M Yindee

Publications (5)5.12 Total impact

• Article: Maturation competence of swamp buffalo oocytes obtained by ovum pick-up and from slaughterhouse ovaries.

  M Yindee, M Techakumphu, C Lohachit, S Sirivaidyapong, A Na-Chiangmai, B A J Roelen, B Colenbrander
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  ABSTRACT: This study was designed with the final goal of improving in vitro embryo production in the Thai swamp buffalo (Bubalus bubalis carabensis). Oocytes were collected by ovum pick-up (OPU) from six non-lactating multiparous swamp buffalo twice per week for 10 consecutive sessions followed by once-weekly collection for 10 consecutive sessions without hormone stimulation. In addition, oocytes were collected from slaughterhouse ovaries that were classified as follows: ovaries from non-pregnant cows with a visible corpus luteum (NPCL); pregnant cows with a corpus luteum (P); and non-pregnant cows without a corpus luteum (NP). Follicles in each group of ovaries were categorized as small (2-4 mm), medium-sized (5-8 mm) or large follicles (≥ 9 mm). The quality of the oocytes was assessed by their capacity to undergo in vitro maturation. The total number of observed follicles per session (all sizes combined) was larger in the once-weekly OPU group compared with the twice-weekly OPU group. In particular, the numbers of small and large follicles were higher in the once-weekly OPU group (5.2 ± 0.7 and 0.9 ± 0.2, respectively) than in the twice-weekly OPU group (3.9 ± 0.5 and 0.5 ± 0.1). The number of medium-sized follicles did not differ between the groups. The percentages of oocytes with an abnormal spindle morphology were not different between oocytes from the twice-weekly (30.0%) and the once-weekly (28.6%) OPU groups. A higher percentage of oocytes obtained in vitro (49.5%) exhibited nuclear abnormalities compared with those obtained in vivo (≤34.8%) after in vitro maturation. In conclusion, oocytes can be successfully collected by OPU in the swamp buffalo, without hormonal pretreatment, and per week more good-quality oocytes can be collected by twice-weekly OPU. In addition, oocytes collected from slaughterhouse ovaries can be used with the reproductive status of the cow having no influence on the maturation competence of oocytes.
  Reproduction in Domestic Animals 07/2011; 46(5):824-31. · 1.36 Impact Factor
• Source

  Available from: Heriberto Rodriguez-Martinez

  Article: Follicular dynamics and oestrous detection in Thai postpartum swamp buffaloes (Bubalus bubalis).

  M Yindee, M Techakumphu, C Lohachit, S Sirivaidyapong, A Na-Chiangmai, H Rodriguez-Martinez, G C van der Weyden, B Colenbrander
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  ABSTRACT: This study characterized follicular activity and oestrous behaviour from 5 to 9 days post-calving up to the 4th ovulation postpartum (pp) in 16 multiparous (range 2-7 parities) Thai swamp buffalo cows (Bubalus bubalis), aged 4-12 years and weighing from 432 to 676 kg. Ovarian follicular activity was examined by transrectal ultrasonography (TUS) every morning. Oestrous detection was performed twice daily by direct personal observation of behaviour and for presence of clear cervical mucus discharge and indirectly by video camera recording during 21 h/day. A follicular wave-like pattern was present before the 1st ovulation leading to short oestrous cycles. Growth rates and maximum diameters of the ovulatory follicles did not differ between the 1st and 4th ovulations. However, growth rate for non-ovulatory dominant follicles (DF) before the 1st ovulation was lower than for the ovulatory follicle (p<0.05). In addition, the diameter of all ovulatory follicles (14.3 ± 0.46 mm, n=39) was significantly larger (p < 0.01) than those of the preceding last but one non-ovulatory DF (10.8 ± 0.20 mm, n = 5), but similar to the last preceding non-ovulatory DF diameter (12.92 ± 0.96 mm, n = 14). Short oestrous cycles were most common between the 1st and 2nd ovulations (93.75%, 15/16 cows, 10.2 ± 0.38 days) decreasing in prevalence thereafter (50%, 3/6 buffaloes, 12.0 ± 1.53 days). Oestrous signs were relatively vague around the 1st ovulation pp to become more easily detectable thereafter. This study suggests that properly fed swamp buffaloes could be mated successfully within 2 months pp, at their 2nd spontaneous ovulation, provided oestrous detection is at least performed daily at 06:00-08:00 hour.
  Reproduction in Domestic Animals 02/2011; 46(1):e91-6. · 1.36 Impact Factor
• Source

  Available from: Johannes A Lenstra

  Article: Y-chromosomal variation confirms independent domestications of swamp and river buffalo.

