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Jundong Gu,
Yanjun Wen, Siwei Zhu,
Feng Hua,
Hui Zhao,
Hongrui Xu,
Jiacong You,
Linlin Sun,
Weiqiang Wang,
Jun Chen,
Qinghua Zhou
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ABSTRACT: Aberrant methylation of CpG islands acquired in tumor cells in promoter regions plays an important role in carcinogenesis. Accumulated evidence demonstrates P(16INK4a) gene promoter hypermethylation is involved in non-small cell lung carcinoma (NSCLC), indicating it may be a potential biomarker for this disease. The aim of this study is to evaluate the frequency of P(16INK4a) gene promoter methylation between cancer tissue and autologous controls by summarizing published studies.
By searching Medline, EMBSE and CNKI databases, the open published studies about P(16INK4a) gene promoter methylation and NSCLC were identified using a systematic search strategy. The pooled odds of P(16INK4A) promoter methylation in lung cancer tissue versus autologous controls were calculated by meta-analysis method.
Thirty-four studies, including 2 652 NSCLC patients with 5 175 samples were included in this meta-analysis. Generally, the frequency of P(16INK4A) promoter methylation ranged from 17% to 80% (median 44%) in the lung cancer tissue and 0 to 80% (median 15%) in the autologous controls, which indicated the methylation frequency in cancer tissue was much higher than that in autologous samples. We also find a strong and significant correlation between tumor tissue and autologous controls of P(16INK4A) promoter methylation frequency across studies (Correlation coefficient 0.71, 95% CI:0.51-0.83, P<0.0001). And the pooled odds ratio of P(16INK4A) promoter methylation in cancer tissue was 3.45 (95% CI: 2.63-4.54) compared to controls under random-effect model.
Frequency of P(16INK4a) promoter methylation in cancer tissue was much higher than that in autologous controls, indicating promoter methylation plays an important role in carcinogenesis of the NSCLC. Strong and significant correlation between tumor tissue and autologous samples of P(16INK4A) promoter methylation demonstrated a promising biomarker for NSCLC.
PLoS ONE 01/2013; 8(4):e60107. · 4.09 Impact Factor
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ABSTRACT: We generate and investigate a self-healing pillar array with a specific phase function comprised of four engineered cubic phase plates with encoded amplitude and phase. This array shows an enhanced self-healing effect at arbitrary obstructed positions in transverse as well as longitudinal locations along propagation where its symmetrical pattern with uniform intensity distribution can be maintained. The transverse power flow in terms of Poynting vector is studied for better interpretation of the self-healing property.
Optics Letters 09/2012; 37(17):3540-2. · 3.40 Impact Factor
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ABSTRACT: A novel phase-sensitive surface plasmon resonance (pSPR) biosensor based on differential phase measurement between two cylindrical vector beams, namely radially polarized and azmuthally polarized beams, is proposed and studied in an inverted microscope. Different from a fixed angle or a relatively small angular range for SPR excitation in the attenuated total reflection (ATR) configuration, the signal beam focused by a total internal reflection fluorescence microscopic objective contains the entire angular range from 0 to the maximum angle given by the numerical aperture, leading to a dynamic range of 0.41 RIU which is over seven times wider than the best result of the ATR pSPR sensor. Moreover, with the technique of differential phase measurement between radial and azimuthal polarizations employed in our configuration, high sensitivity of ±9.05×10(-8) refractive index unit/1 deg can simultaneously be achieved in principle. The proposed technique maintains the unique advantages in terms of securing high imaging resolution and sensitivity with an ultra-wide dynamic range simultaneously.
Optics Letters 06/2012; 37(11):2091-3. · 3.40 Impact Factor
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ABSTRACT: A dynamic optical tweezers system is employed for generation of an optical trap in continuous rotation for manipulating a biological cell in an aqueous solution. When the rotating speed is increased, the trapped cell experiences an augmented viscous drag force, and eventually it escapes from the trap at the critical rotating speed: when the drag force is greater than the trapping force. With experimental verifications, the method can easily be employed to differentiate cells in terms of trapping forces due to different refractive indices. The proposed method is a simple, robust, accurate and noninvasive label-free technique for cell detection.
The Review of scientific instruments 06/2011; 82(6):064301. · 1.52 Impact Factor
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ABSTRACT: It has been reported that there was a close relationship between lung cancer risk and environmental tobacco smoke at workplace. The aim of this study is to explore the relationship between workplace environmental tobacco smoke exposure and lung cancer risk among non-smoking subjects.
By searching Medline, CENTRAL (the Cochrane central register of controlled trials), EMBASE, CBM, CNKI and VIP et al, we collected both domestic and overseas published documents on workplace environmental tobacco smoke exposure and lung cancer risk. Random or fixed effect models were applied to conduct systematic review on the study results, the combined odds ratio (OR) and the 95% confidence interval (CI) were calculated as well.
22 reports were included into the combined analysis, which indicated that 25% lung cancer risk was increased by exposing to workplace environment tobacco smoke (OR=1.25, 95%CI: 1.13-1.39, P < 0.001). For female the increased risk was 22% (OR=1.22, 95%CI: 1.05-1.42, P=0.011). For male the increased risk was 54%, but it does not reach the statistical significance (OR=1.54, 95%CI: 0.74-3.18, P=0.247).
Workplace environmental tobacco smoke exposure is an important risk factor of lung cancer risk among non-smoking subjects. Especially for non-smoking women who expose to workplace environment tobacco smoke have a close relationship with lung cancer.
Zhongguo fei ai za zhi = Chinese journal of lung cancer 04/2011; 14(4):345-50.
