[Show abstract][Hide abstract] ABSTRACT: Genomic alterations have been identified in lung cancer tissues and reported in numerous studies. To analyze genomic aberrations in lung cancer patients, we used array comparative genomic hybridization (array CGH) in 14 squamous cell lung carcinoma (SqC) tissues. Copy number gain and loss in chromosomal regions were detected, and the corresponding genes were confirmed by real time PCR. Several frequently altered loci, including gain of 3q (36% of samples), were found. The most frequently identified losses were found at 14q32.33 (21% of samples). The relative degree of chromosomal change was analyzed using log2 ratios. High-level DNA amplifications (>0.8 log2 ratio) were detected at 20 regions in 1p, 2q, 3q, 4q, 6q, 7p, 8q, 9p, 10q, 12q, 14q and 19p. We found that the fold change levels were highest at EVI1 (3q26.2), LPP (3q27-28) and FHF-1 (3q28) gene loci. Our results show that array CGH is a useful tool for identification of gene alteration in lung cancer, and that the above-mentioned genes might represent potential candidate genes for pathogenesis and diagnosis of lung cancer.
Lung Cancer 01/2007; 55(1):43-51. DOI:10.1016/j.lungcan.2006.09.018 · 3.96 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Recombinant human bone morphogenic protein-2 (rhBMP-2), which is known as one of the major local stimuli for osteogenic differentiation,
was immobilized on the surface of hyaluronic acid (HA)-modified poly(ε-caprolactone) (PCL) (HA-PCL) scaffolds to improve the
attachment, proliferation, and differentiation of human bone marrow stem cells (hBMSCs) for bone tissue engineering. The rhBMP-2
proteins were directly immobilized onto the HA-modified PCL scaffolds by the chemical grafting the amine groups of proteins
to carboxylic acid groups of HA. The amount of covalently bounded rhBMP-2 was measured to 1.6 pg/mg (rhBMP/HA-PCL scaffold)
by using a sandwich enzyme-linked immunosorbant assay. The rhBMP-2 immobilized HA-modified-PCL scaffold exhibited the good
colonization, by the newly differentiated osteoblasts, with a statistically significant increase of the rhBMP-2 release and
alkaline phosphatase activity as compared with the control groups both PCL and HA-PCL scaffolds. We also found enhanced mineralization
and elevated osteocalcin detection for the rhBMP-2 immobilized HA-PCL scaffolds, in vitro.
Macromolecular Research 10/2006; 14(5):565-572. DOI:10.1007/BF03218725 · 1.60 Impact Factor