Publications (3)2.67 Total impact
Article: Diagnostic application of serum proteomic patterns in early gastric cancer patients by SELDI-TOF-MS[show abstract] [hide abstract]
ABSTRACT: ObjectiveSurface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) is one of the currently used techniques to identify biomarkers for cancers. This study was planned to make a proteomic analysis on the serum of stage I gastric cancer patients and establish a early diagnostic model for identifying stage I gastric cancer preliminarily. MethodsA total of 229 serum samples including 169 pathologically confirmed gastric cancer patients (stage I: n = 47; stage II: n = 39; stage III: n = 46; stage IV: n = 37) and 60 gastritis patients were analyzed by SELDI-TOF-MS Protein Chip array technology. The resulting SELDI-TOF-MS spectral data were analyzed using the Biomarker Wizard™ and Biomarker Patterns™ software to find differential proteins and develop a classification tree for early gastric cancer. ResultsTwelve proteins were significantly over expressed (P < 0.05, Student t-test) in patients with gastric cancer compared to patients with gastritis. Among them, three protein masses with 2873, 6121 and 7778 m/z was found to be differentially expressed solely in patients suffering from stage I gastric cancer. The three protein masses construct the decision tree which could effectively distinguish stage I gastric cancer samples from control samples, achieving a sensitivity and specificity of 89.36 (42/47) and 97.32% (182/187), respectively. ConclusionSerum Proteomic Patterns can be used for the quick diagnosis of stage I gastric cancer. SELDI-TOF-MS is a useful tool for the detection and identification of new protein markers in serum. Key wordsgastric cancer–biomarkers–SELDI-TOF-MS–serum proteomic patternsThe Chinese-German Journal of Clinical Oncology 04/2012; 10(5):261-265.
Article: Interleukin-10 gene polymorphisms influence susceptibility to cachexia in patients with low-third gastric cancer in a Chinese population.[show abstract] [hide abstract]
ABSTRACT: Interleukin (IL)-10 is a pleiotropic cytokine that can both stimulate and suppress the immune response. Previous studies have reported that IL-10 production was significantly elevated in cachectic patients, and it has been confirmed that polymorphisms of the IL10 gene could influence its expression. Therefore, we designed this study to investigate whether polymorphisms of the IL10 gene were associated with cachexia in patients with low-third gastric cancer in a Chinese population. 190 patients with low-third gastric cancer were included in this study. The serum levels of IL-10 were measured by radioimmunoassay. The single nucleotide polymorphisms (SNPs) at positions -1082A/G, -819T/C, and -592A/C in the IL10 gene promoter were analyzed using polymerase chain reaction (PCR) restriction fragment length polymorphism (PCR-RFLP). The serum levels of IL-10 were significantly higher in patients with cachexia than in those without (Z = -10.66, p < 0.001). Single SNP analysis showed that the frequency of the IL10 -1082G allele was increased in patients with cachexia (p = 0.02). The -1082AG and -819CC genotypes were observed to be associated with an increased risk of cachexia. In a logistic regression analysis adjusted for actual weight and carcinoma stage, the -1082AG genotype was associated with an odds ratio (OR) of 2.45 (95% CI 1.21, 4.96; p = 0.01), and the -819CC genotype was associated with an OR of 3.70 (95% CI 1.20, 11.39; p = 0.02) for cachexia. Furthermore, haplotype analysis of the -1082A/G, -819T/C, and -592A/C SNPs revealed that at least five haplotypes (ATA, ACC, GCC, ACA, and ATC) were present in this Chinese population, and the -1082G/-819C/-592C (GCC) haplotype was associated with a significantly increased risk of cachexia as compared with the ATA haplotype (OR = 2.42; 95% CI 1.17, 5.00; p = 0.02). Our results indicate that genetic polymorphisms of IL-10 may influence susceptibility to cachexia in patients with low-third gastric cancer in this Chinese population.Molecular diagnosis & therapy 04/2010; 14(2):95-100. · 1.71 Impact Factor
Article: Association of interleukin-10 gene polymorphism with cachexia in Chinese patients with gastric cancer.[show abstract] [hide abstract]
ABSTRACT: This study investigated whether the single nucleotide polymorphisms (SNPs) and haplotypes of interleukin-10 (IL-10) were associated with cachexia in 223 Chinese patients with gastric cancer diagnosed by histopathological examination. Genomic DNA was extracted from peripheral blood leukocytes. The SNPs at positions -1082A/G, -819T/C, and -592A/C in the IL-10 gene promoter were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). No significant differences were found in the allele and genotype frequencies of -592A/C in patients with or without cachexia. Increased frequency of the -1082G allele was found in patients with cachexia (OR = 1.83, 95% CI, 1.00-3.33, p = 0.049). In a logistic regression analysis adjusted for body weight, carcinoma location, and stage, the -1082AG genotype was associated with an odds ratio of 1.989 (95% CI, 1.041-3.802, p = 0.037) for cachexia. The -819CC genotype was associated with an odds ratio of 3.393 (95% CI, 1.298-8.871, p = 0.013) for cachexia. Furthermore, haplotype analysis revealed that the G(1082)C(819)C(592) haplotype was associated with increased risk of cachexia as compared to the A(1082)T(819)A(592) haplotype (OR = 2.21; 95% CI, 1.14 - 4.30; p = 0.02). Our results suggest that genetic polymorphisms of IL-10 contribute to the susceptibility to cachexia in patients with gastric cancer in the Chinese population.Annals of clinical and laboratory science 01/2010; 40(2):149-55. · 0.96 Impact Factor