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Lucindo J Quintans-Júnior,
Daniela M Andrade,
Ana Helena Patrus,
Adriana G Guimarães,
Hugo C R Jesus, Clóvis R P Franco,
Dinalva B Queiroz,
Péricles B Alves,
Angelo R Antoniolli,
Eduardo G Azevedo,
Marco Antonio Botelho,
Leonard Edward Bannet
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ABSTRACT: The larvicidal activities of essential oils from the leaves of Artemisia vulgaris L., Cymbopogon flexuosus (Nees ex Steud.) Wats. and Piper tuberculatum Jacq. were evaluated using third-instar larvae of Aedes aegypti. The essential oils were obtained by hydrodistillation and analyzed by GC and GC-MS. The essential oil of P. tuberculqatum had the lowest LC50 value (106.3 +/- 2.2 microg/mL), followed by that of A. vulgaris (114.1 +/- 1.7 microg/mL) and C. flexuosus (121.6 +/- 0.8 micro/mL). The results show that these essential oils may be potent sources of natural larvicides.
Natural product communications 10/2012; 7(10):1391-2. · 1.24 Impact Factor
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ABSTRACT: To investigate the chemical composition and vasorelaxant effect of the essential oil of Lippia alba (EOLA) in rat mesenteric artery.
Chemical composition of EOLA was investigated by gas chromatography-mass spectrometry (GC/MS). Vasorelaxant effect was evaluated in vitro in rat superior mesenteric artery rings.
GC/MS analysis revealed the presence of 19 compounds, with geranial (48.58%) and neral (35.42%) being the major constituents. In intact rings precontracted with phenylephrine (Phe: 1 μM), EOLA (100-1000 μg/mL) induced relaxation, where the maximal effect (Emax) was 110.8 ± 10.8%. This effect was not modified after endothelium removal (Emax = 134.8 ± 16.5%), after tetraethylammonium (TEA) (Emax = 117.2 ± 4.96%), or in rings precontracted with KCl (80 mM) (Emax = 112.6 ± 6.70%). In addition, EOLA was able to inhibit the contraction caused by CaCl(2) and produced a small but significant (P<0.05) additional effect (from 70.5 ± 3.4 to 105.3 ± 13.5%, n = 5) on the maximal relaxation of nifedipine (NIF: 10 μM).
The results demonstrated that EOLA induces endothelium-independent vasorelaxation, which appears to be caused, at least in part, by blocking Ca(2+) influx through voltage-operated Ca(2+) channels.
Indian Journal of Pharmacology 11/2011; 43(6):694-8. · 0.27 Impact Factor
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Clóvis R P Franco,
Angelo R Antoniolli,
Adriana G Guimarães,
Daniela M Andrade,
Hugo C R Jesus, Péricles B Alves,
Leonard Edward Bannet,
Ana Helena Patrus,
Eduardo G Azevedo,
Dinalva B Queiroz,
Lucindo J Quintans-Júnior,
Marco Antonio Botelho
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ABSTRACT: The composition of three samples of essential oil (EO) extracted from the leaves and flowers of Hyptis fruticosa (Lamiaceae) were investigated by GC/MS and GC-FID. The variability of the constituents and biological activity were evaluated in the oil samples. Acetic acid-induced abdominal constrictions and formalin-induced pain tests in mice were used for screening the antinociceptive activity. The possible antagonism of the essential oils or morphine (MOR) antinociceptive effects by pretreatment with naloxone, showed no influence on the antinociceptive action of the oils in the acetic acid-induced writhing test. All examined oil samples presented antinociceptive activity. The oil sample obtained from the leaves collected during the vegetative growth stage, near São Cristóvão at Sítio Tujubeba exhibited the highest effect. The same oil sample had a main percentage of 1,8-cineole (18.70%). Nevertheless, the oil obtained from flowers collected at the same location, showed a significant difference (p < 0.05) in the response intensity in the first phase of paw licking (100 mg/kg) possibly due to the higher contents of α-pinene (20.51%) and β-pinene (13.64%). The results provide evidence for the use of H. fruticosa by traditional medicine practitioners in the management of pain.
