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Publications (2)5.61 Total impact

  • K S Vågnes, O Vågnes, V Bakken
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    ABSTRACT: Gingipains are potent virulence factors of Porphyromonas gingivalis and are likely to be associated with the development of periodontitis. It is, therefore, suggested that gingipain inhibition by vaccination could be a useful therapy for adult periodontitis. This study investigated the ability of antibodies raised against the catalytic part and the adhesin/haemagglutinin part of HRgpA to prevent haemagglutination and fibronectin degradation caused by P. gingivalis. We constructed two DNA vaccines, one containing the adhesin part of HRgpA and one with the catalytic part of HRgpA. BALB/c mice were immunized intramuscularly with either catalytic-part-encoding plasmids, adhesin-part-encoding plasmids or empty control plasmids. Sera from mice immunized with the catalytic vaccine or the adhesin vaccine each showed inhibition of human fibronectin degradation. A DNA vaccine encoding the adhesin or catalytic part of HRgpA induces responses that inhibit the degradation of molecules important for the structure and function of gingival and bone tissues.
    Oral Microbiology and Immunology 03/2007; 22(1):46-51. · 2.81 Impact Factor
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    ABSTRACT: The beta-adhesin part of the Porphyromonas gingivalis W50 (ATCC 53978) protease HRgpA was cloned in an eukaryotic expression vector and expressed in COS-7 cells. The monoclonal antibody MAb (61BG1.3), specific for the hemagglutinating domain of beta-adhesin, recognized the expressed beta-adhesin in the transfected cells both by immunoblot and immunofluorescence. Sprague Dawley rats were immunized intramuscularly with beta-adhesin encoding expression plasmid and expression plasmid without beta-adhesin insert. Skeletal muscle tissue at the site of immunization in the beta-adhesin immunized animals was shown to express this protein. The immunization induced a beta-adhesin-specific antibody response. Sera from the immunized animals were tested for hemagglutination inhibiting activity. Due to high natural inhibiting activity in all rat sera tested, no increased hemagglutination inhibition was detected in sera from the beta-adhesin immunized animals.
    Oral Microbiology and Immunology 05/2004; 19(2):77-82. · 2.81 Impact Factor