Susanne Hausselt

Max Planck Institute for Medical Research, Heidelburg, Baden-Württemberg, Germany

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Publications (9)17.67 Total impact

  • T. Euler · S.E. Hausselt
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    ABSTRACT: How direction of image motion is detected as early as at the level of the vertebrate eye has been intensively studied in retina research. Although the first direction-selective (DS) retinal ganglion cells were already described in the 1960s and have since then been in the focus of many studies, scientists are still puzzled by the intricacy of the neuronal circuits and computational mechanisms underlying retinal direction selectivity. The fact that the retina can be easily isolated and studied in a Petri dish—by presenting light stimuli while recording from the various cell types in the retinal circuits—in combination with the extensive anatomical, molecular and physiological knowledge about this part of the brain presents a unique opportunity for studying this intriguing visual circuit in detail. This article provides a brief overview of the history of research on retinal direction selectivity, but then focuses on the past decade and the progress achieved, in particular driven by methodological advances in optical recording techniques, molecular genetics approaches and large-scale ultrastructural reconstructions. As it turns out, retinal direction selectivity is a complex, multi-tiered computation, involving dendrite-intrinsic mechanisms as well as several types of network interactions on the basis of highly selective, likely genetically predetermined synaptic connectivity. Moreover, DS ganglion cell types appear to be more diverse than previously thought, differing not only in their preferred direction and response polarity, but also in physiology, DS mechanism, dendritic morphology and, importantly, the target area of their projections in the brain.
    10/2012; 3(3):49-58. DOI:10.1007/s13295-012-0033-x
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    ABSTRACT: Dendritic signals play an essential role in processing visual information in the retina. To study them in neurites too small for electrical recording, we developed an instrument that combines a multi−photon (MP) microscope with a through−the−objective high−resolution visual stimulator. An upright microscope was designed that uses the objective lens for both MP imaging and delivery of visual stimuli to functionally intact retinal explants or eyecup preparations. The stimulator consists of a miniature liquid−crystal−on−silicon display coupled into the optical path of an infrared−excitation laser−scanning microscope. A pair of custom−made dichroic filters allows light from the excitation laser and three spectral bands (‘colors') from the stimulator to reach the retina, leaving two intermediate bands for fluorescence imaging. Special optics allow displacement of the stimulator focus relative to the imaging focus. Spatially resolved changes in calcium−indicator fluorescence in response to visual stimuli were recorded in dendrites of different types of mammalian retinal neurons.
    Pflügers Archiv - European Journal of Physiology 04/2009; 457(6). DOI:10.1007/s00424-008-0603-5 · 3.07 Impact Factor
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    ABSTRACT: DefinitionSomething that exists but is not spatiotemporally located, e.g. universals (whiteness, horseness), numbers or states of affairs.Possible WorldProperty
    Encyclopedia of Neuroscience, 01/2009: pages 3357-3555; , ISBN: 978-3-540-23735-8
  • Thomas Euler · Susanne Hausselt
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    ABSTRACT: It is essential for the visual system to detect image motion and to compute its direction and speed. Information on local motion is needed to predict the trajectory of moving objects, whereas information on global motion provides important feedback about body and head movement relative to the environment. That the computation of image motion starts in the retina was discovered more than 40 years ago: when Barlow and his colleagues recorded from retinal ganglion cells in the rabbit, they found a subset of cells that fired vigorously when an object moved in a certain (preferred) direction across their receptive fields but remained silent when the object moved in the opposite (null) direction. This chapter provides an overview of these direction−selective ganglion cells and the neuronal circuitry that underlies direction selectivity in the vertebrate retina.
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    Susanne E Hausselt · Thomas Euler · Peter B Detwiler · Winfried Denk
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    ABSTRACT: Author Summary The visual system dedicates substantial resources to detecting motion and its direction. For more than 40 years, researchers have tried to decipher the underlying computational mechanisms by which retinal neurons compute directed motion. One type of retinal interneuron involved in direction discrimination is the “starburst” amacrine cell. Starburst-cell dendrites are strongly activated by visual motion from their somata towards the dendritic tips, but not by motion in the opposite direction. It has been proposed, for example, that directional selectivity arises from lateral inhibitory interactions in which activated cells inhibit their neighbors. However, despite extensive modeling, the underlying physiological mechanism has remained elusive. Here, by combining whole-cell recordings, two-photon microscopy, and modeling, we show that discrimination of motion direction in starburst-cell dendrites does not require lateral inhibitory interactions in the retina, but can be generated by a “dendrite-autonomous” computation, which relies on intrinsic electrical mechanisms. Blocking inhibitory interactions does not eliminate directional responses, whereas differential activation of voltage-gated membrane conductances and a dendritic voltage gradient can provide the necessary spatial asymmetry to produce directional signals. The computation underlying dendrite-autonomous direction selectivity may represent one of the most intricate examples to date of dendritic information processing.
    PLoS Biology 08/2007; 5(7):e185. DOI:10.1371/journal.pbio.0050185 · 11.77 Impact Factor
  • Susanne Hausselt · Thomas Euler · P. B. Detwiler · Winfried Denk
  • Susanne Hausselt
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    ABSTRACT: In goldfish, the retinal pathways involved in motion coding have been demonstrated to have an L-cone dominated action spectrum (S. Schaerer, C. Neumeyer, Motion detection in goldfish investigated with the optomotor response is "color blind", Vision Res. 36 (1996) 4025-4034). The neurotransmitters involved in retinal motion coding mechanisms, and the relevance of these retinal motion coding mechanisms for motion perception, are little investigated in fish. In this study, the optomotor response was used to investigate the effect of antagonists on different receptor types for acetylcholine (ACh), GABA, for the dopamine D2-receptor (D2-R) - which is known to modulate the action spectrum in motion coding (C. Mora-Ferrer, K. Behrend, Dopaminergic modulation of photopic temporal transfer properties in goldfish retina investigated with the ERG, Vision Res. 44 (2004) 2067-2081) - and of an agonist for against the mGluR6-receptor (mGluR6) on goldfish motion vision in the photopic range. Blockade of nicotinic ACh-R, GABAa-R and both GABAa- and GABAc-R eliminated the optomotor response completely. Neither a muscarinic ACH-R antagonist, a D2-R antagonist or a mGluR6-agonist affected goldfish motion vision. The pharmacological profile of the goldfish optomotor response resembles the pharmacological profile of direction-selective ganglion cells (DS-GC) described for vertebrate retinas in electrophysiological experiments, e.g. (S. Weng, W. Sun, S. He, Identification of ON-OFF direction-selective ganglion cells in the mouse retina, J. Physiol. 562 (2005) 915-923). This indicates that cells with direction-selective receptive field properties exist in the goldfish retina. It is proposed that these cells provide the input for the full field motion perception in goldfish.
    Brain research 11/2005; 1058(1-2):17-29. DOI:10.1016/j.brainres.2005.07.073 · 2.83 Impact Factor
  • Thomas Euler · Susanne Hausselt · Peter D. Detwiler · Winfried Denk

Publication Stats

133 Citations
17.67 Total Impact Points

Institutions

  • 2007–2009
    • Max Planck Institute for Medical Research
      • Department of Biomedical Optics
      Heidelburg, Baden-Württemberg, Germany
  • 2005
    • Johannes Gutenberg-Universität Mainz
      • Institute of Zoology
      Mayence, Rheinland-Pfalz, Germany