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ABSTRACT: An on-chip optical trapping and sorting system composed of a microchamber and a microlens array (MLA) is demonstrated. The MLA focuses the incident light into multiple confocal spots to trap the particles within the microchamber. The SiO(2)/ZrO(2) solgel material is introduced in the fabrication of MLA for its unique optical and chemical characters. Moreover, in order to prove the effectiveness of the system, experimental demonstration of multibeam trapping and locked-in transport of micropolymer particles in the microchamber is implemented. The system may easily be integrated as microfluidic devices, offering a simple and efficient solution for optical trapping and sorting of biological particles in lab-on-a-chip technologies.
Applied Optics 01/2011; 50(3):318-22. · 1.41 Impact Factor
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ABSTRACT: cytokeratin-19 fragment (CYFRA21-1) is a soluble protein in serum, and may be a useful circulating tumor marker. The aim of this study is to investigate the diagnostic value of the peripheral blood CYFRA21-1 in non-small cell lung cancer (NSCLC).
the levels of peripheral blood CYFRA21-1 were detected in 107 patients with NSCLC and 51 patients with benign pulmonary diseases by enzyme linked immunosorbent assay, and ROC curve was used to analyse the results.
singificant difference of peripheral blood CYFRA21-1 levels was detected between the NSCLC group and benign pulmonary disease group (Chi-Square=47.343, P < 0.001). At the threshold of 3.3 ng/mL, sensitivity and specificity of CYFRA21-1 as a serologic marker were 74.77% and 76.47%, respectively for any cancer. ROC curve showed that the under-curve area (AUC) of CYFRA21-1 was 0.813 9. There was no significant difference of CYFRA21-1 between subtypes of NSCLC (Chi-Square=0.450, P=0.799). The peripheral blood CYFRA21-1 level was elevated significantly in the patients with extensive disease (IIIb, IV) compared with patients with limited disase (I, II, IIIb) (Chi-Square=7.057, P=0.008).
as a tumor marker CYFRA21-1 has relative high sensitivity and specificity for the diagnosis of NSCLC. Elevated peripheral blood CYFRA 21-1 levels were usually indicated extensive disease of NSCLC.
Zhongguo fei ai za zhi = Chinese journal of lung cancer 12/2010; 13(12):1118-21.
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ABSTRACT: We report a method for microfluidic multiple trapping and continuous sorting of microparticles using an optical potential landscape projected by a Dammann grating, enabling a high power-efficient approach to forming a composite two-dimensional spots array with high uniformity. The Dammann grating is fabricated in a photoresist by optical lithography. It is employed to create an optical lattice for multiple optical trapping and sorting in a mixture of polymer particles (n = 1.59) and silica particles (n = 1.42) with the same diameters of 3.1 μm. In addition to the exponential selectivity by the projected optical landscapes, the proposed microfluidic sorting system has advantages in terms of high power efficiency and high uniformity due to the Dammann grating.
Applied Optics 10/2010; 49(28):5437-43. · 1.41 Impact Factor
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ABSTRACT: We study modified optical vortices for encoding topological charges based on radius ratios between principal and the modified first sidelobe rings. The method is immune to harassments from alignment and phase matching between the beams and optical elements. Moreover, it is demonstrated that the radius ratios and their corresponding modulation parameters are independent to wavelength and size of the spiral phase mask.
Optics Letters 05/2010; 35(9):1485-7. · 3.40 Impact Factor
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ABSTRACT: This paper establishes explicit relations between the radial modulation and the optimal sidelobe expression effect in the Bessel-like modulation technique. It verifies that both central and outer ring areas of the phase plate result in sidelobes in the diffraction pattern, and the corresponding structural dimensions can be determined quantitatively. This approach gives rise to complete sidelobe suppression by taking optimal modulation parameters.
Journal of the Optical Society of America A 04/2010; 27(4):935-40. · 1.56 Impact Factor
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ABSTRACT: ObjectiveTo observe the effects of the new technique of flexible 3D-conformal radiotherapy with combination of photon and electron
(3DCRT) in the treatment of the patients with diffuse malignant pleural mesothelioma (MPM), and carry out the comparative
study between flexible 3DCRT and hemithoracic conventional radiotherapy (CRT).
MethodsFrom January 2004 to October 2007, 8 patients with MPM were treated with flexible 3DCRT. 5 patients had received cycles of
chemotherapy before radiation. New technique of flexible 3DCRT with combination of photon and electron was used in our study,
and DT 32.2–64 Gy with conventional split were delivered. CRT technique was mimicked to compare with 3DCRT technique to predict
the possibility of lung damage in two methods.
ResultsOne patient reached CR and other 7 patients got PR after radiation. Two patients died during the follow-up. The median survival
time (MST) was 15.4 months and it was 18.8 months for sequential chemotherapy and radiotherapy group and 9.7 months for radiotherapy
alone group. The V20, V30, and ipsilateral and contralateral median lung dosage (MLD) were 20.5%, 15.6%, 18.8 Gy and 2.2 Gy
respectively when the flexible 3DCRT technique was used, whereas they were 36.8%, 27.9%, 31.1 Gy and 1.2 Gy respectively when
the CRT technique was used. They were statistically different for the lung V20, V30 and ipsilateral MLD between the two techniques
(P < 0.01), whereas there was no different for the contralateral MLD (P = 0.08). All patients received radiation were found to have lung fibrosis and classified as grades 1–2 radiation pneumonitis.
The quality of life was increased from score 2.83 to 3.76 and it was significantly different (P < 0.01).
ConclusionMPM is moderately sensitive to radiation. The flexible 3DCRT technique is feasible in the treatment of MPM and lung damage
is reduced apparently comparing with the CRT technique. The quality of life of patients with MPM is improved after irradiation.
The Chinese-German Journal of Clinical Oncology 04/2008; 7(5):292-295.
