Tung-Han Tsai

Publications (6)12.41 Total impact

• Article: Delayed rupture of pre-existing cerebral aneurysm in a young patient with minor head trauma.

  Cheng-Ta Hsieh, En-Yuan Lin, Tung-Han Tsai, Yung-Hsiao Chiang, Da-Tong Ju
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  ABSTRACT: An intracranial saccular aneurysm is not commonly diagnosed in a patient with head injury. We present a patient with a history of minor head trauma and a CT scan of the brain revealing minimal subarachnoid hemorrhage 17 days prior to admission, complaining of severe headache, dysarthria and focal right limb seizures 3 hours prior to admission. A traumatic aneurysm was suspected based on clinical history and radiological findings including hematoma in the falx region on a CT scan of the brain and an aneurysm of the pericallosal artery on magnetic resonance angiography and four-vessel cerebral angiography. However, at craniotomy, an intracranial non-traumatic saccular aneurysm at the bifurcation of the pericallosal artery was found. The patient recovered fully after successful clipping the aneurysm.
  Journal of Clinical Neuroscience 12/2007; 14(11):1120-2. · 1.25 Impact Factor
• Article: Spontaneous spinal epidural hematomas of cervical spine: report of 4 cases and literature review.

  Cheng-Ta Hsieh, Cheng-Fu Chang, En-Yuan Lin, Tung-Han Tsai, Yung-Hsiao Chiang, Da-Tong Ju
  American Journal of Emergency Medicine 11/2006; 24(6):736-40. · 1.98 Impact Factor
• Source

  Available from: bioscience.org

  Article: Gene therapy of focal cerebral ischemia using defective recombinant adeno-associated virus vectors.

  Tung-Han Tsai, Show-Li Chen, Xiao Xiao, Yung-Hsiao Chiang, Yeou-Ping Tsao
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  ABSTRACT: This review presents our experience and results concerning cerebral stroke gene therapy with a rat model subjected to rAAV-vector delivered IL-1ra and GDNF. The methodology involving the production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus and the creation of a tri-vessel ligation model of focal ischemic cerebral stroke in rats are described in detail. Furthermore, a literature review of other viral vectors, murine models of focal cerebral ischemia and candidates for therapeutic transgenes used for cerebral stroke gene therapy are presented. Lastly, the potentials and limitations of stroke gene therapy are discussed adding an analysis of possibilities of future experiment designs.
  Frontiers in Bioscience 02/2006; 11:2061-70. · 3.52 Impact Factor
• Article: Gene treatment of cerebral stroke by rAAV vector delivering IL-1ra in a rat model.

  Tung-Han Tsai, Show-Li Chen, Xiao Xiao, Yung-Hsiao Chiang, Shinn-Zong Lin, Shu-Wen Kuo, Dai-Wei Liu, Yeou-Ping Tsao
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  ABSTRACT: In this study, we injected recombinant adeno-associated virus (rAAV) vectors expressing the interleukin-1 receptor antagonist (rAAV-IL-1ra) into the cortex of rats experiencing transient cerebral ischemia. An accumulation of IL-1ra in cortical tissues of rAAV-IL-1ra-injected animals was confirmed by ELISA. Triphenyltetrazolium chloride (TTC) staining of viable brain tissue revealed that the rAAV-delivered IL-1ra gene could rescue the brain tissues from ischemia-induced injury. Cortical tissues that received rAAV-IL-1ra injections had both significantly smaller total volumes of infarction as well as smaller areas of infarction on each brain slice when compared with the control models. In situ labeling analysis demonstrated significant reduction of apoptotic cells in cortical tissues rescued by rAAV-IL-1ra. Immunohistochemistry staining revealed that the rescued brain tissues contained the same levels of neuronal cells as contralateral undamaged brain tissues. These findings confirmed that the rAAV delivering the IL-1ra gene is a potential therapy for stroke.
  Neuroreport 06/2003; 14(6):803-7. · 1.66 Impact Factor
• Article: Gene therapy for treatment of cerebral ischemia using defective recombinant adeno-associated virus vectors.

  Tung-Han Tsai, Show-Li Chen, Xiao Xiao, Dai-Wei Liu, Yeou-Ping Tsao
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  ABSTRACT: In this review we present our results and experiences in performing gene therapy of cerebral stroke using recombinant adeno-associated virus (rAAV) vectors in a rat model. The methodologies involving the production of AAV vectors, gene transfer to the brain, and a trivessel ligation model of focal ischemic cerebral stroke in rats are described. Furthermore, a brief description of other viral vectors and candidates of therapeutic transgenes used for gene therapy of cerebral stroke are presented. The potential advantages and limitations of stroke gene therapy are also discussed with the intention of outlining the design of more appropriate experiments.
  Methods 11/2002; 28(2):253-8. · 4.01 Impact Factor
• Article: Recombinant Adeno-Associated Virus Vector Expressing Glial Cell Line-Derived Neurotrophic Factor Reduces Ischemia-Induced Damage

  Tung-Han Tsai, Show-Li Chen, Yung-Hsiao Chiang, Shinn-Zong Lin, Hsin-I Ma, Shu-Wen Kuo, Yeou-Ping Tsao
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  ABSTRACT: To explore the potential of using the recombinant adeno-associated viral (rAAV) vector, expressing glial cell line-derived neurotrophic factor (GDNF) as the gene therapy for stroke, we injected rAAV vectors expressing GDNF (rAAV-GDNF) into the cortex of rats which had been experiencing transient bilateral common carotid artery ligation and right middle cerebral artery ligation for 90 min. GDNF levels in cortical tissues of rAAV-GDNF-injected animals were significantly higher than in the control animals injected with rAAV-expressing lacZ (rAAV-lacZ), indicating that rAAV can deliver and express the GDNF gene in cortical tissues. Triphenyltetrazolium chloride tissue stain analysis revealed that the rAAV-delivered GDNF gene could rescue the brain tissues from ischemia-induced injury. Cortical tissues which received rAAV-GDNF injections had both significantly smaller total volumes of infarction and smaller areas of infarction on each brain slice than those which were injected with rAAV-lacZ. An in situ labeling analysis demonstrated significantly less apoptotic cells in cortical tissues rescued by rAAV-GDNF, indicating prevention of apoptosis as the mechanism of cortical cell protection. Moreover, immunohistochemistry staining of Neu-N indicated that the rescued brain tissues contained the same number of Neu-N-positive neuronal cells as contralateral undamaged brain tissues. This provides strong evidence that cortical neuronal cells can be rescued by GDNF gene therapy. Indeed, these findings show that the rAAV is a potential delivery vector of GDNF gene for the therapy of stroke.
  Experimental Neurology.