-
Biomedical and Environmental Sciences 03/2013; 26(3):226-9. · 1.35 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To provide the evidence for improving the risk assessment and personal protective equipment and techniques to laboratory staff related to Leishmania.
The laboratory biosafety of Leishmania was preliminarily assessed based on the biological background information, potential hazards in experimental activities, the risk analyses of laboratory personnel and other relevant factors.
The risk assessment on laboratory biosafety of Leishmania was helpful for the establishment of the laboratory standard operating procedure, and was helpful for protecting the staff from infection of Leishmania.
The risk assessment on laboratory biosafety is important to the safety of laboratory activity related to Leishmania, and is of a great significance to protect the laboratory staff.
Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control. 06/2012; 24(3):342-4, 363.
-
[show abstract]
[hide abstract]
ABSTRACT: To analyze the epidemiological status of echinococcosis among children in three different zones of Qinghai Province.
B-ultrasound and ELISA were used in the survey to evaluate echinococcus infection among 6 to 15 year old children in the three zones, namely, Qinghai southern plateau, Qilian mountain-Hehuang valley and Chaidamu basin.
The prevalence of echinococcus infection scanned by B-ultrasound and sera positive rate detected by ELISA in children were 1.5% (320/20 730) and 6.5% (1024/15 762) respectively, and the prevalence of cystic echinococcosis and alveolar echinococcosis by ultrasound were 1.0% (206/20730) and 0.6% (114/20730), respectively. The prevalence and sera positive rate were 9.5% (707/7 453) and 2.3% (269/11 618) in Qinghai southern plateau, 3.8%(289/7 544) and 0.6% (50/8 275) in Qilian mountain-Hehuang valley, and 3.7% (28/765) and 0.1% (1/837) in Chaidamu basin, respectively. The southern plateau showed the highest prevalence, with a significant statistical difference among the three areas (P < 0.01). The prevalence of alveolar echinococcosis was 1.0% (114/11 618) in the southern plateau, but no alveolar echinococcosis patients were found in other two areas. Correlation analysis showed that the prevalence increased with the altitude (r(s-e) = 0.96, P < 0.05, R2 = 0.93; r(p-e) = 0.82, P < 0.05, R2 = 0.67).
The prevalence of echinococcosis among children shows an apparent geographical difference in Qinghai Province.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 04/2012; 30(2):127-30.
-
[show abstract]
[hide abstract]
ABSTRACT: The rodentia and lagomorpha animals are the intermediate hosts of Echinococcus multilocularis, their distribution and infection of this parasite may facilitate the infection of definitive hosts such as dogs. The aim of this study was to determine the distribution of the intermediate hosts of Echinococcus multilocularis in Shiqu County, Sichuan, China.
A systematic sampling method was used to investigate the density of burrows of rodents and lagomorphs at 97 pasture sites in winter and summer pastureland and remote sensing (RS) technology was used to correlate their densities to the distribution of these animals in different landscape types.
Based on the densities of Ochotona curzoniae, Microtus fuscus (dependent variable) and their burrow densities (independent variable) in survey points, regression equations were fitted respectively (Ochotona curzoniae, P < 0.0001, R(2) = 0.8705; Microtus fuscus, P < 0.0001, R(2) = 0.9736). Their burrow density in summer pastureland was higher than in winter pastureland (F = 36.65, P < 0.0001). The burrow densities of Ochotona curzoniae and Microtus fuscus in bareland and half-bareland are higher than in grassland (F = 7.73, P < 0.001).
The regression relationship between the densities of Ochotona curzoniae and Microtus fuscus and their burrow densities indicate that the burrow densities could reflect the animal densities and that the burrow density was greater in summer pastureland than in winter pastureland. The main distribution areas of the intermediate hosts were in bareland and half-bareland.
Chinese medical journal 09/2011; 124(18):2834-7. · 0.86 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To clone and express EPCl gene of Echinococcus granulosus, and investigate its immunogenicity and diagnostic value.
