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ABSTRACT: Viruses can reorganize the cytoskeleton and restructure the host cell transport machinery. During infection viruses use different
cellular cues and signals to enlist the cytoskeleton for their mission. However, each virus specifically affects the cytoskeleton
structure. Thus, the aim of our study was to investigate the cytoskeletal changes in homologous equine dermal (ED) and heterologous
Vero cell lines infected with either equine herpesvirus 1 (EHV-1) strain Rac-H or Jan-E. We found that Rac-H strain disrupted
actin fibers and reduced F-actin level in ED cells, whereas the virus did not influence Vero cell cytoskeleton. Conversely,
the Jan-E strain induced polymerization of both F-actin and MT in Vero cells, but not in ED cells. Confocal-microscopy analysis
revealed that α-tubulin colocalized with viral antigen in ED cells infected with either Rac-H or Jan-E viruses. Alterations
in F-actin and α-tubulin were evaluated by confocal microscopy, Microimage analysis and scanning cytometry. This unique combination
allowed precise interpretation of confocal-based images showing the cellular events induced by EHV-1. We conclude that examination
of viral-induced pathogenic effects in species specific cell lines is more symptomatic than in heterologous cell lines.
Archives of Virology 04/2012; 155(5):733-743. · 2.11 Impact Factor
