Purim Jarujamrus

Publications (3)12.53 Total impact

• Article: A strategy to enhance the wettability of bioacive paper-based sensors.

  Junfei Tian, Purim Jarujamrus, Lizi Li, Miaosi Li, Wei Shen
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  ABSTRACT: This paper reports a potential method that can restore the wettability of bioactive paper-based sensors while maintaining their bioactivity. This study is driven by the need to increase the wettability of the antibody-loaded blood typing paper devices in order to increase the blood typing assaying speed using such paper devices. Plasma treatment is used to improve the wettability of bioactive paper; the protective effect of bovine serum albumin (BSA) to biomolecules against plasma deactivation is investigated. In the first stage, horseraddish peroxidase (HRP) was used as a model biomolecule, because of the convenience of its quantifiable colorimetric reaction with a substrate. By using this protection approach, the inactivation of biomolecules on paper during the plasma treatment is significantly slowed down. This approach enables plasma treatment to be used for fabricating paper-based bioactive sensors to achieve strong wettability for rapid penetration of liquid samples or reagents. Finally, we demonstrate the use of plasma treatment to increase the wettability of antibody treated blood typing paper. After the treatment, the blood typing paper becomes highly wettable; it allows much faster penetration of blood samples into the plasma treated testing paper. Antibodies on the paper are still sufficiently active for blood typing and can report patients' blood type accurately.
  ACS Applied Materials & Interfaces 11/2012; · 4.53 Impact Factor
• Article: Mechanisms of red blood cells agglutination in antibody-treated paper.

  Purim Jarujamrus, Junfei Tian, Xu Li, Atitaya Siripinyanond, Juwadee Shiowatana, Wei Shen
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  ABSTRACT: Recent reports on using bio-active paper and bio-active thread to determine human blood type have shown a tremendous potential of using these low-cost materials to build bio-sensors for blood diagnosis. In this work we focus on understanding the mechanisms of red blood cell agglutination in the antibody-loaded paper. We semi-quantitatively evaluate the percentage of antibody molecules that are adsorbed on cellulose fibres and can potentially immobilize red blood cells on the fibre surface, and the percentage of the molecules that can desorb from the cellulose fibre surface into the blood sample and cause haemagglutination reaction in the bulk of a blood sample. Our results show that 34 to 42% of antibody molecules in the papers treated with commercial blood grouping antibodies can desorb from the fibre surface. When specific antibody molecules are released into the blood sample via desorption, haemagglutination reaction occurs in the blood sample. The reaction bridges the red cells in the blood sample bulk to the layer of red cells immobilized on the fibre surface by the adsorbed antibody molecules. The desorbed antibody also causes agglutinated lumps of red blood cells to form. These lumps cannot pass through the pores of the filter paper. The immobilization and filtration of agglutinated red cells give reproducible identification of positive haemagglutination reaction. Results from this study provide information for designing new bio-active paper-based devices for human blood typing with improved sensitivity and specificity.
  The Analyst 03/2012; 137(9):2205-10. · 4.23 Impact Factor
• Article: Flow field-flow fractionation: a versatile approach for size characterization of alpha-tocopherol-induced enlargement of gold nanoparticles.

  Wimut Sermsri, Purim Jarujamrus, Juwadee Shiowatana, Atitaya Siripinyanond
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  ABSTRACT: Flow field-flow fractionation (FlFFF) was used for size characterization of gold nanoparticles. The measured particle sizes obtained from FlFFF for the commercial 10 nm gold nanoparticle standard and the gold nanoparticles synthesized in the laboratory were in good agreement with those measured by transmission electron microscopy (TEM). Further, the capability of alpha-tocopherol to induce enlargement of gold nanoparticles by catalysis of the reduction of AuCl(4)(-) by citrate was observed by monitoring the changes in particle size of gold nanoparticles using FlFFF. The effects of alpha-tocopherol and incubation time on enlargement of the gold nanoparticles were examined. Higher concentrations of alpha-tocopherol resulted in larger nanoparticles. At fixed alpha-tocopherol concentration, larger nanoparticles were formed at longer incubation times.
  Analytical and Bioanalytical Chemistry 02/2010; 396(8):3079-85. · 3.78 Impact Factor