S Tassiopoulos

Laiko Hospital, Athínai, Attica, Greece

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Publications (13)22.1 Total impact

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    ABSTRACT: In patients with Systemic lupus erythematosus (SLE), Raynaud phenomenon (RP) is frequently present and associated with pulmonary hypertension (PHT). Elevated pulmonary artery systolic pressure (PASP) is an indicator of PHT and can be estimated noninvasively. We attempt to explore the significance of RP in SLE and to correlate it with clinical and serological parameters of the disease. The study population consisted of 34 patients (age, sex and disease duration matched) who fulfilled the revised SLE criteria of the American College of Rheumatology, and were categorized into two groups: Group 1 had patients having SLE and RP (2 males/15 females, mean age 45 +/- 18 years) and group 2 had patients with SLE but without RP (3 males/14 females, mean age 40 +/- 14 years. Detailed cardiac ultrasound was performed including measurement of PASP, while clinical and serological features of both groups were collected and correlated. Significant differences were shown in the presence of arterial hypertension (P < 0.05), arthralgias (P < 0.005), arthritis (P < 0.05), myalgias (P < 0.05), alopecia (P < 0.05) and PASP (P < 0.0001). No difference was observed among the cardiac ultrasound indices and the ejection fraction between the two groups. PASP was significantly correlated with RP, while no correlation was observed regarding the disease duration. In patients with SLE, the presence of RP was associated with elevation in PASP. Further investigation is needed to clarify the significance of this relation.
    Lupus 01/2007; 16(7):505-8. · 2.78 Impact Factor
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    ABSTRACT: Without Abstract
    Annals of Hematology 09/2006; 85(8):555-6. · 2.87 Impact Factor
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    ABSTRACT: Long treatment with theophylline is often followed by a reduction of Hb levels, which is usually ascribed to the antagonistic activity between theophylline and adenosine (ADO) which leads to decreased erythropoietin (EPO) synthesis. This view is not unanimously accepted. To evaluate the influence of theophylline on erythropoiesis in chronic obstructive pulmonary disease (COPD), the hematologic parameters and EPO values were estimated in 32 patients with COPD. Twelve of them had been treated with theophylline for at least 1 year; the other 20 patients had never received this drug. 34 sex- and age-matched healthy volunteers served as controls. We further studied the development of BFU-E-derived colonies in semisolid methylcellulose cultures in blood samples from 7 patients randomly selected from both groups. In addition, we studied the effects of theophylline on the erythroid cell development by adding this agent in erythroid cell cultures from 6 healthy volunteers at various concentrations. No significant differences were found in the hemoglobin and serum erythropoietin values between the 2 COPD groups (treated and untreated). Unlike untreated patients and controls, the serum of the theophylline-treated patients produced a significant growth inhibition of erythroid bursts (p<0.05); the in vitro use of theophylline showed a concentration dependent inhibition (p<0.001). The fact that the erythropoietin amount was consistently more than adequate for optimal erythropoietic development in culture dishes, suggests a direct inhibitory action of theophylline on erythropoiesis, possibly through the enhanced apoptosis induced by this drug in several other types of cells. The fact that the Hb levels did not show any significant differences implies the involvement of other factors both on the serum levels of the drug and on the erythropoietic response in each individual marrow.
    Haema 6, 522-528, 2003. 01/2003; 6:522.
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    ABSTRACT: This study was designed in order to resolve the conflicting information related to macrocytosis in hypoxemic chronic obstructive pulmonary disease (COPD) and search for potential underlying mechanisms. We evaluated the hematologic parameters, F cell percentage, blood gases and serum erythropoietin (Epo) levels in 32 COPD clinically stable patients and 34 sex- and age-matched non-smoker healthy volunteers. An increased MCV was observed in almost half of the hypoxemic COPD cases (43.75%), while erythrocytosis developed to a lesser degree (37.5%). The erythropoietic response did not correlate with the severity of hypoxia. Moreover, no significant correlation was found between macrocytosis and hypoxemia or erythrocytosis and red cell size. In some cases the two phenomena occurred independently. The F cell percentage was significantly above normal in the group of COPD patients (p<0.01) and displayed a strong correlation with the respective MCV values (n=32, r=0.41, p<0.05). So, it is assumed that the acute erythropoietic stress occurring repeatedly in these patients as a result of their frequent episodes of hypoxemia may lead to waves of release of relatively immature large red cells from the marrow, including an increased number of F-cells, reflecting the recruitment of normally dormant BFU-E, which maintain the program for γ-chain synthesis. The fact that erythrocytosis and macrocytosis, both being triggered by hypoxemia, do not occur consistently in all COPD patients indicates that many other factors may also intervene.
