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Publications (4)4.76 Total impact

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    ABSTRACT: In patients with immunological disorders, adenovirus infections are associated with significant rates of morbidity and mortality. Only few hematological units use molecular virological methods, such as polymerase chain reaction, for surveillance of adenovirus infection, and treatment strategies have never been evaluated in multicenter clinical trials. This report describes the detection and treatment of human adenovirus (HAdVs) disseminated disease in the case of a 46-year-old immunocompromised female having myelodysplastic syndrome with refractory cytopenia with multilineage dysplasia: International Prognostic Scoring System 1. Serum and urine samples were tested for the presence of adenoviral DNA using the quantitative real-time polymerase chain reaction (PCR) assay. For additional confirmation, sequencing of PCR products was also performed. With real-time PCR, we detected HAdV DNA in both serum and urine samples. The viral level constantly decreased with applied oral ribavirin therapy. As the result of sequencing, HAdVs type 11 was determined. Surveillance of adenovirus by real-time PCR is useful in detecting and monitoring disseminated HAdV infection; it is a potential standard diagnostic approach that could assist clinicians to decide whether antiviral therapy ought to be administered.
    Archivum Immunologiae et Therapiae Experimentalis 10/2011; 59(6):473-7. · 2.38 Impact Factor
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    ABSTRACT: In patients with immunological disorders, adenovirus infections are associated with significant rates of morbidity and mortality. Only few hematological units use molecular virological methods, such as polymerase chain reaction, for surveillance of adenovirus infection, and treatment strategies have never been evaluated in multicenter clinical trials. This report describes the detection and treatment of human adenovirus (HAdVs) disseminated disease in the case of a 46-year-old immunocompromised female having myelodysplastic syndrome with refractory cytopenia with multilineage dysplasia: International Prognostic Scoring System 1. Serum and urine samples were tested for the presence of adenoviral DNA using the quantitative real-time polymerase chain reaction (PCR) assay. For additional confirmation, sequencing of PCR products was also performed. With real-time PCR, we detected HAdV DNA in both serum and urine samples. The viral level constantly decreased with applied oral ribavirin therapy. As the result of sequencing, HAdVs type 11 was determined. Surveillance of adenovirus by real-time PCR is useful in detecting and monitoring disseminated HAdV infection; it is a potential standard diagnostic approach that could assist clinicians to decide whether antiviral therapy ought to be administered.
    Arch Immunol Ther Exp (Warsz). 01/2011;
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    ABSTRACT: Hemophagocytic lymphohistiocytosis (HLH) is a rare (especially in adults) syndrome in which hyperinflammation leads to fever, hepatosplenomegaly, pancytopenia and when untreated leads to death. Characteristic laboratory findings include: hyperferritinemia, hypertrigliceridemia, hypofibrinogenemia, hemophagocytosis, low/absent NK-cell activity and high serum sCD25 levels. HLH in adults is in most cases secondary to viral infection (with an Epstein-Barr virus predominacy). A very rare case of T-cell lymphoma-induced HLH in an 80 year old patient is reported. This diagnosis was made when patient was already in critical condition and although after introducing treatment according to the HLH-2004 protocol (etoposide, cyclosporine A, corticosteroids) signs of improvement were observed, the outcome was fatal. Described case shows usefulness of more frequent ferritin concentration measurements in similar patients. Extremely high (> 10 000 μg/l) hyperferritinemia is a hallmark of HLH and may enable control of this syndrome which is 100% fatal without treatment. Full text available at: http://6096.indexcopernicus.com//fulltxt.php?ICID=971600
    Oncoreview. 01/2011;
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    ABSTRACT: Epstein-Barr virus (EBV) is the major triggering factor for hemophagocytic syndrome or hemophagocytic lymphohistiocytosis (HLH). In patients with EBV-HLH, the EBV-infected T cells or natural killer cells are mostly mono- or oligoclonally proliferating, whereby hypercytokinemia plays a major role and causes hemophagocytosis, cellular damage, and dysfunction of various organs. This report describes the detection and treatment of EBV-associated HLH in the case of a 17-year-old male. Serum samples and skin swabs were tested for the presence of viral DNA using real-time PCR techniques. To confirm the molecular biological tests, electron microscopy was also performed. EBV DNA was detected with real-time PCR in both blood samples and skin swabs. The level of viral DNA constantly decreased during the applied therapy. The presence of the virus in the skin was confirmed by the appearance of herpes virus-like particles detected by electron microscopy in fluid taken from skin ulcerations. The results show that in terms of treatment, special therapeutic measures are required to control the cytokine storm generated by EBV and to suppress proliferating EBV genome-containing cells because the clinical course is often fulminate and results in a poor outcome. Therefore the potential of chemotherapy with a combination of steroids, etoposide, and cyclosporine to control HLH was assessed in the adolescent, who met the stringent diagnostic criteria for this reactive disorder of the mononuclear phagocyte system.
    Archivum Immunologiae et Therapiae Experimentalis 04/2010; 58(3):247-52. · 2.38 Impact Factor