near Hyderabad). These records date back to 1927. Here after 84 years, we provide an account of the third country and first record of this species from southern Punjab. Rhinopomatid bats (n =5) were captured from this region of which two were euthanized to describe their external body, cranial and bacular measurements. This paper compares various morphometeric measurements of these bats to those already mentioned in literatures and documents habitat and new distribution map of this species in Pakistan.
    Journal of Animal and Plant Sciences 07/2012; 22(2):278-282. · 0.64 Impact Factor
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    Journal of Animal and Plant Sciences 04/2012; 22(4):919-922. · 0.64 Impact Factor
  • Pakistan Veterinary Journal 01/2012; 32(3):349-353. · 1.37 Impact Factor
  • Pakistan Veterinary Journal 01/2012; 32(3):349-353. · 1.37 Impact Factor
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    ABSTRACT: Fish meal is considered a major source of protein commonly used in domestic animal feeds as well as in commercial aqua feeds. The present study documents the different species used for the production of fish meal in Pakistan and to determine the proximate composition of fish meal samples obtained from these units. Samples of fish meal were collected from nine different commercial fish meal processing units and were processed for proximate analysis. Results of the proximate analysis revealed more than 60% crude protein (CP) in the fish meal samples obtained from Abdul Baqi, Shamim, Abdul Rasheed and Liaqat fish meal processing units while samples collected from rest of the processing units contained less than 60% CP. Crude fat ranged from 9.9% to 29.5%, ash content 12.7 to 28.2% and gross energy 4,118 to 4,883cal/g. The present study is a preliminary step to identify the source of fish meal production and its chemical evaluation determining the quality and its possible utilization in aqua feed production.
    Journal of Veterinary and Animal Science. 01/2012; 2:65-71.
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    ABSTRACT: In order to verify the ethnopharmacological effects of local plant, Sauromatum venosum (Ait.) Schott., on scientific lines the antibacterial activity including MIC and antioxidant activity of the crude extracts of its fruits were tested against Gram-positive and Gram-negative bacteria using well diffusion method and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity test. The results indicated a reasonable antibacterial potential and significant total antioxidant activity, thus supporting its traditional medicinal practices.
    Pakistan Journal of Botany. 01/2011; 43:579-586.
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    ABSTRACT: National Parks are diverse habitat for different medicinal plants. So far 21 areas in Pakistan have been declared as National Parks (NP). The present investigations focus on the ethnobotanical study of important medicinal plants of Chitral Gol National Park. The local inhabitants including ethnic groups of Kalash living in the vicinity of the Park have always used the medicinal herbs for various ailments and are dependent on the plants in their surrounding for, food, shelter, health, medicine, fodder and various cultural purposes. A total of 31 trees, herbs and shrubs belonging to 21 families were recorded which are used as fuel, fence and as medicine by the local inhabitants. About 100 informants and local Hakims were interviewed in this regard. Artemisia maritima, Artemisia brevifolia and Rosa webbiana are dominant species suitable for harvesting, while Ephedra gerardiana and Ferula narthex are vulnerable to harvesting. The precious ethnobotanical knowledge is disappearing very fast, so this study could be helpful in conservation of ethnobotanical knowledge.
    Pakistan Journal of Botany. 01/2011; 43:797-809.
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    ABSTRACT: In the present study a novel protocol for the removal of Albumin and Globulin in 10 genotypes of Pisum sativum were developed by using Osborn solubility class protein extraction buffer (10mM Tris-Hcl buffer, pH 6.8, 0.5 M NaCl). One major allocation of protein band and one minor protein band corresponding to 10 kda and 22 kda, respectively, was detected in SDS-PAGE (untreated cases). There is a need to further improve this method so that the minor groups of albumin and globulin could also be investigated further to characterize through 2D gel electrophoresis.
    Pakistan Journal of Botany 01/2011; 43(3):1733-1734. · 1.21 Impact Factor
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    ABSTRACT: Contamination of heavy metals is one of the major threats to water and soil as well as human health. Phytoremediation has been used to remediate metal-contaminated sites. This study evaluated the potential of 23 plant species growing on contaminated sites in Industrial areas of the Islamabad and Rawalpindi. Plant root, shoot and the soil samples were collected and analyzed for selected metal concentration values. To evaluate the potential of plant species for phytoremediation: Bioconcentration Factor (BCF), Biological Accumulation Coefficient (BAC) and Biological Transfer Coefficient (BCF) were calculated. The concentration of Pb in soils varied from 2-29 mg/kg, Zn from 28.82-172.56 mg/ kg, Cu from 8.88-306 mg/kg, respectively. The concentration of Pb in plant shoots varied from 1.0 to 39 mg/kg, Zn from 17.25 to 194.03 mg/kg, Cu from 0.65 to 171.83 mg/kg. The concentration of Pb in roots of plant varied 1-43 mg/kg, Zn from 3.34-116.16 mg/kg, Cu from 3.35- 416.89 mg/kg. Brachiaria raptans and Malvastrum coromandelianum were found most suitable for phytostabilization of sites contaminated with Pb and Cu (BCF= 18 and 9.12). Considering the BAC values, 15 species for Pb, two species for Cu, five species for Zn possessed the characteristics of hyperaccumulator, none of the plant species was found as hyperaccumulator; however plants with high BCF (metal concentration ratio of plant root to soil) and low BTC (metal concentration ratio of plants shoots to roots) have the potential for phytostabilization and phytoextraction. The results of this study can be used for management and decontamination of soils with heavy metals using plant species having phytoremediation potential/characteristics.