    Haema 6, 366-371, 2003. 01/2003; 6:366.
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    ABSTRACT: Idiopathic pulmonary fibrosis (IPF) is a chronic inflammatory process characterized by severe derangement of gas exchange in the advanced stages of disease. However, erythrocytosis is infrequent in IPF. The aim of this study was to investigate the potential relation between the blunted erythropoietic response and the chronic inflammation. 9 patients (6 males and 3 females) suffering from IPF with profound hypoxemia (PO2 < 65mmHg) and 34 sex- and age-matched healthy volunteers participated in the study. We evaluated the hematologic parameters, serum EPO, TNF-α, IL-6 and IL-8 levels. We also studied the development of BFU-E-derived colonies in semisolid methylcellulose cultures in blood samples from all patients.RESULTS: Hb and serum EPO levels were almost comparable between the 2 studied groups. On the contrary, serum TNF-α, IL-6, IL-8 values were significantly higher in patients with IPF (p<0.05, p<0.01, p<0.001 respectively). IPF sera induced a significant growth inhibition of erythroid bursts arising from mononuclear cells of either patients or controls compared with AB heat inactivated serum (p<0.05 and p<0.01 respectively). Moreover, there was an apparent increment in the number of BFU-E colonies when patients’ mononuclear cells were cultured in comparison with those of healthy subjects (p<0.05). Our findings suggest that in IPF there is an increased number of primitive erythroid progenitors, which fail to proliferate and differentiate in vivo, suggesting a kind of ineffective erythropoiesis. As a consequence, Hb levels do not rise in proportion to the severity of hypoxemia. Cytokines released from alveolar macrophages seem to have not only local but also systemic effects, since the serum of these patients directly suppressed erythropoiesis. However, the suboptimal erythropoietic response to hypoxia can not be entirely attributed to this suppression; here it is possible that several other factors interfere, synergistically or additively acting.
    The Hematology Journal 01/2003; 4:52. · 1.86 Impact Factor
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    ABSTRACT: Macrocytosis is a common finding in patients with chronic obstructive pulmonary disease (COPD ). The cause for the elevation of mean corpuscular volume (MCV) in these patients remains elusive. The aim was to determine the extent of macrocytosis in COPD patients and to search for possible causative factors. METHODS: We evaluated the hematologic parameters, F cell percentage, blood gases and serum erythropoietin (Epo) levels in 32 COPD clinically stable patients and 34 sex- and age-matched non-smoker healthy volunteers. An increased MCV was observed in almost half of the hypoxemic COPD cases (14/32 or 43.75%), while erythrocytosis developed to a lesser degree (37.5%). The erythropoietic response did not correlate with the severity of hypoxia. Moreover, no significant correlation was found between macrocytosis and hypoxemia or erythrocytosis and red cell size. In some cases the two phenomena occurred independently. The F cell percentage was significantly above normal in the group of COPD patients (p<0.01) and displayed a strong correlation with the respective MCV values (n=32, r=0.41, p<0.05). This finding is taken as an indication supporting the hypothesis we put forward to explain the macrocytosis often observed in COPD, i.e., that the acute erythropoietic stress occurring repeatedly in these patients as a result of their frequent episodes of hypoxemia may lead to waves of release of relatively immature red cells from the marrow, including an increased number of F-cells, reflecting the recruitment of normally dormant BFU-E, which maintain the program for *-chain synthesis. The fact that erythrocytosis and macrocytosis, both being triggered by hypoxemia, do not occur consistently in all COPD patients indicates that many other factors may also intervene. Thus, the macrocytosis often observed in COPD may be considered to reflect, at least partially, an acute erythropoietic stress leading to premature release of large erythrocytes from the marrow through increased Epo synthesis.