Phytotherapy Research 03/2011; 25(11):1693-9. · 2.09 Impact Factor
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ABSTRACT: Hyptis pectinata Poit (Lamiaceae) is grown in the northeastern regions of Brazil and is popularly known as "sambacaitá" or "canudinho". It is extensively used in folk medicine to treat inflammatory conditions, bacterial infections, pain, and cancer.
Hyptis pectinata essential oil (EO, 10, 30, and 100mg/kg, p.o.) and the reference drugs morphine (5mg/kg, p.o.) and acetylsalicylic acid (ASA, 200mg/kg, p.o.) were evaluated using models for analgesia (acetic acid-induced contortions and hot plate) or inflammation (formalin-induced licking response and the subcutaneous air-pouch model). To elucidate the EO's mechanism of action, animals were pre-treated with the opioid receptor antagonist naloxone (1mg/kg, i.p.), the cholinergic antagonist atropine (1mg/kg, i.p.), or l-nitro arginine methyl ester (l-NAME, 3mg/kg, i.p.) 30 min prior to the oral administration of the EO.
The EO significantly inhibited the number of writhings and the time the animals spent licking their formalin-injected paws (second phase). The EO, at doses of 30 and 100mg/kg, increased baseline measurements and area under the curve measurements in the hot plate model, respectively. The administration of naloxone reversed the antinociceptive effect of the EO in the hot plate model. l-NAME significantly reversed the effects of the EO in the contortions and hot plate models. Atropine completely reversed the antinociceptive activity of the EO in all models. Additionally, the EO inhibited the inflammatory process induced by subcutaneous carrageenan injection by reducing cell migration, exudate volume, protein concentration, and inflammatory mediators (nitric oxide, prostaglandin E2, IL-6, and TNF-α) produced in the pouch.
Our results indicate that the Hyptis pectinata essential oil exhibits antinociceptive effects, likely mediated by opioid and cholinergic receptors, and anti-inflammatory activity through the inhibition of nitric oxide and PGE2 production.
Journal of ethnopharmacology 01/2011; 134(3):725-32. · 2.32 Impact Factor
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José Cláudio D Aguiar,
Gilvandete M P Santiago,
Patrícia L Lavor,
Helenicy N H Veras,
Yana S Ferreira,
Michele A A Lima,
Angela M C Arriaga,
Telma L G Lemos,
Jefferson Q Lima,
Hugo C R de Jesus, Péricles B Alves,
Raimundo Braz-Filho
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ABSTRACT: The chemical compositions of the essential oils from the peel of ripe and unripe fruits of Hymenaea courbaril L., obtained by hydrodistillation, were analyzed by GC and GC-MS. The main constituents of the essential oil from the peel of the ripe fruits were the sesquiterpenes alpha-copaene (11.1%), spathulenol (10.1%) and beta-selinene (8.2%), while germacrene-D (31.9%), beta-caryophyllene (27.1%) and bicyclogermacrene (6.5%) were the major compounds in the oil from unripe fruits. The essential oils were tested against Aedes aegypti larvae and showed LC50 values of 14.8 +/- 0.4 microg/mL and 28.4 +/- 0.3 microg/mL for the ripe and unripe fruit peel oils, respectively. From the peel of the ripe fruits, the diterpenes zanzibaric acid and isoozic acid were isolated, along with the sesquiterpene caryolane-1,9beta-diol. To the best of our knowledge, this is the first report of this sesquiterpene in the genus. The structures of all compounds isolated were identified on the basis of their spectral data (IR, MS, 1D- and 2D-NMR) and by comparison with literature spectral data.
Natural product communications 12/2010; 5(12):1977-80. · 1.24 Impact Factor
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ABSTRACT: The essential oils of Croton heliotropiifolius Kunth (Euphorbiaceae) and Croton pulegiodorus Baill. were selected for larvicidal evaluation against Aedes aegypti L. (Diptera: Culicidae) and studied qualitatively and quantitatively by GC and GC-MS. Sixty-one compounds representing 92.03% (C. heliotropiifolius) and 85.68% (C. pulegiodorus) of the essential oils, respectively, have been identified. The major components of C. heliotropiifolius essential oil were identified as beta-caryophyllene (35.82%), bicyclogermacrene (19.98%), and germacrene-D (11.85%). The major components in C. pulegiodorus essential oil were identified as beta-caryophyllene (20.96%), bicyclogermacrene (16.89%), germacrene-D (10.55%), tau-cadinol (4.56%), and beta-copaen-4-alpha-ol (4.35%). The essential oil of C. pulegiodorus (LC50 159 ppm) was more effective against Ae. aegypti than that of C. heliotropiifolius (LC50 544 ppm). In order to verify whether the major compound of both essential oils is the active principle responsible for the larvicidal activity, beta-caryophyllene was purchased and its larvicidal potential was further evaluated. However, beta-caryophyllene (LC50 1038 ppm) showed weak larvicidal potency. Results of larvicidal evaluation suggest the existence of a synergistic effect of minor components in the essential oils.
