Bolanle A Adeniyi

University of Ibadan, Ibadan, Oyo State, Nigeria

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Publications (18)23.22 Total impact

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    ABSTRACT: Helicobacter pylori is a Gram-negative bacillus that is associated with the development of gastritis and peptic ulcer disease (PUD). In Nigeria, leaf extracts of Eucalyptus torelliana F. Muell. are used in traditional medicine to treat PUD and other gastrointestinal ailments. The additive and synergistic effects of E. torelliana leaf extracts, in combination with clarithromycin, were investigated using two types of H. pylori strains (ATCC 43629, ATCC 43579) and four clinical isolates of H. pylori (Ed, A2, G1-1, 5514) in the checkerboard assay and the fractional inhibitory concentration (FIC) index. A time-kill study was also performed on the strain ATCC 43579. The results showed that the E. torelliana extract inhibited the growth of all H. pylori strains, and the addition of one of the isolated active compounds, namely compound 2 (a substituted pyrenyl ester) enhanced the activity of clarithromycin. The minimum inhibitory concentration values of clarithromycin and the botanical compound were reduced twofold (from 0.125 to 0.0625 µg/mL and > 100 to 50 µg/mL respectively). A 100% reduction in CFU/mL of H. pylori ATCC 43579 was observed with the combination of 0.25 µg/mL clarithromycin and 100 µg/mL and 200 µg/mL compound 2 after 3 h of exposure. The results of the investigation showed that the combination of botanical compounds and antibiotics may be beneficial in the treatment of H. pylori infections. Copyright © 2012 John Wiley & Sons, Ltd.
    Phytotherapy Research 02/2012; 26(9):1393-8. · 2.07 Impact Factor
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    African journal of microbiology research 01/2012; Vol. 6:3399-3402. · 0.54 Impact Factor
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    ABSTRACT: Helicobacter pylori consist in a helical shaped Gram-negative bacterium, approximately 3 micrometers long with a diameter of approximately 0.5 micrometers. It has 4-6 flagella. It is microaerophilic and tests positive for oxidase, catalase and urease. With its flagella, the bacterium moves through the stomach lumen and drills into the mucus gel layer of the stomach. In humans, H. pylori have been associated with peptic ulcers, chronic gastritis, duodenitis and stomach cancer. It is widely believed that in the absence of treatment, H. pylori infection, once established in its gastric niche, persists for life. The aim of this research is to study the cultural characteristics and antibiotic susceptibility pattern of H. pylori strains isolated from southwest Nigeria. The cultural characteristics and antibiotic susceptibility pattern of Helicobacter pylori strains isolated from gastric mucosal antral biopsy specimens collected from 43 of 52 dyspepsia patients in the University College Hospital Ibadan, Oyo State, Nigeria, were determined using standard microbiological methods for Helicobacter pylori isolation. The 43 isolates were subjected to 23 different antibiotics and each of the antibiotics demonstrated a variable degree of activity against the isolates. Among the antibiotics to which the organism was most susceptible are: ofloxacin (30 μg) 100% activity, ciprofloxacin (5 μg) 97.67% activity, gentamicin (120 μg) 95.35 activity, amikacin (30 μg), kanamycin (30 μg) and chloramphenicol (30 μg) each 90.70% activity, clarithromycin (15 μg) 93.02, while the less active antibiotics are: augmentin (30 μg) 23.26% active, amoxycillin (25 μg) and metronidazole (50 μg) each 27.91% active and clindamycin (2 mg) 30.23% active. From the result of the antibiotic susceptibility pattern of the strains of the organism, 95.35% of the total isolates are multi drug resistant. Resistance was developed to, among others, augmentin (30 μg), amoxycillin (25 μg), metronidazole (50 μg) and clindamycin (2 mg).
    Gastroenterology Insights. 01/2012; Vol. 4(e 21):87-89.