    The Hematology Journal 01/2003; 4:51. · 1.86 Impact Factor
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    ABSTRACT: The aim of this study was to assess the erythropoietic response to hypoxaemia in patients with diffuse idiopathic pulmonary fibrosis (DIPF), and to speculate on the underlying mechanisms. Patients on an established chronic respiratory failure due to DIPF or chronic obstructive pulmonary disease (COPD) were studied. The erythropoietic response to hypoxaemia in both conditions was assessed. We studied 18 patients with DIPF and 29 patients with COPD in respiratory failure in a stable stage, free from acute infection and congestive heart failure. Blood gases, erythrocytic parameters, as well the serum levels of iron, ferritin and erythropoietin were determined. All the DIPF patients studied, apart from two, had normal or subnormal haematocrit values. The patients with COPD had an inconsistant response to hypoxaemia; 12 had normal or subnormal haematocrit values and the remaining 17 were erythraemic. The mean value of erythropoietin (EPO) in both DIPF and COPD patients was significantly higher than normal. In conclusion, patients with DIPF exhibit a lack of erythropoietic response to hypoxaemia, despite the augmented erythropoietin levels. This may reflect a defective bone marrow erythropoietic response in DIPF patients. It is suggested that the pathophysiology of DIPF underlies this mechanism.
    Respiratory Medicine 07/2001; 95(6):471-5. · 2.59 Impact Factor
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    ABSTRACT: A case of pyomyositis is presented. This case is unique in the literature as at least 29 abscesses were detected, affecting the vast majority of big muscle groups. We outline the origin of this disease entity which selectively affects striated muscles. We also discuss its natural history and management strategy.
    Scandinavian Journal of Infectious Diseases 02/2001; 33(11):857-8. · 1.71 Impact Factor
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    ABSTRACT: We measured the 2,3-diphosphoglycerate inraerythrocytic concentration in 24 normal controls and in 24 hypertensives before and following drug therapy. In hypertensives the 2,3-diphosphoglycerate concentration was higher than that of the controls (14.96 mumol/g Hb vs 13.26 mumol/g Hb respectively); the difference is statistically significant (p < 0.001). Following control of the hypertension by drug therapy, the 2,3 DPG levels in the patients studied do not seem to differ statistically from those of the controls. This may be a consequence of lower cardiac output in hypertension which results to a lower tissue perfusion, leading to an increased concentration of deoxygenated haemoglobin in the vein blood. Measurement of 2,3-diphosphoglucerate may prove of value in estimating tissue perfusion in hypertension.
    Panminerva medica 09/1997; 39(3):186-8. · 2.28 Impact Factor
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    ABSTRACT: Erythropoietin levels were determined in 50 Greek females: 20 beta-thalassaemia (beta-thal) heterozygotes, 15 with a diagnosis of iron-deficiency anaemia and 15 normal controls. In beta-thal trait carriers, the erythropoietin levels were slightly higher than in normal controls (16.65 +/- 4.43 vs. 12.84 +/- 2.47 mU/ml); these levels were significantly lower than those in iron-deficient subjects with the same degree of anaemia (55.24 +/- 31.35 mU/ml). In both groups, the erythropoietin levels are statistically correlated with the severity of anaemia (r = -0.537 p < 0.05 for iron deficiency; r = -0.610 p < 0.01 for beta-thal heterozygotes). In beta-thal heterozygotes, a close inverse correlation with red cell number and erythropoietin levels was also noted. It is suggested that microcytosis accompanying beta-thal trait constitutes an additional factor intervening in the regulation of erythropoiesis.
    Acta Haematologica 01/1997; 98(3):147-9. · 0.89 Impact Factor
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    ABSTRACT: In 45 beta-thalassaemia heterozygotes and in 38 normal controls we determined by ultrasound tomography: (a) the surface of the projection of the spleen to thoracic and abdominal wall, (b) the maximum diameters of the organ, and (c) its volume. The volume of the spleen is significantly bigger in heterozygotes as opposed to normals (132.94 +/- 41.76 and 80.29 +/- 25.88, respectively). In 17.8% of heterozygotes a palpable spleen was found. The findings of this study lead to the hypothesis that in all heterozygotes the final volume of the organ is increased; however, in only 17.8% of them a palpable spleen is found.
    Haematologia 02/1995; 26(4):205-9.
  • Clinical and experimental rheumatology 23(1):122-3. · 2.66 Impact Factor
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    ABSTRACT: Adamantiades-Behçet's disease is a multisystem recurrent syndrome with vasculitis being the underlying histopathological lesion. We report on a patient with Behçet's disease who developed a renal mass raising the suspicion of a malignant neoplasm. The pathologic examination revealed an inflammatory pseudotumor. The concurrent presentation of these two entities seems to be more than coincidental.
    American Journal of Nephrology 20(3):217-21. · 2.62 Impact Factor