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Publications (10)7.23 Total impact

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    ABSTRACT: This report describes the application of ten bioassays (battery of tests approach) to waters and sediments collected from the tar sands area of northern Alberta, Canada. In this study there were three main goals: (1) to establish the presence and sources of toxicants in these northern waters and sediments, (2) to determine if their presence is related to sediment composition, and (3) to establish which of the various sediment extracting procedures used was most efficient in producing responses in the bioassays used.Results of these investigations indicated that there were two obvious sources of toxicants/genotoxicants, the tar sands extracting area and streams passing through tar sands or oil shales and at least two unknown sources. Based on the techniques used in this study, there does not appear to be a strong sediment structure–toxicant response relationship. Extraction procedure results were variable and are discussed in detail in the report. © by John Wiley & Sons, Inc.
    Environmental Toxicology and Water Quality 05/2006; 10(2):107 - 117.
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    ABSTRACT: With many environmental water samples, excluding industrial and domestic effluents, it has been found necessary to concentrate the water samples prior to testing with various bioassays for toxicant/genotoxicant content. In this report we describe four water concentration procedures: (1) flash evaporation, (2) Blue Rayon, (3) Empore disk, and (4) membrane filtration. For the water samples tested in this study a flash evaporation procedure and the membrane filtration procedure were the most promising sample concentration procedures. Results are discussed and future research directions are proposed. © 1993 John Wiley & Sons, Inc.
    Environmental Toxicology and Water Quality 05/2006; 8(4):397 - 407.
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    ABSTRACT: The distribution of female hormones, 17beta-estradiol and estrone, was determined in effluents of 18 selected municipal treatment plants across Canada. Replicate 24-h composite samples were collected from the influent and final effluent of each treatment plant, and the removal efficiency compared to the operational characteristics of the plants. In conventional activated sludge and lagoon treatment systems, the mean concentrations of 17beta-estradiol and estrone in influent were 15.6 ng/l (range 2.4-26 ng/l) and 49 ng/l (19-78 ng/l). In final effluents, the mean concentrations of both 17beta-estradiol and estrone were reduced to 1.8 ng/l (0.2-14.7 ng/l) and 17 ng/l (1-96 ng/l), respectively. 17beta-estradiol was removed effectively, >75% and as high as 98%, in most of the conventional mechanical treatment systems with secondary treatment. The removal of estrone was much more complex with removal varying from 98% to situations where the concentrations in the effluent were elevated above that detected in the influent. The estrogenicity, measured using a transfected estrogen receptor in yeast (YES) assay, was also variable, ranging from high removal to elevations of estrogenicity in final effluent. Although the apparent removals were not statistically correlated with either hydraulic (HRT) or solid (SRT) retention times, plants or lagoons with high SRT were very effective at reducing the levels of hormones. Well-operated plants that achieved nitrification also tended to have higher removal of hormones than those that did not nitrify. Laboratory aerobic reactor experiments confirmed the rapid removal of 17beta-estradiol, estrone, and estrogenicity when exposed to sewage slurries.
    Science of The Total Environment 01/2005; 336(1-3):155-70. · 3.26 Impact Factor
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    ABSTRACT: Safe potable water is a luxury that is generally unavailable to the majority of rural and suburban populations of developing, underdeveloped, and often developed countries. Important consid- erations in the development and maintenance of safe water supplies is the availability and use of efficient, inexpensive, and appropriate technology for removing microbial hazards, parasites, and toxicants. The Manz intermittent slow sand filter was known to be user friendly, small enough to fit into the smallest kitchen, and could remove up to 97% of the fecal coliforms present in the raw water before treatment by the Manz filter. This filter was evaluated for its ability to remove parasitic cysts and toxicants as well as bacteria. Using two different filters and two different water supplies our results indicated that the intermittent slow sand filter could remove 83+% total heterotrophic bacterial populations, 100% of Giardia cysts, 99.98% of Cryptosporidium oocysts, and 50 -90% of organic and inorganic toxicants when administered in concentrations varying from 10 - )100 = environmental pollution levels. Methodology details are provided in the paper. Q 1999 by John Wiley & Sons, Inc. Environ Toxicol 14: 217)225, 1999
    Environmental Toxicology - ENVIRON TOXICOL. 01/1999; 14(2):217-225.