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    ABSTRACT: Eucalyptus camaldulensis Dehnh. (Myrtaceae) and Eucalyptus torelliana F. Muell are used in Nigerian traditional medicine for the treatment of cough associated with tuberculosis (TB) and other respiratory infections. Hexane, chloroform, methanol extracts, and isolated compounds of E. camaldulensis and E. torelliana were screened for activity against Mycobacterium tuberculosis H37Rv (MtbH37Rv) to authenticate the traditional use of these plants. The microplate alamar blue assay (MABA) method was used to investigate the anti-M. tuberculosis activities. Bioassay-guided fractionation of the hexane extract of E. torelliana leaf was performed, and isolated compounds were characterized by MS, 1D- and 2D-NMR. The extracts inhibited the growth of MtbH37Rv [minimum inhibitory concentration (MIC) 4-64 µg/mL]. Spectroscopic characterization led to the identification of two compounds, hydroxymyristic acid methylester (1) and a substituted pyrenyl ester, a sterol (2). Compounds 1 and 2 had MIC of 49.45 and 46.99 µg/mL; IC(50) >100 and 38.21 µg/mL; selectivity index (SI) >2.02 and 0.81, respectively, and a minimum bactericidal concentration (MBC) of 62.50 µg/mL. The anti-TB activities of these plants on M. tuberculosis H37Rv support their use in traditional medicine for the treatment of coughs associated with TB and reveals the presence of anti-Mtb active compounds in the plants. These findings not only demonstrate a new potential area of therapeutic value of E. camaldulensis and E. torelliana, but also illustrate the role of esters as anti-Mtb active principles in ethnobotanical preparations and as lead compounds in the development of new and effective anti-Mtb drugs.
    Pharmaceutical Biology 12/2011; 50(1):92-8. · 1.21 Impact Factor
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    ABSTRACT: The extracts of Eucalyptus camaldulensis Dehnh. and Eucalyptus torelliana F. Muell. (Myrtaceae) were screened against four non-tuberculous mycobacteria species: Mycobacterium fortuitum ATCC 684, Mycobacterium smegmatis ATCC 19420, Mycobacterium phlei ATCC 19240 and Mycobacterium abscessus. The agar diffusion method was used to investigate the activity of these plants at 1 and 2 mg/ml concentration. The methanol extracts exhibited the highest activity against the test organisms, the most susceptible being M. fortuitum ATCC 684. The minimum inhibitory concentration (MIC) value for the bioactive extracts ranged between 1 to 2 mg/ml while minimum bactericidal concentration (MBC) value was 2 to 4 mg/ml. Bactericidal activity of the methanol extracts of E. camaldulensis on M. fortuitum ATCC 684 was investigated using the viable counting technique. The leaf extracts at 4 mg/ml (4 × MIC) and 8 mg/ml (8 × MIC) gave 80.49 and 100% kill of the organism respectively at 24 h exposure time while the stem bark extracts at the same concentrations afforded 67.35 and 100% kill respectively after 24 h of exposure. The activities demonstrated by these plants support their use in the treatment of cough associated with most pulmonary diseases; and suggest that these plants may be of therapeutic importance for the treatment of infections caused by the non-tuberculous mycobacteria (NTM).
    African journal of microbiology research 11/2011; 5:3652-3657. · 0.54 Impact Factor
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    ABSTRACT: The characterisation of 24 lactic acid bacteria (LAB) isolates from Nigerian traditional fermented dairy foods, including some cow's intestine isolates, was conducted in order to select isolates for potential use as probiotics. LAB isolates were identified by partial sequencing the 16S rRNA gene as belonging to the species Lactobacillus paracasei, Lactobacillus brevis and mainly Weissella confusa. At the end of a characterisation process, 2 L. paracasei and 2 W. confusa isolates were selected, and their resistance to a simulated gastrointestinal digestion and their ability to adhere to eukaryotic cell lines were assessed. The survival to the simulated gastrointestinal passage was higher when bacterial suspensions were made in skimmed milk (2.0±0.8 log units reduction) or at the simulated gastric juice pH 3 (2.7±0.9 log units reduction) than at pH 2.0 (5.5±0.7 log units reduction). Adhesion of LAB to both intestinal and vaginal epithelial models was comparable or higher than that of the reference Lactobacillus rhamnosus GG. However, some of the isolates increased the adhesion of the pathogen Escherichia coli LMG2092 to HT-29 and HeLa monolayers. Overall, isolates L. paracasei UI14 and W. confusa UI7 are good candidates for further studying potential benefits that support their use as probiotics. This is one of the few articles reporting the characterisation and the probiotic potential of Weissella, although more studies are needed in order to establish their safety for potential probiotic applications.