  M Yindee, B H Vlamings, W Wajjwalku, M Techakumphu, C Lohachit, S Sirivaidyapong, C Thitaram, A A A W K Amarasinghe, P A B D A Alexander, B Colenbrander, J A Lenstra
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  ABSTRACT: Y-chromosomal variation in the water buffalo was analysed by sequencing of DBY, ZFY and SRY gene segments. A clear separation of the paternal lineages of the river and swamp types parallels the differences between their maternal lineages and nuclear DNA. Sequence divergence was found to be comparable to the divergence of taurine cattle and zebu, and this divergence predated domestication, confirming that river and swamp buffalo originated from different wild populations. Within a sample of 23 Thai swamp buffaloes, we identified four haplotypes with different geographical distributions, two of which were shared by Thai wild buffaloes.
  Animal Genetics 03/2010; 41(4):433-5. · 2.40 Impact Factor
• Article: Reproduction and genetic diversity of the swamp buffalo

  M. Yindee
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  ABSTRACT: The water buffalo is one of the most important domestic animals in Southeast Asia including Thailand. As the Thai swamp buffalo population declined during the last two decades, the swamp buffalo reproductive performance needs to be improved. Lack of knowledge on swamp buffalo reproduction, improper management and failure to use genetic superior males and females in breeding programs are the major factors to be considered. Artificial insemination was applied in Thailand but is inefficient due to suboptimal heat detection. Our study emphasized that the presence of a buffalo teaser bull is a very helpful tool for heat detection in the female swamp buffalo and thus for the proper insemination time either natural or artificial. There are also other signs of estrus of the female buffalo which can be detected by trained experts. Postpartum anestrus is one of the major causes of poor reproductive performance in the buffalo leading to the long period of days open and thus a long calving interval. To improve postpartum buffalo reproduction efficiency, proper husbandry management such as quality of nutrition, optimal estrus detection and a fertile buffalo bull are crucial. In this study, the postpartum buffalo could be successfully mated within two months postpartum at the second spontaneous ovulation, even without hormonal treatment. Embryo transfer would allow a wider exploitation and dissemination of superior swamp buffalo genotypes. We demonstrated that oocytes for IVM and IVF can be collected successfully by OPU without hormonal pretreatment, and those twice weekly pick-up resulted in more oocytes than once-weekly pick-up. In order to reinforce a more effective management of buffalo genetic resources and reconstruct their genetic history, we studied the genetic diversity of both paternal and maternal lineages. We found evidence for a separate domestication of the river and swamp buffalo, a shared wild-ancestral origin of the swamp buffalo A and B mtDNA haplotype and a Southeast Asian domestication site of the swamp buffalo. Occurrence of Y-chromosomal DNA variants depends on the region within Thailand, indicating a restricted migration of water buffaloes. Our data warrant a wider investigation of swamp buffalo mtDNA and Y-chromosomal diversity as well as a comprehensive study of autosomal variation. Insight in the genetic constitution of water buffalo may prove essential for the preservation and utilization of the genetic resources of Thailand
• Article: Y-chromosomal variation confirms independent domestications of swamp and river buffalo.

  M Yindee, B.H. Vlamings, W. Wajjwalku, M Techakumphu, C Lohachit, S Sirivaidyapong, C. Thitaram, A.A. Amarasinghe, P.A. Alexander, B Colenbrander, J A Lenstra
  [show abstract] [hide abstract]
  ABSTRACT: Y-chromosomal variation in the water buffalo was analysed by sequencing of DBY, ZFY and SRY gene segments. A clear separation of the paternal lineages of the river and swamp types parallels the differences between their maternal lineages and nuclear DNA. Sequence divergence was found to be comparable to the divergence of taurine cattle and zebu, and this divergence predated domestication, confirming that river and swamp buffalo originated from different wild populations. Within a sample of 23 Thai swamp buffaloes, we identified four haplotypes with different geographical distributions, two of which were shared by Thai wild buffaloes.