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    ABSTRACT: Five samples were collected from the Rapel River Basin near the city of Rancagua and three from the city of Temuco. Three of the samples were collected from raw drinking water supplies. The following bioassays were performed on some or all of the samples: Microtox; Microtox solid phase test; SOS-Chromotest with and without S9; Toxi-Chromotest; Sediment-Chromotest; Panagrellus redivivus percent survival and percent maturation; submitochondrial reverse electron transfer and forward electron transfer tests; Daphnia magna 24 h acute toxicity; ECHA biocide monitor, and the competitive immunoassay tests for benomyl, metolachlor, atrazine, and triazines. All the sampling sites were positive for the presence of genotoxicants requiring S9 activation while three sites also indicated the presence of direct-acting genotoxicants/mutagens (−S9). Also, all the sites were positive for the presence of pesticides. In some samples there was 100% inhibition of P. redivivus maturation. Details and discussion on the implication of the results are presented. © 1996 by John Wiley & Sons, Inc.
    Environmental Toxicology and Water Quality 12/1998; 11(3):237 - 247.
  • Environmental Toxicology and Water Quality 01/1998; 13(4):313-322.
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    ABSTRACT: A former mercury plant, where mercury salts and organomercurials for pesticide use were produced, caused soil contamination in high concentrations. Typical organomercurial products included ethylmercury, phenylmercury, methoxyethylmercury and ethoxyethylmercury compounds. Risk assessment of these sites must be carried out before any major clean-up processes can be planned. A sensitive speciation technique for the various organomercury species in environmental matrices is a prerequisite for toxicity investigations. In this connection, a high-performance liquid chromatography-atomic fluorescence spectrometry (HPLC-AFS) technique has been developed to differentiate between and determine the presence of eight organomercury compounds in environmental samples. Using this technique, methylmercury, ethylmercury and phenylmercury and some unknown organomercury species were found in soil samples collected from the sites of an old mercury products producing plant. With regard to risk assessment, it is necessary to assess the toxicity of the organomercurials. As different microbial metabolic pathways react differently to mercury and its compounds, batteries of bioassays are, therefore, useful to evaluate the toxicity of pollutants. To describe the toxicity and genotoxicity of MeHg+, MeOEtHg+, EtHg+, EtOEtHg+ and PhHg+, p-tolymercury chloride, nitromersol and Hg2+ six bioassays were used: resazurin reduction method, Spirillum volutans test, nematode toxicity assay Panagrellus redivivus, Toxi-Chromotest and SOS-Chromotest. A ranking of the toxicity of the organomercurial is shown. The SOS-Chromotest indicated genotoxicity for 5-7 organomercurials.
    The Analyst 04/1995; 120(3):721-4. · 3.97 Impact Factor
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    ABSTRACT: Over the past eight years we have been evaluating a variety of sediment extraction procedures and have finally settled for a three phase sequential procedure which involves pore water extraction followed by Milli‐Q water extraction. Then the dewatered sediment is extracted by a solution containing 10% methanol, 10% DMSO and 80% Milli‐Q water. This three phase sequential extraction procedure was applied to Hamilton Harbour sediments and the extracts were tested for toxicant activity by the battery of tests approach. Based on these samples, it would appear that pore water bioassay results are probably most indicative of the bioavailable toxicants load in Hamilton Harbour sediments.
    Journal of Environmental Science and Health Part A-toxic/hazardous Substances & Environmental Engineering - J ENVIRON SCI HEALTH A. 01/1994; 29(8):1649-1661.
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    ABSTRACT: In trying to establish the extent and degree of impact of point and nonpoint source contaminants on riverine systems, it is important to know the implications of the data obtained from various sampling points in a riverine system. Spatial variability between water and/or sediment samples collected in close approximation at the same sites was assessed by the battery of tests approach. In these samples there appeared to be no consistent relationship between sediment structure, microbial population, and toxicant concentrations. Furthermore the ATP-TOX System and Mutatox tests were the most responsive tests in all types of samples. Since sediments with their bound contaminants may be an important factor in this data interpretation, different methods for releasing and concentrating the sediment bound contaminants were evaluated. The results and implications of these results are described.
    Environmental Toxicology and Water Quality 07/1991; 6(3):309 - 327.
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    ABSTRACT: In this study, a new approach has been taken to evaluate Saint John River water and sediment conditions. A battery of biochemical, microbiological and bioassay tests were used to identify degraded or degrading sediments and waters. Data were obtained from waters and sediments at 38 sites within the Saint John River Basin. The data suggested that the following four sites had the highest priority concern: Little River No. 34, Grand Bay, Saint John River near Boars Head No. 33, Madawaska River below mill No. 7 and St Francois-de-Madawaska, mill stream No. 2. The data also indicated that microbial population, biochemical or bioassay tests performed independently do not provide realistic evaluations of priority concern areas and that the “battery of tests” approach is necessary to provide additional information.
    Water Research. 01/1988;