Nabila Degheidy

National Research Center, Egypt, Al Qāhirah, Al Qāhirah, Egypt

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Publications (6)2.09 Total impact

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    ABSTRACT: One hundred non pregnant female buffaloes (Bubalus bubalis), aged 4-8 years, were examined. Thirty healthy animals served as controls and 70 animals were diagnosed with fatty liver syndrome according to the clinical examination, liver ultrasonography, histopathological examination, blood contents and serum analysis. The clinical symptoms were anorexia, decrease in milk production, decrease in ruminal movement and rectal temperature. Moderate and severe hyperechogenicity of the hepatic parenchyma were clear in cases of moderate and severe fatty liver respectively after ultrasonographic examination. Post-mortem examination revealed enlarged pale yellow livers with rounded edges and presence of small droplets of fat. Microscopically, the hepatocytes showed diffuse fatty infiltration which had signet rings appearance due to the presence of intracellular droplets of triglycerides in liver cells. Blood picture showed a significant decrease in hemoglobin, RBCs and WBCs and significant increase in PCV (p<0.05). Serum analysis showed a significant decrease (p<0.05) in TP, albumin, ALT, TG and a significant increase (p<0.05) in GGT, globulin and AST. It was concluded that the ultrasonography, histopathology and clinical examination as well as blood picture and serum analysis support the diagnosis of fatty liver in non-pregnant buffaloes.
    01/2014; 12:682-686.
  • N. S. Degheidy, H. A. Shalaby
    Research Journal of Parasitology 02/2010; 5(2):79-89.
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    ABSTRACT: Fasciolosis is a world wide zoonotic disease of herbivorous animals. In Egypt, it had been considered to be the most chronic production limiting disease of ruminants. The current investigation was carried out on fifteen male baladi goats from Giza Governorate (about 12 months age) free from previous infection with F. gigantica. It was divided into 5 groups, the first group (G1) was vaccinated with unbound fraction proteins, the second group (G2) was vaccinated with natural glutathione-S-transfers (GST), the third group (G3) control group was vaccinated with Freund’s adjuvant, The fourth group (G4) kept as control positive and the fifth group (G5) kept as control negative. Blood samples were collected to assess the AST, ALT, ALP, Total protein, Serum albumin and Serum globulin. Liver samples were collected from slaughtered immunized and non-immunized goats to study the pathological changes. During vaccination period, no significant changes were detected for all groups regarding production of AST, ALT and ALP. After challenge, enzymes levels were higher in infected groups (G3 and G4) than vaccinated groups (G1 and G2). Histopathological changes in liver of GST immunized group were less obvious than other group that immunized with unbound fraction. There were variations in the biochemical parameters between infected and control groups. These variations were typified by considerably higher serum enzymes and total proteins level in the infected goats. Gross and microscopical liver damage were reduced in the immunized group compared to the control group.
    Journal of Genetic Engineering and Biotechnology. 01/2010; 8(2):7-16.
  • Proceedings of the 5th International Conference, Veterinary Research Division, National Research Center, Cairo, Egypt; 01/2010
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    ABSTRACT: The reason(s) why human antibodies raised against hepatitis C virus (HCV) E2 epitopes do not offer protection against multiple viral infections may be related to either genetic variations among viral strains particularly within the hypervariable region-1 (HVR-1), low titers of anti E2 antibodies or interference of non neutralizing antibodies with the function of neutralizing antibodies. This study was designed to assess the immunogenic properties of genetically conserved peptides derived from the C-terminal region of HVR-1 as potential therapeutic and/or prophylactic vaccines against HCV infection. Goats immunized with E2-conserved synthetic peptides termed p36 (a.a 430-446), p37(a.a 517-531) and p38 (a.a 412-419) generated high titers of anti-p36, anti-p37 and anti-P38 antibody responses of which only anti- p37 and anti- p38 were neutralizing to HCV particles in sera from patients infected predominantly with genotype 4a. On the other hand anti-p36 exhibited weak viral neutralization capacity on the same samples. Animals super-immunized with single epitopes generated 2 to 4.5 fold higher titers than similar antibodies produced in chronic HCV patients. Also the studied peptides elicited approximately 3 fold increase in cell proliferation of specific antibody-secreting peripheral blood mononuclear cells (PBMC) from immunized goats. These results indicate that, besides E1 derived peptide p35 (a.a 315-323) described previously by this laboratory, E2 conserved peptides p37 and p38 represent essential components of a candidate peptide vaccine against HCV infection.
    Virology Journal 02/2009; 6:66. · 2.09 Impact Factor
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    ABSTRACT: A polyvalent inactivated respiratory virus vaccine adjuvanted with Nigella sativa oil containing bovine viral diarrhea (BVD), Infectious Bovine Rhinotracheitis (IBR), Parainfluenza type 3 (PI-3) and bovine respiratory syncytial virus (BRSV) was evaluated in pregnant dams. Dams were at late stage of pregnancy (third trimester). The neutralizing antibody started to increase following vaccination at 3 weeks post vaccination above the protective level and increase to maximal level at parturition as follows for dams delivered after 2nd vaccination during 1-1.5 months were 1.70, 1.80, 1.90, and 1.65 for BVD, IBR, PI-3 and BRS viruses respectively. Dams delivered after 2nd vaccination during 2-3 months were 1.95, 2.00, 2.10, and 2.00 for BVD, IBR, PI-3 and BRS viruses respectively. The titers of the offspring born to vaccinated cows are as follows for calves delivered after 2nd vaccination during 1-1.5 months were 1.25, 0.80, 0.55, and 0.60 for BVD, IBR, PI-3 and BRS viruses respectively. Calves delivered after 2nd vaccination during 2-3 months were 1.68, 0.90, 0.43, and 0.74 for BVD, IBR, PI-3 and BRS viruses respectively. The Nigella Sativa oil adjuvanted vaccine can be used safely in immunization of pregnant dams to control the infection in newly borne calves.
    Bulletin of animal health and production in Africa. Bulletin des santé et production animales en Afrique 01/2007; 55(1):5-11.