Tatiana V Kulakovskaya

Publications (7)19.2 Total impact

• Article: Accumulation of phosphate and polyphosphate by Cryptococcus humicola and Saccharomyces cerevisiae in the absence of nitrogen.

  Natalia A Breus, Lubov P Ryazanova, Vladimir V Dmitriev, Tatiana V Kulakovskaya, Igor S Kulaev
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  ABSTRACT: The search for new phosphate-accumulating microorganisms is of interest in connection with the problem of excess phosphate in environment. The ability of some yeast species belonging to ascomycetes and basidiomycetes for phosphate (P (i) ) accumulation in nitrogen-deficient medium was studied. The ascomycetous Saccharomyces cerevisiae and Kuraishia capsulata and basidiomycetous Cryptococcus humicola, Cryptococcus curvatus, and Pseudozyma fusiformata were the best in P (i) removal. The cells of Cryptococcus humicola and S. cerevisiae took up 40% P (i) from the media containing P (i) and glucose (5 and 30 mM, respectively), and up to 80% upon addition of 5 mM MgSO(4) (.) The cells accumulated P (i) mostly in the form of polyphosphate (PolyP). In the presence of Mg(2+) , the content of PolyP with longer average chain length increased in both yeasts; they both had numerous inclusions fluorescing in the yellow region of the spectrum, typical of DAPI-PolyP complexes. Among the yeast species tested, Cryptococcus humicola is a new promising model organisms to study phosphorus removal from the media and biomineralization in microbial cells.
  FEMS Yeast Research 05/2012; 12(6):617-24. · 2.40 Impact Factor
• Source

  Available from: Nikolay E Nifantiev

  Article: Triterpenoid saponins from the roots of Acanthophyllum gypsophiloides Regel.

  Elena A Khatuntseva, Vladimir M Men'shov, Alexander S Shashkov, Yury E Tsvetkov, Rodion N Stepanenko, Raymonda Ya Vlasenko, Elvira E Shults, Genrikh A Tolstikov, Tatjana G Tolstikova, Dimitri S Baev, Vasiliy A Kaledin, Nelli A Popova, Valeriy P Nikolin, Pavel P Laktionov, Anna V Cherepanova, Tatiana V Kulakovskaya, Ekaterina V Kulakovskaya, Nikolay E Nifantiev
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  ABSTRACT: Two new triterpenoid saponins 1 and 2 were isolated from the methanol extract of the roots of Acanthophyllum gypsophiloides Regel. These saponins have quillaic acid or gypsogenin moieties as an aglycon, and both bear similar sets of two oligosaccharide chains, which are 3-O-linked to the triterpenoid part trisaccharide α-L-Arap-(1→3)-[α-D-Galp-(1→2)]-β-D-GlcpA and pentasaccharide β-D-Xylp-(1→3)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-[β-D-Quip-(1→4)]-β-D-Fucp connected through an ester linkage to C-28. The structures of the obtained saponins were elucidated by a combination of mass spectrometry and 2D NMR spectroscopy. A study of acute toxicity, hemolytic, anti-inflammatory, immunoadjuvant and antifungal activity was carried out. Both saponins 1 and 2 were shown to exhibit immunoadjuvant properties within the vaccine composition with keyhole limpet hemocyanin-based immunogen. The availability of saponins 1 and 2 as individual pure compounds from the extract of the roots of A. gypsophiloides makes it a prospective source of immunoactive agents.
  Beilstein Journal of Organic Chemistry 01/2012; 8:763-75. · 2.52 Impact Factor
• Article: Effect of a carbon source on polyphosphate accumulation in Saccharomyces cerevisiae.

  Vladimir M Vagabov, Ludmila V Trilisenko, Tatiana V Kulakovskaya, Igor S Kulaev
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  ABSTRACT: The cells of Saccharomyces cerevisiae accumulate inorganic polyphosphate (polyP) when reinoculated on a phosphate-containing medium after phosphorus starvation. Total polyP accumulation was similar at cultivation on both glucose and ethanol. Five separate fractions of polyP: acid-soluble fraction polyP1, salt-soluble fraction polyP2, weakly alkali-soluble fraction polyP3, alkali-soluble fraction polyP4, and polyP5, have been obtained from the cells grown on glucose and ethanol under phosphate overplus. The dynamics of polyP fractions depend on a carbon source. The accumulation rates for fractions polyP2 and polyP4 were independent of the carbon source. The accumulation rates of polyP1 and polyP3 were higher on glucose, while fraction polyP5 accumulated faster on ethanol. As to the maximal polyP levels, they were independent of the carbon source for fractions polyP2, polyP3, and polyP4. The maximal level of fraction polyP1 was higher on glucose than on ethanol, but the level of fraction polyP5 was higher on ethanol. It was assumed that accumulation of separate polyP fractions has a metabolic interrelation with different energy-providing pathways. The polyphosphate nature of fraction polyP5 was demonstrated for the first time by (31)P nuclear magnetic resonance spectroscopy, enzymatic assay, and electrophoresis.
  FEMS Yeast Research 09/2008; 8(6):877-82. · 2.40 Impact Factor
• Article: Ustilagic acid secretion by Pseudozyma fusiformata strains.

