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ABSTRACT: Major histocompatibility (MHC) class II molecules are cell surface glycoproteins that present extracellular antigens to CD4(+) T cells and are essential for initiation of the adaptive immune response. MHC class II expression requires recruitment of a master regulator, the class II transactivator (CIITA), to the MHC class II promoter. Post-translational modifications to CIITA play important roles in modulating CIITA mediated transcription of various genes in different cell types. We have previously linked regulation of CIITA to the Ubiquitin Proteasome System (UPS), and we and others have demonstrated that mono-ubiquitination of CIITA dramatically increases its transactivity whereas poly-ubiquitination leads to CIITA degradation. Here we identify three degron proximal lysine residues, Lys-315, Lys-330, and Lys-333, and a phosphorylation site, Ser-280, located within the CIITA degron, that regulate CIITA ubiquitination, stability, and MHC class II expression. Together, these findings contribute to the developing post-translational modification code for CIITA.
Journal of Biological Chemistry 08/2010; 285(34):25893-903. · 4.77 Impact Factor
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[show abstract]
[hide abstract]
ABSTRACT: Major histocompatibility (MHC) class II molecules are cell surface glycoproteins that present extracellular antigens to CD4+
T cells and are essential for initiation of the adaptive immune response. MHC class II expression requires recruitment of
a master regulator, the class II transactivator (CIITA), to the MHC class II promoter. Post-translational modifications to
CIITA play important roles in modulating CIITA mediated transcription of various genes in different cell types. We have previously
linked regulation of CIITA to the Ubiquitin Proteasome System (UPS) and we and others have demonstrated that mono-ubiquitination
of CIITA dramatically increases its transactivity whereas poly-ubiquitination leads to CIITA degradation. Here we identify
three degron proximal lysine residues, K315, K330, and K333, and a phosphorylation site, S280, located within the CIITA degron,
that regulate CIITA ubiquitination, stability, and MHC class II expression. Together, these findings contribute to the developing
post-translational modification code for CIITA.
Journal of Biological Chemistry 06/2010; · 4.77 Impact Factor
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[show abstract]
[hide abstract]
ABSTRACT: Major histocompatibility class II (MHC class II) molecules are glycoproteins that present extracellular antigens to CD4(+) T cells and are essential for initiation of adaptive immune responses. MHC class II expression requires recruitment of a master regulator, the class II transactivator (CIITA), to the MHC class II promoter. Others and we have earlier linked CIITA to the ubiquitin-proteasome system by showing that mono-ubiquitination of CIITA increases its transactivity, whereas poly-ubiquitination of CIITA leads to its degradation. We have further shown that the 26S proteasome also has non-proteolytic functions in MHC class II transcription, as 19S ATPase subunits of the 26S proteasome positively regulate MHC class II transcription and are necessary for stable promoter binding of CIITA. Although these basic requirements of the proteasome to initiate MHC class II transcription are known, how CIITA is recruited, stabilized, and degraded remains unclear. Here, we identify a novel N-terminal 19S ATPase-binding domain of CIITA. The ATPase-binding domain lies within the proline/serine/threonine-rich region of CIITA and encompasses a majority of the CIITA degron sequence. Absence of the ATPase-binding domain increases the half-life of CIITA, but blocks MHC class II surface expression, indicating that CIITA requires interaction with the 19S ATPases for both appropriate deployment and destruction.
Immunology and Cell Biology 03/2010; 88(8):807-16. · 3.66 Impact Factor