    Pakistan Journal of Botany. 01/2011; 43:1925-1933.
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    ABSTRACT: In the present study a novel protocol for the removal of Albumin and Globulin in 10 genotypes of Pisum sativum were developed by using Osborn solubility class protein extraction buffer (10mM Tris-Hcl buffer, pH 6.8, 0.5 M NaCl). One major allocation of protein band and one minor protein band corresponding to 10 kda and 22 kda, respectively, was detected in SDS-PAGE (untreated cases). There is a need to further improve this method so that the minor groups of albumin and globulin could also be investigated further to characterize through 2D gel electrophoresis.
    Pakistan Journal of Botany. 01/2011; 43:157-164.
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    ABSTRACT: In the present study, we elucidated the effect of potassium salts on alkali denatured hen egg white lysozyme (EC 3.2.1.17) using intrinsic/extrinsic fluorescence as well circular dichroism (CD) spectroscopic methods. Intrinsic fluorescence studies revealed that various potassium salts mediate stabilization of lysozyme against alkali denaturation. Far and near UV CD spectrum studies, showed that 2M KCl induced appreciable amount of secondary structure with minimum tertiary contacts in lysozyme at pH 12.6. Acrylamide quenching studies suggest that at pH 12.6, the presence of 2M KCl causes reduced accessibility of the quencher to tryptophan residues of the protein presumably because of its compact conformation. In summary, the results of present study suggest that lysozyme attains a compact folded intermediate with molten globule like characteristics at alkaline pH in presence of potassium chloride.
    Protein and Peptide Letters 01/2010; 17(1):11-7. · 1.99 Impact Factor
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    ABSTRACT: Standardized ring-width chronologies of Pinus wallichiana A.B. Jackson and Pinus gerardiana Wall. ex Lamb., are presented, covering maximum period from AD 1300-2000 and 1400-2000AD respectively. These chronologies are based on highly correlated (0.636) and crossmatchable wood samples. Response function analysis was used to investigate the climatic signals in these chronologies. Both response function analysis were statistically significant at p<0.05 level. Kalash response function showed 40% while Astore explained 22% variance due to climate. It is shown that though both sampling area fall under dry temperate area, good growth in Pinus wallichiana (Astore) is associated with hot and dry conditions, while reverse is the case with Pinus gerardiana (Kalash). However, despite these differences, both species showed some similar effects, responses and trends. Therefore it may be concluded that both species are suitable for paleoclimatic reconstruction back to at least 500 years. It is also suggested that more and detailed investigations are required before making concrete conclusion and reconstruction of past climatic variations.
    Pakistan Journal of Botany. 01/2010; 42:731-738.
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    ABSTRACT: Forty one stands of conifer forests of moist temperate areas, covering the natural limits of this forest type, in northern Pakistan were investigated. Multivariate techniques including cluster analysis (Ward’s agglomerative method and TWINSPAN a divisive method) as well as ordination (DECORANA) were used to explore vegetation composition and structure of canopy trees and understorey (shrubs and herbs) vegetation and their relationship with the associated environmental factors. Classification of overstorey trees derived by TWINSPAN and Ward’s methods showed some similarities in groups. Among the topographic variables, only elevation was found to be significant (P < 0.01) while edaphic variables showed no significant difference in group means. For understorey vegetation some similarities were also recorded between TWINSPAN and Ward’s method. Among environmental variables elevation (P < 0.001), aspect (P< 0.05), canopy cover (P < 0.001) and soil pH (P < 0.01) were found to be significant. In many cases relationship of axes in DCA stand ordination and environmental variables were also significantly correlated, however axis two of understorey ordination did not show any significant correlation with any environmental variables. Present study showed similarities between Ward’s cluster analysis of tree vegetation and understorey vegetation data, despite a long history of anthropogenic disturbance in these areas.
