Vincenzo Monteverde

Istituto Zooprofilattico Sperimentale della Sicilia, Palermo, Sicily, Italy

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Publications (10)6.01 Total impact

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    ABSTRACT: The aim of the study was to investigate clock gene expression in Bos taurus and the alteration of that during two pathological conditions, evaluating the daily expression pattern of four clock genes (Per2, Cry2, Bmal1, Clock) in peripheral blood cells. Five healthy cows, five affected by Brucellosis (BR) and five affected by Bovine Viral Diarrhoea-Mucosal Disease (BVD-MD) were housed in indoor stalls under natural spring conditions, blood samples were collected at 4 h intervals over a 24 h period. Statistical analysis showed rhythmic expression of clock genes mRNAs in healthy cows. Cows affected by BR did not show any rhythmic expression of clock genes mRNAs, cows affected by BDV mRNA levels of Bmal1, Clock and Cry2 changed during the day. These findings highlighted that circadian system could be involved in homeostasis alteration and that clock genes could be considerate as regulatory genes or early response genes during inflammation, so, their regulation should be evaluated in health research and treatment.
    Journal of applied biomedicine 01/2014; · 0.98 Impact Factor
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    ABSTRACT: In this study we compared two polymerase chain reaction (PCR) methods using either 16S ribosomal RNA (rRNA) or 23S rRNA gene primers for the detection of different Leptospira interrogans serovars. The performance of these two methods was assessed using DNA extracted from bovine tissues previously inoculated with several bacterial suspensions. PCR was performed on the same tissues before and after the formalin-fixed, paraffin-embedding procedure (FFPE tissues). The 23S rDNA PCR detected all fresh and FFPE positive tissues while the 16S rDNA-based protocol detected primarily the positive fresh tissues. Both methods are specific for pathogenic L. interrogans. The 23S-based PCR method successfully detected Leptospira in four dubious cases of human leptospirosis from archival tissue specimens and one leptospirosis-positive canine specimen. A sensitive method for leptospirosis identification in FFPE tissues would be a useful tool to screen histological specimen archives and gain a better assessment of human leptospirosis prevalence, especially in tropical countries, where large outbreaks can occur following the rainy season.
    Memórias do Instituto Oswaldo Cruz 02/2012; 107(1):85-8. · 1.36 Impact Factor
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    ABSTRACT: Spirocerca lupi (Rudolphi 1809) is a cosmopolitan nematode of dogs and wild carnivores. In the past it has been reported in Italy, mainly in southern regions and in Sicily, where the parasite was observed in foxes in 2005. The parasite typically produces nodular masses in the oesophagus and thoracic aorta. During the 2003-2004 hunting season, the authors investigated a total of 55 foxes (Vulpes vulpes) hunted or killed by car accidents in the provinces of Palermo and Agrigento. All the foxes were subjected to necropsy and 6 (9.16%) had S. lupi nodules located exclusively in the gastric wall. The nature of the nodules was determined by opening them and detecting the nematodes inside, which were identified as S. lupi. Some of the nodules were characterized anatomopathologically and histopathologically. The formation of the parasitic nodule in the stomach only suggests a deviation from the route commonly followed by the nematode to reach the oesophagus, the elective anatomical site for completion of its lifecycle. This survey gives a contribution to the epidemiology of this parasite which is severely outdated in Italy and highlights some distinctive features of the life cycle and parasite migration.
    Polish journal of veterinary sciences 01/2010; 13(3):465-71. · 0.57 Impact Factor
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    ABSTRACT: The aim of this study was to evaluate haematological and haematochemical responses to training and competition in standardbred horses. We investigated the modifications that occur in athletic horses during different workloads of training and after official competitions of 1,600 and 2,000m. The study was carried out on 15 gelding clinically healthy horses. The animals were divided into three groups: group A, group B, and group C. All animals of group A were subjected to two training schedules with different workloads. Group B took part in a 1,600m trot official competition, and group C took part in a 2,000m trot official competition. Blood samples were collected from each animal at rest, after training/competition, and 60min after the end of the physical exercise. On each subject of groups A, B, and C, red blood cell, white blood cell, haemoglobin, haematocrit, and platelets were recorded by means of a multiparametric automatic analyzer, and the concentrations of triglycerides, glucose, creatinine, urea, glutamate pyruvate transaminases, and glutamate oxaloacetate transaminases were assessed with a spectrophotometer. Two-way repeated measures analysis of variance was used to determine significant differences. The results indicated that exercise had a significant effect on haematological and haematochemical parameters, the effect being proportional to the workload.
    Comparative Clinical Pathology 01/2010; 19(1):95-101.