  Tatiana V Kulakovskaya, Alexander S Shashkov, Ekaterina V Kulakovskaya, Wladyslav I Golubev
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  ABSTRACT: Eight strains of Pseudozyma fusiformata were examined for antifungal activity. All of them had the same spectrum of action and were active against many species of yeasts, yeast-like and filamentous fungi. They secreted glycolipids, which were purified from the culture liquid by column and thin-layer chromatography. According to nuclear magnetic resonance and mass-spectroscopy experiments all strains produced ustilagic acid, a cellobioside-containing 2,15,16-trihydroxypalmitic acid as aglycon, 3-hydroxycaproic acid and acetic acid as O-acylic substituents.
  FEMS Yeast Research 08/2005; 5(10):919-23. · 2.40 Impact Factor
• Article: Characterization of an antifungal glycolipid secreted by the yeast Sympodiomycopsis paphiopedili.

  Tatiana V Kulakovskaya, Alexander S Shashkov, Ekaterina V Kulakovskaya, Wladyslav I Golubev
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  ABSTRACT: An antifungal glycolipid was purified from the culture liquid of the ustilaginomycetous yeast Sympodiomycopsis paphiopedili by column and thin-layer chromatography. According to nuclear magnetic resonance and mass-spectroscopy experiments it was a cellobioside containing 2,15,16-trihydroxypalmitic acid as an aglycon. The minimal effective concentrations leading to ATP leakage and growth inhibition were 45 and 160 microg ml(-1) for Cryptococcus terreus and Candida albicans, respectively.
  FEMS Yeast Research 01/2005; 5(3):247-52. · 2.40 Impact Factor
• Article: ATP leakage from yeast cells treated by extracellular glycolipids of Pseudozyma fusiformata.

  Tatiana V Kulakovskaya, Ekaterina V Kulakovskaya, Wladyslav I Golubev
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  ABSTRACT: The ustilaginaceous yeast Pseudozyma fusiformata secreted glycolipids which were lethal to many yeasts and fungi more active at pH of about 4.0, and in the temperature range of 20-30 degrees C. Purified glycolipids enhanced non-specific permeability of the cytoplasmic membrane in sensitive cells, which resulted in ATP leakage and susceptibility of the cells to staining with bromocresol purple. Cells of Saccharomyces cerevisiae lost the ability to acidify the medium. Basidiomycetous yeasts were more sensitive to the glycolipids than ascomycetous ones. The minimal effective glycolipid concentration was 0.13 and 0.26 mg ml(-1) for Cryptococcus terreus and Filobasidiella neoformans, while for Candida albicans and Saccharomyces cerevisiae it was 1.0 and 1.6 mg ml(-1).
  FEMS Yeast Research 07/2003; 3(4):401-4. · 2.40 Impact Factor
• Article: The mycocidal, membrane-active complex of Cryptococcus humicola is a new type of cellobiose lipid with detergent features.

  Evgeny O Puchkov, Ulrich Zähringer, Buko Lindner, Tatiana V Kulakovskaya, Ulrich Seydel, Andre Wiese
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  ABSTRACT: The chemical composition of the mycocidal complex (formerly known as microcin) secreted by Cryptococcus humicola was investigated by chemical, mass spectrometric and nuclear magnetic resonance methods. The results indicate that the mycocidal complex is composed of glycolipids with a highly acetylated (up to five acetyl groups) cellobiose backbone [beta-D-Glcp-(1'-->4)-beta-D-Glcp] linked to the omega-hydroxyl group of alpha,omega-dihydroxy palmitate [16:0-alpha,omega-di-OH] with an unsubstituted carboxyl group. The acyl chain forming aglycon can be replaced by [18:0-(alpha,omega-di-OH)], [18:0-(alpha,omega-1,omega-tri-OH)], and [18:0-(alpha,omega-2,omega-tri-OH)]. The complex has a comparatively high surface activity; 0.5 mg/ml of it reduced the surface tension of 0.1 M NaHCO(3) from 71 mN/m to 37 mN/m and interfacial tension against n-hexadecane from 39 mN/m to 10 mN/m. The critical micelle concentration of the complex at pH 4.0, determined by the fluorometric method with N-phenyl-1-naphthylamine as fluorescent probe and by the De Nouy ring method, was 2 x 10(-5) M (taking the average molecular mass of the complex to be 750); it did not depend on the presence of 100 mM KCl and was an order of magnitude higher at pH 7.0. By fluorescence resonance energy transfer spectroscopy with N-(7-nitro-2,1,3-benzoxadiazol-4-yl)-phosphatidylethanolamine as energy donor and N-(rhodamine B sulfonyl)-phosphatidylethanolamine as energy acceptor the complex was shown to intercalate into the liposomal lipid matrix. Primary lesions caused by the complex in planar lipid bilayers were revealed as short-living current fluctuations of a broad spectrum of amplitudes. The mycocidal effect of the complex is suggested to be associated with its detergent-like properties.
  Biochimica et Biophysica Acta 02/2002; 1558(2):161-70. · 4.66 Impact Factor