    Pakistan Journal of Botany 01/2010; 42:267-293. · 1.21 Impact Factor
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    ABSTRACT: The present study is based on the examination of quantitative vegetation description of moist temperate conifer forests of Himalayan region of Pakistan. Forty one stands at five different locations from Himalayan Region of Pakistan were chosen for the study. The underlying group in the vegetation was exposed using Ward’s cluster analysis. The three groups derived from cluster analysis were superimposed on principal component analysis (PCA) ordination axes. Three main groups were clearly separated out on ordination plane (axis 1 and 2) Group I was dominated by Pinus wallichiana stands, group II was dominated by Abies pindrow while group III was composed of Cedrus deodara dominating stands. The relationships between environmental factors and vegetation were examined. Environmental variables generally showed no significant relation with ordination axes, among the environmental variables only elevation showed significant correlation with the ordination axes suggesting that the altitudinal gradient has an overriding role in the composition of vegetation. Moist temperate forests are highly disturbed due to anthropogenic causes. Some recommendations are proposed for the improvement and future research in these forests.
    International Journal of Biology and Biotechnology. 01/2010; 7:175-185..
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    ABSTRACT: An Ethno-veterinary study was conducted in the Marghazar Valley, district Swat Pakistan during summer 2006. A total of 49 Ethno veterinary important plant species were found to belong to 49 genera and 35 families. Among these families the largest family was Lamiaceae, having 5 species, the second largest family was Asteraceae having 4 species.
    International Journal of Biology and Biotechnology. 01/2010; 7:273-279.
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    ABSTRACT: Mangroves are woody plants that grow in tropical and sub tropical areas. The mangroves can exist under wide range of salinity, tidal amplitude, wind and temperature, even in muddy and anaerobic soil condition. An experiment was conducted in the University Campus to know the establishment, survival and growth of seedlings of Avicennia marina based on planting observations in water logged and saline silty loam of Karachi. Growth and physiological responses were recorded at seedlings stages. The area of seeds, height, number of leaves, area of leaves and number of pneumatophores were recorded. Physical and chemical analyses of soil and water were also carried out in order to get better information. The result showed that due to availability of oxygen, mangroves did not hold up complexity to respire that’s why could not exhibit pneumatophores. It is suggested that those areas which are fully saline and waterlogged and unable to support any other crop can be conserved by planting mangroves.
    Pakistan Journal of Botany. 01/2010; 42:2423-2428.
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    ABSTRACT: Floristic composition and communities of 47 stands of deodar (Cedrus deodara Roxb. ex Lamb. G. Don.) forests covering its natural limits in Pakistan are presented. On the basis of phytosociological analysis, floristic composition of tree species and importance value index, following deodar communities and a pure forest type are recognized and described quantitatively. Deodar – Juglans community, Deodar – Quercus community, Deodar – Taxus community, Deodar – Picea community, Deodar – Abies community, Deodar – Pinus gerardiana community, Deodar – Pinus wallichiana community and monospecific Deodar forests It is anticipated that poor floristic similarities on forest ground, between these communities, within the community and in pure stands are related to difference in climatic zones, microclimatic conditions and degree of disturbance. Can deodar compete with its associated species? Due to highly disturbed nature of these stands, no conclusion could be made.
    Pakistan Journal of Botany. 01/2010; 42:3091-3102.
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    ABSTRACT: A phytosociological study of Pinus roxburghii was undertaken in Lesser Himalayan and Hindu Kush range of Pakistan during summer 2005. Thirteen stands were sampled at five different locations i.e., Mansehra, Rawalpindi, Islamabad, Swat and Lower Dir ranging from 750 - 1700 meters elevation. P. roxburghii was forming pure vegetation in 12 stands but in one location, it was associated with some angiospermic tree species. Quantitative attributes viz. relative density, relative frequency and relative basal area and absolute values were calculated. Circular plots were used to assess the vegetation association on the forest floor. Pine seedlings were recorded in nine stands. The common angiospermic species were found in association with Chir pine like Dodonaea viscosa, Punica granatum, Erodium cicutarium, Medicago denticulate and Vicia sativa. The most rare species encountered were Ailanthus altissima, Daphne mucronata, Melia azedirach, Potentilla nepalensis, Urtica dioica and Olea ferruginea. It was also observed that the studied forests are unstable and degraded and would be vanished if not maintained properly.
    Pakistan Journal of Botany. 01/2009; 41:2357-2369.
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    ABSTRACT: Dendrochronological techniques were used in 49 different mature stands of 39 gymnosperms forests of Pakistan. Wood samples were obtained from 218 trees including Pinus wallichiana A.B. Jackson, Pinus roxburghii Sargent, Picea smithiana (Wall) Boiss., Cedrus deodara (Roxb.) G. Donf., Abies pindrow Royle and Taxus wallichiana Zucc., using Swedish Increment Borer to obtain age and growth rates of these trees. It showed that largest tree is not necessarily the oldest tree. Highest overall growth rate (2.65 ± 0.19 y/cm) was recorded in Cedrus deodara from south facing slopes while slowest growth was observed in Taxus wallichiana, from East facing slope. Except in Pinus roxburghii, Dbh and age showed no significant relation. In addition no significant relation was found between elevation and growth rates. Statistics of some selected species and sites are also presented and it is suggested that growth rings of these species could be used in dendrochronological research.
    Pakistan Journal of Botany. 01/2009; 41:849-860.