Total RNA was extracted from hydatid cyst protoscoleces and EPC1 gene of Echinococcus granulosus was amplified by RT-PCR. The PCR product was cloned into pGEM-T vector, and then subcloned into the prokaryotic expression vector PET28a(+). The positive recombinants were transformed into Escherichia coli BL21 (DE3), and followed by expression of the protein induced by IPTG. The recombinant protein was identified by SDS-PAGE and Western blotting, and used to establish ELISA. Serum samples from patients with cystic echinococcosis (60 cases), alve-olar echinococcosis (37 cases), cysticercosis (16 cases), clonorchiasis sinensis (7 cases), schistosomiasis japonica (4 cases) and healthy persons (33 cases) were examined.
The recombinant plasmid PET28a-EgEPC1 was identified by restriction enzyme digestion and sequencing. SDS-PAGE result showed that the recombinant containing recombinant plasmid PET28a-EgEPC1 expressed a soluble fission protein of EgEPC1 (about M, 11 000). The protein was recognized by pool sera of cystic echinococcosis patients. The overall sensitivity and specificity of diagnosis by ELISA for cystic echinococcosis were 78.3% (47/60), and 98.3% (59/60), respectively. The cross reaction with sera of alveolar echinococcosis was 40.5% (15/37).
The recombinant EgEPC1 antigen has diagnostic value in cystic echinococcosis.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 06/2011; 29(3):167-71.
-
[show abstract]
[hide abstract]
ABSTRACT: To evaluate the predominance, characteristic and shortage of the scientific activities at the National Institute of Parasitic Diseases (NIPD), through analysis on its award-winning research achievements.
Information was collected on the award-winning research achievements by the Institute in the last 45 years. Time trend, disease category, subject, award type, award grade, award level and project property of the award-winning research achievements were analyzed by using SPSS11.0 software.
Totally, 78 research achievements were awarded 128 times in the last 45 years. 43.6% awards were at province and ministry level; 23.1% awards were at nation level. Awards involved in schistosomiasis, malaria, and kala-azar accounted for 33.3%, 28.2% and 12.8%, respectively. As for subject, preventive medicine, pharmacy and biology accounted for 28.2%, 18.0% and 16.7%, respectively; 82.1% of the awards belonged to applied researches.
NIPD has a strong capacity in scientific research. To get more achievements in future, it is essential to closely integrate the experiment research to disease control.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 08/2010; 28(4):287-9.
-
[show abstract]
[hide abstract]
ABSTRACT: Drug resistance in Plasmodium falciparum is a serious public health threat in the countries where this organism is endemic. Since resistance has been associated with specific single-nucleotide polymorphisms (SNPs) in parasite genes, molecular markers are becoming useful surrogates for monitoring the emergence and dispersion of drug resistance. In this study, a multiplex PCR (mPCR) and oligonucleotide microarray method was developed for the detection of these SNPs in genes encoding chloroquine resistance transporter (Pfcrt), multidrug resistance 1 (Pfmdr1), dihydrofolate reductase (Pfdhfr), dihydropteroate synthetase (Pfdhps), and ATPase 6 (PfATPase6) of P. falciparum. The results show that DNA microarray technology, combined with mPCR, is a promising and time-saving tool that supports conventional detection methods, allowing sensitive, accurate, simultaneous analysis of the SNPs associated with drug resistance in P. falciparum.
Journal of clinical microbiology 08/2008; 46(7):2167-74. · 4.16 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To develop a multiplex PCR protocol for amplification of five Plasmodium falciparum drug resistance related genes, thereby facilitate the rapid and high throughput analysis of the drug resistance molecular markers.
