Joan Casado

VHIR Vall d’Hebron Research Institute, Barcino, Catalonia, Spain

Are you Joan Casado?

Claim your profile

Publications (4)16.46 Total impact

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: BACKGROUND: Diabetic macular edema (DME) is the main cause of visual impairment in diabetic patients. The aim of the present study was to explore the differential proteomic pattern of the vitreous fluid from DME patients by means of fluorescence-based difference gel electrophoresis (DIGE). METHODS: Samples of vitreous from 8 type 2 diabetic patients (4 with DME without proliferative diabetic retinopathy [PDR] and 4 with PDR without DME), and 8 from non-diabetic subjects with idiopathic macular hole (control group) were selected from our vitreous bank for proteomic analysis. To further confirm the potential candidates identified by DIGE eighteen additional samples (6 PDR, 6 DME and 6 MH, matched by age) were analyzed by ELISA. RESULTS: Selecting an abundance ratio of 1.5-fold, p < 0.05, as the threshold for the study, 4 proteins were specifically associated with DME. Hemopexin was significantly higher in the vitreous fluid of patients with DME in comparison with both control subjects and PDR patients. By contrast, clusterin, transthyretin and crystallin S were significantly decreased in the vitreous of patients with DME. The differential production of hemopexin, clusterin and transthyretin was further confirmed by ELISA. CONCLUSIONS: Proteomic analysis by DIGE was useful in identifying new potential candidates involved in the pathogenesis of DME. These results could open up new strategies in the treatment of DME. This article is protected by copyright. All rights reserved.
    Diabetes/Metabolism Research and Reviews 04/2013; · 2.97 Impact Factor
  • Source
    Diabetes care 07/2010; 33(7):e92. · 7.74 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The purpose of this study was to compare intravitreous levels of lipopolysaccharide-binding protein and soluble CD14 (sCD14) between patients with proliferative diabetic retinopathy (PDR) and nondiabetic subjects. This study included 19 consecutive Type 2 diabetic patients with PDR in whom a vitrectomy was performed. Sixteen vitreous humors from nondiabetic patients matched by age, with idiopathic macular holes, were selected from our vitreous bank and used as a control group. Lipopolysaccharide-binding protein was assessed by enzyme-linked immunosorbent assay and sCD14 by a solid-phase enzyme-amplified sensitive immunoassay. Lipopolysaccharide-binding protein and sCD14 levels were significantly higher in patients with PDR than in the control group (lipopolysaccharide-binding protein, P < 0.001; sCD14, P < 0.01). After correcting for vitreal proteins, the results remained significantly higher in patients with PDR. No differences in serum levels were observed, and we did not find any correlation between serum and vitreous levels. A direct correlation between lipopolysaccharide-binding protein and sCD14 was detected in the vitreous fluid (r = 0.57; P < 0.001) but not in the plasma. Finally, a significant correlation between intravitreal levels of both lipopolysaccharide-binding protein and sCD14 and interleukin-8 and monocyte chemotactic protein-1 was also detected. Lipopolysaccharide-binding protein and sCD14 are elevated in the vitreous fluid of patients with PDR and thus may play a role in the innate immune response triggered by the inflammatory injury characteristic of PDR.
    Retina (Philadelphia, Pa.) 02/2010; 30(2):345-52. · 2.93 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: The purpose of this study was to compare intravitreous levels of lipopolysaccharide-binding protein and soluble CD14 (sCD14) between patients with proliferative diabetic retinopathy (PDR) and nondiabetic subjects. Methods: This study included 19 consecutive Type 2 diabetic patients with PDR in whom a vitrectomy was performed. Sixteen vitreous humors from nondiabetic patients matched by age, with idiopathic macular holes, were selected from our vitreous bank and used as a control group. Lipopolysaccharide-binding protein was assessed by enzyme-linked immunosorbent assay and sCD14 by a solid-phase enzyme-amplified sensitive immunoassay. Results: Lipopolysaccharide-binding protein and sCD14 levels were significantly higher in patients with PDR than in the control group (lipopolysaccharide-binding protein, P < 0.001; sCD14, P < 0.01). After correcting for vitreal proteins, the results remained significantly higher in patients with PDR. No differences in serum levels were observed, and we did not find any correlation between serum and vitreous levels. A direct correlation between lipopolysaccharide-binding protein and sCD14 was detected in the vitreous fluid (r = 0.57; P < 0.001) but not in the plasma. Finally, a significant correlation between intravitreal levels of both lipopolysaccharide-binding protein and sCD14 and interleukin-8 and monocyte chemotactic protein-1 was also detected. Conclusion: Lipopolysaccharide-binding protein and sCD14 are elevated in the vitreous fluid of patients with PDR and thus may play a role in the innate immune response triggered by the inflammatory injury characteristic of PDR.
    Retina 01/2010; 30(2):345-352. · 2.83 Impact Factor

Publication Stats

6 Citations
16.46 Total Impact Points

Institutions

  • 2013
    • VHIR Vall d’Hebron Research Institute
      Barcino, Catalonia, Spain
    • Hospital Universitari Mutua de Terrassa
      Terrassa, Catalonia, Spain
  • 2010
    • Autonomous University of Barcelona
      Cerdanyola del Vallès, Catalonia, Spain