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ABSTRACT: We report the application of an integrated optical Young interferometer sensor for ultrasensitive, real-time, direct detection of viruses. We have validated the sensor by detecting herpes simplex virus type 1 (HSV-1), but the principle is generally applicable. Detection of HSV-1 virus particles was performed by applying the virus sample onto a sensor surface coated with a specific antibody against HSV-1. The performance of the sensor was tested by monitoring virus samples at clinically relevant concentrations. We show that the Young interferometer sensor can specifically and sensitively detect HSV-1 at very low concentrations (850 particles/mL). We have further demonstrated that the sensor can specifically detect HSV-1 suspended in serum. Extrapolation of the results indicates that the sensitivity of the sensor approaches the detection of a single virus particle binding, yielding a sensor of unprecedented sensitivity with wide applications for viral diagnostics.
Nano Letters 03/2007; 7(2):394-7. · 13.20 Impact Factor
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ABSTRACT: We demonstrate that in a sensor based on a multichannel Young interferometer, the phase information obtained for different pairs of channels can be used to correct the long-term instability (drift) due to temperature differences between measuring and reference channels, the drift in the alignment of the setup, etc. Experiments show that the nature of a major part of the drift is such that the drift present in one of the channels can be determined by interpolation of the drift measured in the two adjacent channels. It is shown that a drift reduction of 10 times can be achieved as compared with the situation in which no correction is applied. We anticipate that these findings will permit the exploitation of the extreme sensitivity of interference-based sensors to a much greater extent.
Applied Optics 07/2005; 44(17):3409-12. · 1.41 Impact Factor
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ABSTRACT: We report on the design, realization, and characterization of a four-channel integrated optical Young interferometer device that enables simultaneous and independent monitoring of three binding processes. The generated interference pattern is recorded by a CCD camera and analyzed with a fast-Fourier-transform algorithm. We present a thorough theoretical analysis of such a device. The realized device is tested by monitoring glucose solutions that induce well defined phase changes between output channels. The simultaneous measurement of three different glucose concentrations shows the multipurpose feature of such devices. The observed errors, caused by the mismatching of spatial frequencies of individual interference patterns with those determined from the CCD camera, are reduced with different reduction schemes. The phase resolution for different pairs of channels was approximately 1 x 10(-4) fringes, which corresponds to a refractive-index resolution of approximately 8.5 x 10(-8). The measured sensitivity coefficient of the phase change versus refractive-index change of approximately 1.22 x 10(3) x 2pi agrees well with the calculated coefficient of approximately 1.20 x 10(3) x 2pi.
Applied Optics 11/2003; 42(28):5649-60. · 1.41 Impact Factor
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ABSTRACT: In this paper, we report the realization and characterization of a four-channel integrated uplical Young interferometer (YI), which enables simultaneous and independent monitoring of three binding processes. The simultaneous and independent measurement of three different glucose concentrations shows the multi-purpose feature of such device.' The phase resolution for different pairs of channels was -1 ~ 1 0 ~ fringes, which corresponds to a rehctive index resolution of -8.5~10-~. The observed errors, which are caused due to mismatching of spatial frequencies of individual interference panerns with those determined from the CCD camera, have been reduced by using different reduction schemes. In addition, we have investigated a novel method for discrimination of the refractive index change from the thickness of a bound layer during an immunoreaction, as well as measuring the temperature change that takes place during such a process.
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ABSTRACT: The use of an optical-fibre polarimeter as a chemical sensor is demonstrated. The compound to be detected is allowed to adsorb onto a decladded 5 cm length of the fibre. The fibre is polarization maintaining with an elliptical fibre core and a D-shaped geometry. The overall retardation stability of this fibre polarimeter is ≈ 0.5 × 2π rad m−1 K−1. With this sensor adsorption processes of proteins can be followed on-line. The resulting relative phase retardations caused by the growth of a monolayer of antibodies (αhCG, αhSA) are ≅ 0.25 × 2π. For the much smaller protein hSA, this value is ≅ 0.1 × 2π.
Sensors and Actuators B: Chemical.
