S Di Sario

Publications (2)3.95 Total impact

• Article: Cellular uptake and delivery monitoring of liposome/DNA complexes during in vitro transfection of CFTR gene.

  A L Serafino, G Novelli, S Di Sario, A Colosimo, P Amicucci, F Sangiuolo, G Mossa, B Dallapiccola
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  ABSTRACT: The cellular uptake and distribution of cationic liposomes Dc-Chol/DOPECFTR gene complexes were assessed by electronic and confocal laser scanner microscopy (CLSM) for the CFTR gene transfer to human adenocarcinoma and tracheal epithelial cell lines. Cationic lipid forms unilamellar and multilamellar vesicles capable of rapid and efficient transport of gene into target cells. The number of fluorescent complexes was increasing with time in cells up to 6 hours showing a punctate and homogeneous DNA distribution in the cytoplasmatic and nuclear compartments, including the nucleolus. No significant difference in the biochemical and cellular behavior was observed between the investigated system and other systems previously tested. This study adds new insights into the CFTR cationic liposome-mediated gene delivery.
  Biochemistry and molecular biology international 03/1999; 47(2):337-44.
• Article: Surfactant as modulating agent of enzyme-loaded liposome activity.

  M C Annesini, C M Braguglia, A Memoli, L G Palermiti, S Di Sario
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  ABSTRACT: Large phosphatydilcholine unilamellar vesicles appear to be suitable controlled and protective delivery systems of beta-galactosidase. Kinetic measurements carried out on intact loaded liposomes show that most of the enzyme is entrapped inside the liposomes and its activity is latent. Nevertheless, intact liposomes also show significant activity, which can be controlled by addition of detergent. At sublytic detergent concentrations, liposome enzymatic activity reaches values two or three times greater than those of intact liposomes. This increase seems to be due to membrane structure modification that also enhances the substrate permeability across the bilayer. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 261-266, 1997.
  Biotechnology and Bioengineering 07/1997; 55(2):261-6. · 3.95 Impact Factor