    International journal of food microbiology 05/2011; 147(2):97-104. · 3.01 Impact Factor
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    ABSTRACT: Background and Objective: Diagnostic tests currently in use for the detection of Helicobacter pylori have been classified into either non-invasive or invasive categories, with each having its merits and demerits, as well as superiority over the other depending on the clinical setting. This study compared the accuracy of the urea breath test, histology, serology and stool antigen assay using culture as the gold standard in the diagnosis in our clinical setting. Method: Blood, stool, fibre-optic endoscopic gastric biopsies and breath were tested in 52 consenting dyspeptic patients for the detection of H. pylori infection. Results: The Negative Predictive Values, Positive Predictive Values and the diagnostic accuracy obtained were 40%, 73.7%, 47.1%; 8.3% 80.6%, 62.5%; 12.5%, 94.1%, 68%; and 30%, 93.8%, 69% for Faecal antigen test, Serology, Histology and Urea breath test respectively. Conclusion: Our findings show that histology is reliable for definitive diagnosis of H. pylori infection in dyspeptic patients in our setting and where facilities are available; UBT can be used as a non-invasive method of assessment of eradication of infection.
    Nigerian Journal of Gastrienterology and Hepatology. 01/2011; Vol. 3:31-38.
  • 08/2009: pages 127 - 135; , ISBN: 9783527622931
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    ABSTRACT: A total of 96 lactic acid bacteria (LAB) were isolated from African indigenous fermented products and cow's intestines to study their inhibitory capability against multi-drug-resistant uropathogens. Escherichia coli accounted for approximately 45% of isolated uropathogens, followed by Staphylococcus spp. (20%). The Gram negative uropathogens were highly resistant to quinolones, co-trimoxazole, teicoplanin and some beta-lactams, while the Staphylococcus spp. showed high resistance to aminoglycosides, beta-lactams and macrolides. Twenty-four LAB isolates were selected based on their antimicrobial activity against two uropathogenic Staphylococcus aureus strains and bacteriocin production. LAB strains showing antimicrobial activity were grouped into smaller groups through amplified ribosomal DNA restriction analysis (ARDRA). Representative strains were identified as Weissella spp., Enterococcus faecium, Lactococcus lactis and Lactobacillus brevis through sequencing of 16S rDNA. The Weissella spp. and L. brevis strains demonstrated remarkable inhibitory activity against seven strains of Gram negative uropathogens. Two strains of L. lactis produced a bacteriocin-like inhibitory substance active against Lactobacillus sakei. In this study, an unusual high rate of co-trimoxazole, quinolones and macrolides resistance among uropathogens from south west Nigeria was discovered. Based on their sensitivity to Weissella spp., there is a potential for using these LAB as a natural approach for the protection against the uropathogens assayed.
    Archives of Microbiology 07/2009; 191(8):639-48. · 1.91 Impact Factor
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    ABSTRACT: Previous investigations demonstrated that a standardized extract of ginger rhizome inhibited the growth of Helicobacter pylori in vitro with a minimum inhibitory concentration in the range 0.78 to 12.5 mug/mL. In the present work, the extract was tested in a rodent model of H. pylori-induced disease, the Mongolian gerbil, to examine the effects of the extract on both prevention and eradication of infection. The extract was administered to Mongolian gerbils at a daily dose of 100 mg/kg body weight in rations either 3 weeks prior to infection or 6 weeks post-infection. Treatment with the standardized ginger extract reduced H. pylori load as compared with controls and significantly (P<0.05) reduced both acute and chronic muscosal and submucosal inflammation, cryptitis, as well as epithelial cell degeneration and erosion induced by H. pylori. Importantly, the extract did not increase morbidity or mortality. Further investigations of the mechanism demonstrated that the ginger extract inhibited the activity of cyclooxygenase-2, with 50% inhibitory concentration (IC(50)) of 8.5 mug/mL in vitro, inhibited the nuclear factor-kappaB transcriptional response in kBZ Jurkat cells (human T lymphocytes) with an IC(50) of 24.6 mug/mL, and significantly inhibited the release of interleukin (IL)-1beta, IL-6, IL-8, and tumor necrosis factor-alpha from lipopolysaccharide-stimulated human peripheral blood mononuclear cells with IC(50) values of 3.89, 7.7, 8.5, and 8.37 mug/mL, respectively. These results suggest ginger extracts may be useful for development as agents to reduce H. pylori-induced inflammation and as for gastric cancer chemoprevention.