Five pairs of primers were designed according to the reference sequences by using Primer Premier 5.0 and Oligo 6.0 software. Drug resistance related genes, including P. falciparum chloroquine resistance transporter (Pfcrt), multi-drug resistance 1 (Pfmdr1), dihydropteroate synthetase (Pfdhps), dihydrofolate reductase (Pfdhfr) and sarco/endoplasmic reticulum Ca2+-ATPase (PfATPase6), were amplified by single-tube multiplex PCR using Hot Start Taq DNA Polymerase among negative controls (P. vivax, P. berghei, P. cynomolgi, Leishmania donovani, Cryptosporidium andersoni), blank control (using H2O as template), as well as P. falciparum laboratory isolates (3D7, Dd2, HB3, FCC1/HN and CMH/YN) and field samples (collected from Yunnan, Hainan of China and Myanmar). After amplification, the PCR products were analyzed by agarose gel electrophoresis. The sequencing results were aligned to the reference sequence using BLAST.
Five expected bands at 315, 437, 514, 594 and 770 bp were obtained with no additional or nonspecific products in P. falciparum laboratory isolates and field samples. The sequencing results were identical with the reference sequence except the polymorphism sites, and exhibited more than 98.5% homology. The multiplex amplification was performed successfully starting from 0.1 ng of DNA template. No band was observed in negative controls and blank control.
The present study establishes a method to amplify five Plasmodium falciparum drug resistance related genes harboring 21 SNPs by one-tube reaction. The multiplex PCR protocol showing high specificity and sensitivity is more convenient and efficient in analyzing the P. falciparum drug resistance molecular markers as compared with traditional nested PCR.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 01/2008; 25(6):451-6.
-
[show abstract]
[hide abstract]
ABSTRACT: To evaluate the point mutations in Pfcrt and Pfmdr1 genes in Plasmodium falciparum isolated from Hainan Province.
Nested polymerase chain reaction and restriction fragment length polymorphism were used to detect the point mutations at codon 76 of Pfcrt and at codon 86, 1246 of Pfmdr1 in P. falciparum isolates. Chloroquine resistance was measured by the in vitro microtest recommended by WHO.
In 36 samples tested, 28 were successfully amplified for Pfcrt, 64.3% of them carried mutant allele at codon 76, 21.4% with wild allele K76 and 14.3% with mixed allele mutation. While for Pfmdr1, 3.4% isolates displayed the 86Y mutation, 89.7% with wild allele N86 and 6.9% with the mixed alleles in 29 isolates which were amplified successfully for N86Y. No point mutation in Pfmdr1 at codon 1246 was found in 13 isolates from the total 36 samples. By the in vitro test, 72.2% (26/36) showed resistance to chloroquine. The 76T and 86Y mutant alleles were present in both in vitro susceptible and resistant isolates. There was a significant difference between susceptible and resistant isolates carrying 76T mutant codon (P < 0.05), but no significant difference was found in Pfmdr1 (P < 0.05).
There is a significant difference of the 76T prevalence in Pfcrt gene between the susceptible isolate and resistant one of P. falciparum to chloroquine in vitro. The Pfcrt 76T may be used as a predictive marker for chloroquine resistance surveillance.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 06/2005; 23(3):135-9.
-
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 05/2005; 23(2):117-20.
-
[show abstract]
[hide abstract]
ABSTRACT: To analyze factors affecting transmission of schistosomiasis japonica in Poyang Lake.
Successive surveillance data for at least three years from 1992 to 1998 at the schistosomiasis endemic administrative villages surrounding Poyang Lake were collected, including the egg positive rate by stool examination, investment on treatment of patients and mollusciciding in snail habitat, risk areas, bovine infection rate. Data on rainfall and temperature were also obtained from the relevant agencies. Step-wised regression method was employed to analyze the data.
The regression model established is statistically significant, R2 equals 0.735, P < 0.01. The accepted variables affecting the transmission of the disease were natural logarithm of human infection rate in last year, risk areas, infection rate of bovine, investment values of niclosamide per risk area and value of praziquantel administered per infection rate.
Chemotherapy and mollusciciding effectively reduced schistosomiasis transmission in Poyang lake region, while the infection rate, risk areas, bovine infection rate still drive the transmission.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 02/2003; 21(3):164-6.
