Shuangchen Chen

Publications (3)10.17 Total impact

• Article: Role of brassinosteroids in alleviation of phenanthrene-cadmium co-contamination-induced photosynthetic inhibition and oxidative stress in tomato.

  Golam Jalal Ahammed, Sikander Pal Choudhary, Shuangchen Chen, Xiaojian Xia, Kai Shi, Yanhong Zhou, Jingquan Yu
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  ABSTRACT: Heavy metal pollution often occurs together with organic contaminants. Brassinosteroids (BRs) induce plant tolerance to several abiotic stresses, including phenanthrene (PHE) and cadmium (Cd) stress. However, the role of BRs in PHE+Cd co-contamination-induced stress amelioration is unknown. Here, the interactive effects of PHE, Cd, and 24-epibrassinolide (EBR; a biologically active BR) were investigated in tomato plants. The application of Cd (100 µM) alone was more phytotoxic than PHE applied alone (100 µM); however, their combined application resulted in slightly improved photosynthetic activity and pigment content compared with Cd alone after a 40 d exposure. Accumulation of reactive oxygen species and membrane lipid peroxidation were induced by PHE and/or Cd; however, the differences in effect were insignificant between Cd and PHE+Cd. The foliar application of EBR (0.1 µM) to PHE- and/or Cd-stressed plants alleviated photosynthetic inhibition and oxidative stress by causing enhancement of the activity of the enzymes and related transcript levels of the antioxidant system, secondary metabolism, and the xenobiotic detoxification system. Additionally, PHE and/or Cd residues were significantly decreased in both the leaves and roots after application of EBR, more specifically in PHE+Cd-stressed plants when treated with EBR, indicating a possible improvement in detoxification of these pollutants. The findings thus suggest a potential interaction of EBR and PHE for Cd stress alleviation. These results advocate a positive role for EBR in reducing pollutant residues for food safety and also strengthening phytoremediation.
  Journal of Experimental Botany 11/2012; · 5.36 Impact Factor
• Article: Alleviation of chilling-induced oxidative damage by salicylic acid pretreatment and related gene expression in eggplant seedlings

  Shuangchen Chen, Liu Zimei, Jinxia Cui, Ding Jiangang, Xiaojian Xia, Dilin Liu, Jingquan Yu
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  ABSTRACT: The effects of salicylic acid (SA) pretreatment on ascorbate–glutathione (GSH) cycle under chilling stress in eggplant seedlings were investigated. Salicylic acid pretreatment improved chilling resistance of eggplant seedlings with maximum efficiency at a concentration of 0.3%. The chilling injury index was decreased by 36.9% as compared to that of the control after 0.3% SA pretreatment. Under chilling stress, seedlings pretreated with SA displayed lower hydrogen peroxide (H2O2) content and higher ascorbate peroxidase (APX) activity than the untreated seedlings. Pretreatment with SA increased ascorbic acid and GSH content. Salicylic acid (0.3%) pretreatment enhanced the dehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) activity but not glutathione reductase (GR). Pretreatment with SA also enhanced the induced expression of stress-responsive genes under chilling stress. The highest transcript levels of GST1, GST2, GPX1, GPX2, GSH, MDHAR, GR and DHAR upon pretreatment with SA (0.3%) were 6.21, 6.24, 7.76, 5.99, 6.54, 6.44, 2.86 and 2.15 folds, respectively as compared to non-SA-treated seedlings. Taken together, SA pre-treatment could effectively protect eggplant seedlings from oxidative damage of chilling stress through enhancing antioxidant enzymes activities and related gene expression. KeywordsAntioxidant system–Chilling stress–Gene expression–Salicylic acid
  Plant Growth Regulation 05/2012; 65(1):101-108. · 1.60 Impact Factor
• Article: The different responses of glutathione-dependent detoxification pathway to fungicide chlorothalonil and carbendazim in tomato leaves.

  Jitao Wang, Yuping Jiang, Shuangchen Chen, Xiaojian Xia, Kai Shi, Yanhong Zhou, Yunlong Yu, Jingquan Yu
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  ABSTRACT: Chlorothalonil (CHT) and carbendazim (CAR) are two widely used fungicides in agriculture. Despite their agronomic importance in pest control, little is known about their detoxification in the plant. In this study, we investigated the effects of these fungicides on glutathione (GSH) content, GSH-dependent enzyme activities and gene expression in tomato leaves. Results showed that exposure to CHT resulted in increases in GSH content, activities of glutathione S-transferases (GSTs) and glutathione reductase (GR), as well as the transcriptional levels of glutathione S-transferase genes (GST1, GST2 and GST3), glutathione synthetase gene (GSH), glutathione reductase gene (GR) and glutathione peroxidase gene (GPX) in tomato leaves, but such increases were not observed in leaves exposed to CAR. In addition, GSTs, GR, peroxidase (POD) activities and most of GSH-dependent gene expression were induced by CHT in a concentration- and time-dependent manner. These results suggest that GSH-dependent pathway plays an important role in the CHT detoxification but not in the CAR detoxification in tomato leaves.
  Chemosphere 03/2010; 79(9):958-65. · 3.21 Impact Factor