    Pharmaceutical Biology 01/2009; 47(1):92-98. · 1.21 Impact Factor
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    ABSTRACT: Helicobacter pylori is the primary etiologic agent of peptic ulcer, duodenal ulcer, chronic gastritis, gastric adenocarcinoma and related gastroduodenal disorders. Current triple therapy, including antibiotics and proton-pump inhibitors, has been successful; however, adverse events, non-patient compliance and consequent relapse of Helicobacter pylori infections are common. Crude methanol extracts of Eucalyptus grandis Hill ex. Maiden (Myrtaceae) stem bark were screened against a standard strain ATCC 43504 and ten clinical strains of H. pylori using the agar diffusion method on Mueller-Hinton agar supplemented with defibrinated horse blood and grown in a microaerophilic incubator. All the strains except UCH 97002 and UCH 98020 were inhibited by the extract to varying degrees. The minimum inhibitory concentration (MIC) against the susceptible strains tested ranged from 0.39 and 1.56 μg/mL. The urease activity of the three H. pylori strains tested decreased with increasing concentrations of the extract. The greatest inhibition of urease activity was observed in clinical strain UCH 97009. In addition, methanol extracts of the E. grandis enhanced cell aggregation of seven of the H. pylori strains leading to a decrease in the cell surface hydrophobicity. The salt aggregation test titer decreased from >3 to <1.5 for five of the strains and to <3 for two of the strains. Phytochemical screening of the plant revealed the presence of tannins, essential oils and saponins, while alkaloids were not detected. The anti-Helicobacter pylori activity observed in this study correlates well with the traditional use of this plant in Nigeria.
    12/2008; 47(1):13-17.
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    Olusegun O Soge, Bolanle A Adeniyi, Marilyn C Roberts
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    ABSTRACT: To determine antibiotic resistance genes associated with 17 Nigerian CTX-M-positive Klebsiella pneumoniae plasmids from patients with community-acquired urinary tract infections. The size and restriction patterns of the plasmids were determined, and antibiotic resistance genes were identified using DNA-DNA hybridization, PCR assays, hybridization of PCR products with internal probes, and sequencing. All CTX-M plasmids were large (58-320 kb) and carried the following genes: aac(6')-Ib (aminoglycoside resistance) which included aac(6')-Ib-cr (aminoglycoside-fluoroquinolone resistance), aadA2 (aminoglycoside resistance), erm(B) (macrolide-lincosamide-streptogramin B resistance), blaTEM-1 (ampicillin resistance), tet(A) (tetracycline resistance), sul1 (sulphonamide resistance), dfr (trimethoprim resistance) and intI1, an integrase associated with class 1 integrons. Eleven (65%) plasmids carried an mph(A) gene (macrolide resistance), seven (41%) plasmids carried a qnrB1 gene (low-level quinolone resistance) and four (24%) plasmids carried multiple cat genes (chloramphenicol resistance). catA2, catA3 and qnrB1 genes and a 6 kb PstI fragment, carrying the blaCTX-M gene, were sequenced. This is the first description of catA2 and catA3 genes in Klebsiella spp. and the first description of the erm(B) and floR genes associated with a CTX-M plasmid. This is also the first report of qnrB1 and aac(6')-Ib-cr in isolates from Africa and the first report of these two genes on the same plasmid.
