Cheng-Yu Lee

National Chiao Tung University, Hsin-chu-hsien, Taiwan, Taiwan

Are you Cheng-Yu Lee?

Claim your profile

Publications (4)16.57 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: We have developed a method, using high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS), to directly analyze metal ion impurities in liquid crystals (LCs). From measurements of resistivity, a commonly used technique for quality control of liquid crystal display (LCD) cells, we found that the resistivities of the LCs decreased significantly upon progressing through the different stages of the LCD cell manufacturing process. We determined the levels of metal ion impurities in the LCs through direct loading of diluted LC samples into the HR-ICP-MS system at a steady flow rate. Solutions featuring LC contents of 10% (v/v) were formed by dissolving each LC in isopropanol (IPA) containing acetone as a modifier. We quantified the trace metal ion impurities in the LC samples using a certified standard of metal ions diluted in IPA (external calibration) without the need for digestion pretreatment. Next, we compared the levels of metal ion impurities with the resistivities of the LCs at the different stages of the LCD cell manufacturing process. We confirmed that the resistivities of the LCs decreased as the total levels of metal ion impurities in the LCs increased. The total content of metal ion impurities added into the LC was higher in the siphoning process than in the one-drop filling process during the fabrication of LCD cells. The HR-ICP-MS method could, therefore, be used to directly measure metal ion impurities in LCs during the LCD cell manufacturing process, potentially replacing resistivity measurements of LCs as a means of quality control. We suggest setting 1400 ng L−1 as the limit of the total metal ion concentration in LCs used for LCD cell manufacturing. We used the HR-ICP-MS method to analyze a stained LCD panel to confirm that the content of metal ion impurities was indeed significantly greater in the stained area of the defected LCD. HR-ICP-MS appears to be a promising technique for the direct and effective analyses of metal ion impurities in LCs.
    Analytical methods 10/2012; 4(11):3631-3637. DOI:10.1039/C2AY25627D · 1.82 Impact Factor
  • Cheng-Yu Lee · Kuan-Ying Wu · Hsiu-Li Su · Huan-Yi Hung · You-Zung Hsieh ·
    [Show abstract] [Hide abstract]
    ABSTRACT: In this study, we developed an ultrasensitive label-free aptamer-based electrochemical biosensor, featuring a highly specific anti-human immunoglobulin E (IgE) aptamer as a capture probe, for human IgE detection. Construction of the aptasensor began with the electrodeposition of gold nanoparticles (AuNPs) onto a graphite-based screen-printed electrode (SPE). After immobilizing the thiol-capped anti-human IgE aptamer onto the AuNPs through self-assembly, we treated the electrode with mercaptohexanol (MCH) to ensure that the remaining unoccupied surfaces of the AuNPs would not undergo nonspecific binding. We employed a designed complementary DNA featuring a guanine-rich section in its sequence (cDNA G1) as a detection probe to bind with the unbound anti-human IgE aptamer. We measured the redox current of methylene blue (MB) to determine the concentration of human IgE in the sample. When the aptamer captured human IgE, the binding of cDNA G1 to the aptamer was inhibited. Using cDNA G1 in the assay greatly amplified the redox signal of MB bound to the detection probe. Accordingly, this approach allowed the linear range (coefficient of determination: 0.996) for the analysis of human IgE to extend from 1 to 100,000pM; the limit of detection was 0.16pM. The fabricated aptasensor exhibited good selectivity toward human IgE even when human IgG, thrombin, and human serum albumin were present at 100-fold concentrations. This method should be readily applicable to the detection of other analytes, merely by replacing the anti-human IgE aptamer/cDNA G1 pair with a suitable anti-target molecule aptamer and cDNA.
    Biosensors & Bioelectronics 07/2012; 39(1):133-8. DOI:10.1016/j.bios.2012.07.009 · 6.41 Impact Factor
  • Hsiu-Li Su · Wan-Chun Kao · Kuan-Wen Lin · Cheng-yu Lee · You-Zung Hsieh ·
    [Show abstract] [Hide abstract]
    ABSTRACT: In this study, we found that adding 1-butyl-3-methylimidazolium-based ionic liquids (ILs) and sodium dodecyl sulfate (SDS) as modifiers in the background electrolyte (BGE) for capillary electrophoresis enhanced the separation of benzodiazepines. In particular, 1-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide ([BMIM][NTf2]) was the best IL additive for the separation system because its anionic moiety interacted favorably with the benzodiazepines. We added SDS because of its known effect on the separation of hydrophobic analytes. We optimized the separation conditions in terms of the concentrations of the IL, SDS, and organic solvent, the pH, and the BGE's ionic strength. The optimal BGE, containing 170 mM [BMIM][NTf2] and 10 mM SDS, provided baseline separation, high efficiency, and satisfactory peak shapes for the benzodiazepines. The separation mechanism was based on heteroassociation between the anionic moiety of the IL and the benzodiazepines, with SDS improving the resolution of the separation. The limits of detection for the seven analytes ranged from 2.74 to 4.42 microg/mL. We subjected a urine sample to off-line solid phase extraction (SPE) prior to the analysis of its benzodiazepine content. Our experimental results reveal that the combination of [BMIM][NTf2] and SDS provides adequate separation efficiency for its application to CE analyses of benzodiazepines after SPE concentration.
    Journal of Chromatography A 03/2010; 1217(17):2973-9. DOI:10.1016/j.chroma.2010.02.056 · 4.17 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, nonaqueous capillary electrophoresis (NACE) was used to separate three open-cage fullerenes. Trifluoroacetic acid (TFA) was used as the nonaqueous background electrolyte to change the analytes' mobilities. The selectivity and separation efficiency were critically affected by the nature of the buffer system, the choice of organic solvent, and the concentrations of TFA and sodium acetate (NaOAc) in the background electrolyte. The optimized separation occurred using 200 mM TFA/20mM NaOAc in MeOH/acetonitrile (10:90, v/v), providing highly efficient baseline separation of the open-cage fullerenes within 5 min. The migration time repeatability for the three analytes was less than 1% (relative standard deviation). Thus, NACE is a rapid, useful alternative to high-performance liquid chromatography for the separation of open-cage fullerenes.
    Journal of Chromatography A 03/2010; 1217(26):4471-5. DOI:10.1016/j.chroma.2010.02.085 · 4.17 Impact Factor