S K Raghuwanshi

Publications (4)9.91 Total impact

• Article: Immunolocalization of secretory proteins of Aspergillus fumigatus using monospecific polyclonal antibodies in a murine model.

  S K Raghuwanshi, M Kumar, A Kavishwar, A K Chaturvedi, P S R Murthy, P K Shukla
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  ABSTRACT: The incidence of life-threatening mycoses caused by opportunistic fungi has increased dramatically in recent years with members of the genera Candida and Aspergillus being the most commonly encountered species. Prompt initiation of antifungal therapy for good prognosis of such cases is highly dependent on accurate diagnosis. The potential of metabolic antigens in the diagnosis of aspergillosis was investigated in the present study. Two proteins of 18 and 70 kDa were identified with success rate of 35% and 60% respectively based on their reactivity with patient sera of clinically diagnosed cases of aspergillosis. The antibodies raised against 70 and 18 kDa proteins in rabbits were found to be useful in detection of A. fumigatus in the kidneys of a mouse model of aspergillosis.
  Mycoses 10/2005; 48(5):313-20. · 2.25 Impact Factor
• Article: Identification of novel antifungal nonapeptides through the screening of combinatorial peptide libraries based on a hexapeptide motif.

  B Kundu, T Srinivasan, A P Kesarwani, A Kavishwar, S K Raghuwanshi, S Batra, P K Shukla
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  ABSTRACT: Four sets of mixture based nonapeptide libraries derived from an antifungal hexapeptide pharmacophore Arg-D-Trp-D-Phe-Ile-D-Phe-His-NH(2) (II) have been synthesized. The three C-terminal positions 7, 8 and 9 were subject to randomization using 19 genetically coded amino acids. They were then screened for their antifungal activity against Candida albicans and Cryptococcus neoformans in order to quantify inhibition at each step of the nonapeptide sublibrary deconvolution. The studies led to the identification of several novel nonapeptides with potent antifungal activity. Two of the nonapeptides exhibited approximately 17-fold increase in the activity in comparison to the lead hexapeptide motif His-D-Trp-D-Phe-Phe-D-Phe-Lys-NH(2) (I) against C. albicans.
  Bioorganic & Medicinal Chemistry Letters 07/2002; 12(11):1473-6. · 2.55 Impact Factor
• Article: Combinatorial approach to lead optimization of a novel hexapeptide with antifungal activity.

  B Kundu, S K Rastogi, S Batra, S K Raghuwanshi, P K Shukla
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  ABSTRACT: Three sets of sublibraries of an antifungal lead peptide His-D-Trp-D-Phe-Phe-D-Phe-Lys-NH2 (I) have been prepared by introducing variations at positions 1, 4 and 6. They were screened for their antifungal activity against C. albicans and C. neoformans in order to quantify inhibition at each step of the hexapeptide sublibrary iteration. The studies led to the identification of Arg-D-Trp-D-Phe-Ile-D-Phe-His-NH2 as a novel hexapeptide with potent antifungal activity against both C. albicans and C. neoformans.
  Bioorganic & Medicinal Chemistry Letters 09/2000; 10(16):1779-81. · 2.55 Impact Factor
• Article: Suitably functionalised pyrimidines as potential antimycotic agents.

  N Agarwal, S K Raghuwanshi, D N Upadhyay, P K Shukla, V J Ram
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  ABSTRACT: Various suitably functionalized pyrimidine derivatives have been synthesized to explore their potential as antimycotic agents. Some of the synthesized compounds 4c, 4d, 8a-e have shown highly significant in vitro antifungal activity against five human pathogenic fungi.
  Bioorganic & Medicinal Chemistry Letters 05/2000; 10(8):703-6. · 2.55 Impact Factor