    Journal of Antimicrobial Chemotherapy 12/2006; 58(5):1048-53. · 5.34 Impact Factor
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    ABSTRACT: In this study, extended-spectrum beta-lactamases (ESBLs) were characterized from 30 selected multidrug-resistant Klebsiella pneumoniae strains isolated from patients with community-acquired urinary tract infections from Southwest Nigeria. The beta-lactamases were phenotypically characterized using isoelectric focusing, genotypically characterized using PCR assays and hybridization of the PCR products. Two of the bla(CTX-M) genes were completely sequenced. The location of the CTX-M-type genes was determined using transformation, DNA-DNA hybridization, PCR assays and hybridization of the PCR products from the Escherichia coli transformants. All 30 isolates produced at least one beta-lactamase. Seventeen of the isolates were resistant to cefotaxime, and had > or =100-fold reduction in susceptibility with cefotaxime plus clavulanic acid (4 mg/L), indicating the presence of an ESBL. The 17 isolates were shown to have bla(CTX-M) genes that were associated with large plasmids (> or =58 kb), which also carried a tetracycline resistance gene, tet(A), and various aminoglycoside resistance genes. Two CTX-M-type genes were sequenced and had amino acid sequences indistinguishable from previously sequenced CTX-M-15 beta-lactamases. The ISEcp1 element was located upstream of bla(CTX-M-15) in the same position as previously described. In addition, 23 of the isolates produced TEM beta-lactamases, 27 produced SHV beta-lactamases and four produced AmpC beta-lactamases. Thirty K. pneumoniae produced multiple beta-lactamases, with 57% producing CTX-M enzymes. This is the first characterization of CTX-M-15-positive K. pneumoniae in Western Africa.
    Journal of Antimicrobial Chemotherapy 01/2006; 57(1):24-30. · 5.34 Impact Factor
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    Adeniyi B.A, Lawal T.O, Olaleye S.B
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    ABSTRACT: The crude extracts of Eucalyptus camaldulensis were investigated for their in vitro antimicrobial and gastro-protective activities in albino rats. The antimicrobial activity was investigated by screening the crude plant extract for activity against Candida albicans and clinically isolated gentamycin resistant wound bacteria viz: Staphylococcus aureus UCH 2600, Escherichia coli UCH 2554, Pseudomonas aeruginosa UCH 2780 and Proteus mirabilis UCH 2773. These microorganisms were susceptible to the crude extracts at a 10 mg mL<sup>-1</sup> concentration. The gastroprotective activity of the methanol extract of Eucalyptus camaldulensis (MEEC) was investigated in rats. Gastro protection was evaluated against gastric mucosal damage induced by ethanol/HCl mixture. The Hcl/Ethanol mixture (1.5 mL of 0.15 N HCl in 70% ethanol) caused severe gastric damage with ulcer index 2.7�0.33. Pre-treatment of animals with crude extract of Eucalyptus camaldulensis leaf 200 and 1000 mg kg<sup>-1</sup> orally for 1 h significantly reduced the formation of ulcer by the HCl/ethanol mixture with preventive ratios of 56 and 89%, respectively. Ranitidine afforded 92.5% protection. The results suggest that crude extracts of Eucalyptus camaldulensis has both antimicrobial and anti-ulcer properties.
    Journal of Biological Sciences. 01/2006;
  • Adeniyi B.A, Ayeni F.A, Ogunbanwo S.T
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    ABSTRACT: Five Lactic Acid Bacteria (LAB) were isolated from different indigenous fermented dairy foods and identified as Lactobacillus fermentum , L. brevis , L . plantarum , Lactococcus lactis and Streptococcus durans . The cell free supernatant of the selected LAB were able to inhibit the growth of all organisms implicated in urinary tract infection (UTI) used in this study. The largest zone of inhibition was produced by Lac. lactis K3 against Staphylococcus saprophyticus UCH 2051. The sensitivity of UTI pathogens to different antibiotics was investigated. The entire Gram-negative pathogens from UTI showed 100% resistance to Colistin, Augmentin, Nalidix acid, Nitrofuranton and Cotrimoxazole while most Gram positive pathogens from UTI were sensitive to different antibiotics used against them. The tested LAB produced various antimicrobial compounds such as organic acid, hydrogen peroxide and diacetyl. The highest yield of lactic acid (1.87 g L<sup>-1</sup>) was produced by Streptococcus durans K4 while the highest yield of diacetyl (2.324 g L<sup>-1</sup>) was produced by L. brevis M5 and the highest yield of hydrogen peroxide (0.00544 g L<sup>-1</sup>) was produced by L . plantarum N<sub>2</sub>.