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    ABSTRACT: Piccione, G., Casella, S., Giannetto, C., Monteverde, V. and Ferrantelli, V. 2009. Exercise-induced modifications on haematochemical and electrophoretic parameters during 1600 and 2000 meters trot races in Standardbred horses. J. Appl. Anim. Res., 35: 131–135.The aim of the present study was to evaluate the modifications of some haematochemical and electrophoretic parameters during two different workloads in Standardbred horses. Ten clinically healthy horses were divided into two equal groups, taking part in an official trot race at 1600 or 2000 meters course, respectively. Blood samples were collected from each horse at rest, after warm up, 0, 30 and 60 min after the end of the race. Serum concentrations of albumin, globulins, total proteins, total cholesterol, triglycerides, glucose, hydroxybutyrate, urea, creatinine, creatine kinase (CK), glutamate pyruvate transaminases (GPT) and glutamate oxalacetate transaminases (GOT) were assessed. Exercise increased triglyceride, glucose, creatinine and CK and decreased GOT which tended to reach rest level 60 min after exercise. Globulins were affected by exercise but there was no set pattern.
    Journal of Applied Animal Research 01/2009; 35(2):131-135. · 0.12 Impact Factor
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    ABSTRACT: The authors studied the changes of some haematological parameters during two different workloads in Standardbred horses. Ten horses, clinically healthy, were divided into two groups of five subjects each. The two groups of Standardbred (Group A and Group B) had been separately and specifically trained to take part in the official 1600 and 2000 meters trot races, respectively. On each subject of Group A and Group B, red blood cell (RBC), white blood cell (WBC), platelets (PLT), haemoglobin (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were recorded. Blood samples were collected from each horse at rest, after warm up, before racing, immediately after racing, 30 and 60 min after the end of the race. Twoway repeated measures analysis of variance (ANOVA) was used to determine significant differences. The statistical analysis, the ANOVA followed by the Bonferroni's test, showed that during the experimental period, modifications observed for some haematological parameters are not related to the intensity and the type of exercise; only platelet reactivity is altered by different workload. Our results further confirm the data demonstrated previously.
    Acta Veterinaria. 01/2008;
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    ABSTRACT: Caudal duplication is a rare type of conjoined twinning. The aim of this case report is to describe a case of caudal duplication in a male crossbreed puppy. The puppy was macroscopically and radiographically examined after death. External features included a supernumerary limb projecting from the perineum, complete lack of tail, anal atresia, double penis and absence of scrotal sacs. Internal examination showed anomalies involving the intestine and the urinary system. At x-ray examination, the supernumerary limb showed features similar to that of the normal right hindlimb but showed absence of fibula and tibia and presence of three metatarsi and phalanges. The authors discuss the pathogenic mechanisms of this condition, infrequently reported in veterinary practice, pointing out the importance of embryonic duplications commonly associated with dystocia.
    Journal of Small Animal Practice 08/2007; 48(7):410-3. · 1.18 Impact Factor
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    The Veterinary record 02/2005; 156(4):120-1. · 1.80 Impact Factor
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    ABSTRACT: The existence of daily rhythmicity of serum total proteins, their fractions and some biochemical parameters in healthy cows (group A) and in cows affected by Brucella abortus (group B) was assessed in order to clarify the role of circadian timing processes in healthy and disease in cows. On each subject, blood samples were collected, every 4 h over a 24-h period, starting at 12:00 on day 1 and finishing at 12:00 on day 2, via intravenous cannulas inserted into the jugular vein. On all samples, serum total proteins and their fractions (α1-, α2-, β1-, β2- and γ-globulins), gammaglutamyl transferase (GGT), alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, creatinine and urea were assessed. The application of one-way analysis of variance showed a significant effect of time of day in group A only on creatinine and urea. In group B, a significant effect of the time of day was observed on total proteins, albumin, β-globulins, GGT, creatinine and urea. Daily rhythmicity was exhibited by creatinine and urea in healthy cow. Whereas, daily rhythmicity of total proteins, albumin, β-globulins, GGT, creatinine and urea was observed in cows affected by B. abortus. Our results improve the knowledge about changes of circadian system during pathological events in dairy cow.
    Comparative Clinical Pathology 22(2).
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    ABSTRACT: The aim of this study was to investigate the effects of seasonal variations in Mediterranean area on haematological parameters in ten dairy cows (Bruna breed) kept under natural photoperiod and temperature. On blood samples, collected through an external jugular venipuncture every 30 days for 12 months, haematological profile was performed using an automatic multiparametric analyser for haematology. Red blood cell (RBC), white blood cell, haemoglobin (Hb), haematocrit (Hct), mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, platelets (PLT) and plateletcrit (PCT) were measured. One-way for repeated measure analysis of variance showed a statistically significant effect of time of year (P < 0.05) on RBC, Hb, Hct, PLT and PCT. The majority of the haematological values obtained, comparable to the values considered to be normal for dairy cows, showed that seasonal variations in haematological parameters are related to changes in physical environment with particular emphasis on ambient temperature, relative humidity and temperature–humidity index. These parameters need further investigations in relation to age and sex differences with particular attention to reproductive status.
    Comparative Clinical Pathology 22(4).