To apply and evaluate new methods regarding specific gene and antigen detection in plague surveillance program.
1798 samples from natural foci of plague were tested, using internal quality control multiple-polymerase chain reaction, F1 antigen marked by immuno chromatographic assay and enzyme linked immunosorbent assay. Culture of Yersinia pestis and reverse indirect hemagglutination assay were used as reference diagnostic methods.
The overall positive rate of culture on Yersinia pestis together with gene and antigen detection was 7.34%, showing an 16.81% increase when comparing to 6.28% using Yersinia pestis culture method alone. The rate of coincidence was 97.13%.
The new standard being used for specific gene and antigen detection could increase the positive rate of diagnosis on plague.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 06/2007; 28(5):426-9.
To understand the molecular biological characteristics in order to analyse the genetic background of Yersinia pestis in China.
Primary datum on ribotyping, pulsed field gene electrophoresis (PFGE), random amplified polymorphic DNA (RAPD) and insertion sequence (IS) of Yersinia pestis were used and under cluster analysis. Genetic interval and various methods of recognized molecular feature between different strains were evaluated.
Ribotypes the PFGE types seemed to be corresponding. Stains from Microtus fuscus and area in Tibet Zhongba belonged to 7 copy rRNA gene and the genetic interval were the far more with 6 copy rRNA gene stains, and not definite with RAPD, but with many exceptions. The genetic interval between strains were showed by resemble value.
Yersinia pestis in China had its own manifold, particular molecular biological characteristics due to natural barriers, geographical complex, circumstances in Tianshan Mountains and Gandise Mountains areas. Yersinia pestis were limited to separateness, evoluted only in certain areas to form a great many gene types.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 07/2004; 25(6):509-13.
The strains of Yersinia pestis isolated in different period and different natural foci in China were analyzed.
Traditional and molecular biological methods were used. Rhamnose fermentation, rRNA gene copy number, nitrite reduction, and the glycerol fermentation were important characters for typing, and pulse field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) profile could reflect the genetic distance between the strains.
The strains could be divided into 15 genetic types by those 6 characters with each of them covered an isolated geographical territories.
The characters of strains were described; the genetic relationship of different types, their evolution, and the forming and shift of plague natural foci were analyzed.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 12/2003; 24(11):1005-9.