    Biotechnology. 01/2006;
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    Adeniyi B.A, Odufowoke R.O, Olaleye S.B
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    ABSTRACT: The antibacterial and gastroprotective properties of crude extracts of Eucalyptus torelliana were investigated. Antibacterial activity was investigated by screening the crude extracts for activity against clinically isolated strains of wound bacteria viz., Staphylococcus aureus UCH 2010, Pseudomonas aeruginosa UCH 2125, Escherichia coli UCH 2007, Klebsiella species UCH 2694 and Proteus mirabilis CHO 2014. The dichloromethane crude extracts demonstrated highest antibacterial activity against all tested microorganisms at 10 mg mL<sup>-1</sup> concentration. The gastroprotective effect of the crude extract of the leaf was investigated in albino rats. This was evaluated against gastric mucosal damage induced by ethanol/HCl mixture. Ethanol/HCl mixture (1.5 mL of 0.15 N HCl in 70% ethanol) caused severe gastric mucosal damage with ulcer index of 2.7±0.33. Pre-treatment of animals with crude extract of Eucalyptus torelliana leaf 200 and 1000 mg kg<sup>-1</sup> orally for 1h significantly reduced the Pre-treatment of animals with 50 mg kg <sup>-1</sup>. Ranitidine for 1 h reduced the reduced the formation of ulcer by the ethanol HCl mixture with preventive ratios 56 and 92.5%, respectively. Ranitidine (50 mg kg<sup>-1</sup>) afforded 92.5% protection. The results therefore suggest that crude extracts of Eucalyptus torelliana possess both antibacterial and gastroprotective properties.
    International Journal of Pharmacology. 01/2006;
  • Bolanle A Adeniyi, Festus M Anyiam
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    ABSTRACT: The crude methanol extract of the leaf of Allium ascalonicum was screened in vitro against fi ve strains of Helicobacter pylori (Hp) (ATCC 24376, UCH 97001, UCH 97009, UCH 98026 and UCH 99039) for antibacterial activity by the agar diffusion method in Mueller-Hinton agar supplemented with de fi brinated horse blood. All the strains were inhibited by the extract to varying degrees. The minimum inhibitory concentrations (MICs) of the extract against all the tested strains ranged from 6.25 to 12.5 mg/mL. The effects of increasing concentrations of the extract on the urease activity of three of the Helicobacter pylori strains were investigated further. The results showed that increasing the concentration of the extract decreased the urease activity of all the strains tested. Phytochemical screening of the plant showed that it contains alkaloids, cardiac glycosides and saponins. The anti-Hp activity observed is discussed in relation to the chemical constituents reportedly isolated from these plants and their traditional uses. The result of this work suggests that Allium ascalonicum has some therapeutic potential against Helicobacter pylori infection, which could be explored for patients with gastroduodenal disorders.
    Phytotherapy Research 06/2004; 18(5):358-61. · 2.07 Impact Factor
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    ABSTRACT: In Thailand, traditional plant-based medicines have always been used to treat gastrointestinal ailments, including gastritis, peptic ulcer disease (PUD) and diarrhea. Since Helicobacter pylori (HP) is an etiological agent of PUD, we have used an ethnomedical approach for screening plant extracts as potential treatments for HP infections, including over 20 species from Thailand. International Memoranda of Agreement were established between UIC and Mahidol University in Thailand. Medicinal plants were collected, identified and extracted. Susceptibility testing was performed with 15 HP strains using the agar dilution procedure guidelines of the Clinical and Laboratory Standards Institute. In vivo studies included evaluating bacterial load, as well as acute and chronic inflammation in HP-infected Mongolian gerbils. Extracts of Curcuma longa L. and Boesenbergia rotunda (L.) Mansf. significantly reduced HP-induced gastric lesions, as assessed both macroscopically and microscopically in Mongolian gerbils. The treatments reduced acute and/or chronic inflammation in a prevention model of HP-induced gastritis.

Publication Stats

101 Citations
23.22 Total Impact Points

Institutions

  • 2004–2012
    • University of Ibadan
      • Department of Pharmaceutical Microbiology
      Ibadan, Oyo State, Nigeria
  • 2009
    • Vanderbilt University
      Nashville, Michigan, United States
    • University of Illinois at Chicago
      • Department of Pharmacy Practice
      Chicago, IL, United States