S K Matelo

Publications (3)0.27 Total impact

• Article: Bacterial markers of periodontal diseases and their practical significance in dentistry.

  A B Chukhlovin, A M Solovyova, S K Matelo, I V Kobiyasova, E B Morosova, A V Hokhlacheva, B G Teplyakov, K A Syssoev, V E Konstantinova, L N Matelo, Areg A Totolian
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  ABSTRACT: The incidence and prevalence of Actinobacillus actinomycetemcomitans, Porphyronmonas gingivalis, and Tannerella forsythensis in specimens of subgingival dental deposit were evaluated in 495 residents of St. Petersburg aged 6-82 years. The microorganisms were detected by gene-specific PCR of 16S rDNA. In accordance with age-specific increase in the incidence of gingival diseases, the percentage of samples containing T. forsythensis and P. gingivalis was significantly higher in adult and elderly patients in comparison with adolescents. The presence of T. forsythensis significantly correlated with the presence of gingivitis and dental deposit. In addition, the incidence of T. forsythensis was significantly higher in tobacco smokers. These results attest to a relationship between T. forsythensis infection and more frequent periodontal diseases associated with aging and tobacco smoking.
  Bulletin of Experimental Biology and Medicine 10/2007; 144(4):546-50. · 0.27 Impact Factor
• Article: [PCR detection of periodontal bacteria and Streptococcus mutans in the biological samples from the oral cavity].

  A B Chukhlovin, A M Solov'eva, S K Matelo, A V Dmitriev, E B Morozova, A P Bobrov, A A Totolian
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  ABSTRACT: The paper describes an optimized technique for DNA isolation from the Streptococcus mutans cultures and from the clinical samples including proteinase K treatment and detergent lysis, followed by sorbent-based enrichment. This technique was employed for isolating DNA from the periodontal bacteria Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, and Bacteroides forsythus. A multiplex PCR technique was adapted for large-scale studies of clinical samples. An original PCR method was developed for the semiquantitative detection of Str. mutans, showing the sensitivity of 100 genome copies per reaction. Extensive studies of approximately 2,000 individuals have demonstrated that these methods are applicable to the study of the dental and gingival microflora in the biological sample from the oral cavity of persons with dental diseases from various age groups.
  Klinicheskaia laboratornaia diagnostika 05/2007;
• Article: [Epidemiological study of the prevalence periodontal pathogeneus microflorae of oral cavity in the population of Russia].

  A M Solov'eva, S K Matelo, A A Totolian, A B Chukhlovin, V E Konstantinova, I V Kobiiasova, L N Matelo, T V Smirnova, N A Shamanova, L A Gagaleva, S N Nosov, G I Kuz'minykh, T N Stati, A N Malygina, V P Ulitina, K A Sysoev, A V Dmitriev, E B Morozova, A V Khokhlacheva, B G Tepliakov
  Stomatologiia 02/2005; 84(5):14-20.