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Publications (3)6.67 Total impact

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    ABSTRACT: Tumor-associated macrophages (TAMs) play an important role in growth, progression and metastasis of tumors. In non-small cell lung cancer (NSCLC), TAMs' anti-tumor or pro-tumor role is not determined. Macrophages are polarized into M1 (with anti-tumor function) and M2 (with pro-tumor function) forms. This study was conducted to determine whether the M1 and M2 macrophage densities in NSCLC are associated with patient's survival time. Fifty patients with an average of 1-year survival (short survival group) and 50 patients with an average of 5-year survival (long survival group) were included in this retrospective study. Paraffin-embedded NSCLC specimens and their clinicopathological data including up to 8-year follow-up information were used. Immunohistochemical double-staining of CD68/HLA-DR (markers for M1 macrophages) and CD68/CD163 (markers for M2 macrophages) was performed and evaluated in a blinded fashion. The M1 and M2 macrophage densities in the tumor islets, stroma, or islets and stroma were determined using computer-aided microscopy. Correlation of the macrophage densities and patient's survival time was analyzed using the Statistical Package for the Social Sciences. Approximately 70% of TAMs were M2 macrophages and the remaining 30% were M1 macrophages in NSCLC. The M2 macrophage densities (approximately 78 to 113 per mm2) in the tumor islets, stroma, or islets and stroma were not significantly different between the long survival and short survival groups. The M1 macrophage densities in the tumor islets (approximately 70/mm2) and stroma (approximately 34/mm2) of the long survival group were significantly higher than the M1 macrophage densities in the tumor islets (approximately 7/mm2) and stroma (13/mm2) of the short survival group (P < 0.001 and P < 0.05, respectively). The M2 macrophage densities were not associated with patient's survival time. The M1 macrophage densities in the tumor islets, stroma, or islets and stroma were positively associated with patient's survival time in a univariate analysis (P < 0.01 or 0.001). In a multivariate Cox proportional hazards analysis, the M1 macrophage density in the tumor islets was an independent predictor of patient's survival time. The M1 macrophage density in the tumor islets is an independent predictor of survival time in NSCLC patients.
    BMC Cancer 03/2010; 10:112. · 3.33 Impact Factor
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    ABSTRACT: Tumor microenvironment is composed of tumor cells, fibroblasts, endothelial cells, and infiltrating immune cells. Tumor-associated immune cells may inhibit or promote tumor growth and progression. This study was conducted to determine whether the number and microlocalization of macrophages, mature dendritic cells and cytotoxic T cells in non-small cell lung cancer are associated with patient's survival time. Ninety-nine patients with non-small cell lung cancer (NSCLC) were included in this retrospective study. Paraffin-embedded NSCLC specimens and their clinicopathological data including up to 8-year follow-up information were used. Immunohistochemical staining for CD68 (marker for macrophages), CD83 (marker for mature dendritic cells), and CD8 (marker for cytotoxic T cells) was performed and evaluated in a blinded fashion. The numbers of immune cells in tumor islets and stroma, tumor islets, or tumor stroma were counted under a microscope. Correlation of the cell numbers and patient's survival time was analyzed using the Statistical Package for the Social Sciences (version 13.0). The numbers of macrophages, mature dendritic cells and cytotoxic T cells were significantly more in the tumor stroma than in the tumor islets. The number of macrophages in the tumor islets was positively associated with patient's survival time, whereas the number of macrophages in the tumor stroma was negatively associated with patient's survival time in both univariate and multivariate analyses. The number of mature dendritic cells in the tumor islets and stroma, tumor islets only, or tumor stroma only was positively associated with patient's survival time in a univariate analysis but not in a multivariate analysis. The number of cytotoxic T cells in the tumor islets and stroma was positively associated with patient's survival time in a univariate analysis but not in a multivariate analysis. The number of cytotoxic T cells in the tumor islets only or stroma only was not associated with patient's survival time. The number of macrophages in the tumor islets or stroma is an independent predictor of survival time in NSCLC patients. Counting macrophages in the tumor islets or stroma is more useful in predicting patient's survival time than counting mature dendritic cells or cytotoxic T cells.
    BMC Cancer 01/2010; 10:220. · 3.33 Impact Factor
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    ABSTRACT: It has been proven that ezrin protein may interact with E-cadherin protein and take part in metastasis of tumors. The aim of this study is to detect the expression of ezrin and E-cadherin and their significance in non-small cell lung cancer (NSCLC) with tissue microarray technique. Ezrin and E-cadherin proteins were detected in 25 cases of benign pulmonary tissues, 287 cases of NSCLC tissues and 120 cases of metastatic lymph nodes by LSAB method of immunohistochemical staining. All patients were followed up. The overexpression rate of ezrin in primary NSCLC tissues and metastatic lymph nodes was 57.8% and 83.3% respectively (P=0.000). The abnormal expression rate of E-cadherin in primary NSCLC tissues and metastatic lymph nodes was 82.6% and 98.3% respectively (P=0.000). The overexpression rate of ezrin was significantly related to grading (P=0.005) and metastasis (P=0.032). The abnormal expression rate of E-cadherin was closely related to grading (P=0.024), metastasis (P=0.015) and TNM stages (P=0.037). There was a negative correlation between expression of ezrin and E-cadherin (P=0.029). Grading, metastasis of NSCLC, TNM stages, overexpression of ezrin and abnormal expression of E-cadherin were independent prognostic factors of NSCLC (P < 0.05). Overexpression of ezrin and abnormal expression of E-cadherin may promote tumor metastasis. Ezrin and E-cadherin may be useful prognostic markers for patients with advanced NSCLC.
    Zhongguo fei ai za zhi = Chinese journal of lung cancer 06/2007; 10(3):183-7.