Pharmaceutical Biology 06/2010; 48(6):615-20. · 0.88 Impact Factor
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ABSTRACT: Ocimum basilicum (Lamiaceae) and other species of the same genus are used as medicines for the treatment of central nervous system (CNS) diseases. In this report, we have investigated the possible CNS depressant and anticonvulsant effects of Ocimum basilicum (access "Maria Bonita") leaf essential oil (EO) in different experimental models. GC-MS and GC-FID analysis of the essential oil resulted in the identification of 7 compounds constituting 98.8% of the total oil. 1.8-cineole, linalool, and geraniol were the principal components, comprising 92.9% of the oil. EO, at all doses, showed depressant CNS activity as revealed in the general pharmacological screening: decrease of spontaneous activity, ptosis, ataxia, and sedation. Additionally, all doses of EO induced a significant increase of sleeping time (p 0.05). Our data suggests that EO possesses CNS depressant and anticonvulsant properties which could be mediated by an interaction with central GABAergic receptors.
Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas. 01/2009;
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ABSTRACT: Ocimum selloi essential oil (2, 20, and 200 mg/kg; p.o.) reduced, in a dose-dependent way, the abdominal contraction induced by acetic acid (0.6%; i.p.) and the diarrhea episodes induced by castor oil in mice. At the higher dose (200 mg/kg; p.o.), the essential oil significantly reduced intestinal transit (P<0.05) in the charcoal meal test. The main component detected in O. selloi essential oil was methyl chavicol (98%; GC and GC/MS).These effects seems to support the use of O. selloi against diarrhea, intestinal spasm and visceral pain.
Fitoterapia 07/2008; 79(7-8):569-73. · 1.85 Impact Factor
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Igor A C Menezes,
Maxsuel S Marques,
Thiago C Santos,
Kellyane S Dias,
Aline B L Silva,
Iderjane C M Mello,
Ana C C D Lisboa, Péricles B Alves,
Sócrates C H Cavalcanti,
Rosilene M Marçal,
Angelo R Antoniolli
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ABSTRACT: The essential oil of the Hyptis fruticosa leaves was analyzed by GC/MS and evaluated for antinociceptive property as well as acute toxicity in mice. The essential oil, at doses of 100, 200, and 400 mg/kg (s.c.), produced significant inhibition of acetic acid-induced writhing, but did not manifest a significant effect in hot-plate test. There was no acute toxicity at doses up to 5 g/kg. Bicyclogermacrene, 1,8-cineole, alpha-pinene, and beta-caryophyllene were the major compounds detected in the essential oil.
Fitoterapia 05/2007; 78(3):192-5. · 1.85 Impact Factor
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Igor A.C. Menezes,
Maxsuel S. Marques,
Thiago C. Santos,
Kellyane S. Dias,
Aline B.L. Silva,
Iderjane C.M. Mello,
Ana C.C.D. Lisboa, Péricles B. Alves,
Sócrates C.H. Cavalcanti,
Rosilene M. Marçal,
Angelo R. Antoniolli
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ABSTRACT: The essential oil of the Hyptis fruticosa leaves was analyzed by GC/MS and evaluated for antinociceptive property as well as acute toxicity in mice. The essential oil, at doses of 100, 200, and 400 mg/kg (s.c.), produced significant inhibition of acetic acid-induced writhing, but did not manifest a significant effect in hot-plate test. There was no acute toxicity at doses up to 5 g/kg. Bicyclogermacrene, 1,8-cineole, α-pinene, and β-caryophyllene were the major compounds detected in the essential oil.
Fitoterapia.
