Z B Simada

Publications (2)4.84 Total impact

• Article: Axonal transport is inhibited by a protein kinase C inhibitor in cultured isolated mouse dorsal root ganglion cells.

  H Hiruma, H Maruyama, T Katakura, Z B Simada, S Nishida, S Hoka, T Takenaka, T Kawakami
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  ABSTRACT: We investigated roles of protein kinase C (PKC) and Ca2+/calmodulin-dependent protein II (CAM II) kinase activities in the maintenance of axonal transport in cultured isolated mouse dorsal root ganglion (DRG) cells. Video-enhanced microscopic recordings revealed that the PKC inhibitor chelerythrine (1 microM) reduced anterograde and retrograde axonal transport, while the CAM II kinase inhibitor KN-62 (10 microM) had no effect. Morphological observation showed that neurite growth was prevented by the presence of chelerythrine (1 microM). From these results, we conclude that PKC activity is required to maintain axonal transport and thereby neurite growth.
  Brain Research 05/1999; 826(1):135-8. · 2.73 Impact Factor
• Article: Lidocaine inhibits neurite growth in mouse dorsal root ganglion cells in culture.

  H Hiruma, H Maruyama, Z B Simada, T Katakura, S Hoka, T Takenaka, T Kawakami
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  ABSTRACT: Recent clinical and experimental studies suggest the effectiveness of lidocaine in blocking neuropathic pain. Because it has been demonstrated that the pathogenetic mechanisms of neuropathic pain involve morphological changes in afferent neuronal terminals onto spinal cord, we examined the effects of lidocaine on neurite growth in isolated mouse dorsal root ganglion cells in culture. Incubation for 2-42 h with various concentrations of lidocaine (0.006 mM, 0.6 mM, and 30 mM) reduced the number of cells exhibiting neurites. The effects were time- and dose-dependent. Lidocaine therefore may exert its pharmacological effect, at least in part, by changing neuronal structures derived from sensory neurons.
  Acta neurobiologiae experimentalis 02/1999; 59(4):323-7. · 2.11 Impact Factor