N M Iudintseva

Publications (5)0 Total impact

• Article: [Reconstruction of the connective tissue as a result of transplantation of fibrins dermal equivalent to the wounds of experimental animals].

  N M Iudintseva, N M Pleskach, L V Smagina, M I Blinova, I A Samusenko, G P Pinaev
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  ABSTRACT: We analyzed the effectiveness of wound healing in rats after application of the dermal equivalent (DE) based on fibrin with dermal fibroblasts. Histological studies of newly formed dermis biopsy samples selected during its recovery in the model wound in laboratory animals have shown a positive effect of DE on wound healing. It was found a significant increase in the area of collagen fibers, in the number of prekapillaries, capillaries and postcapillaries in the granulation tissue after application of DE compared with the control group, suggesting a more intense repair.
  Tsitologiia 01/2010; 52(9):724-8.
• Article: [The cultivation of cells on the porous titanium implants with the different structure].

  M I Blinova, N M Iudintseva, N S Nikolaenko, I L Potokin, G Raykhtsaum, M Pitkin, G P Pinaev
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  ABSTRACT: The data on human dermal fibroblasts and rabbit mesenchymal stromal cells cultivation on porous titanium implants are presented in given paper. Two types of implants were used: type 1--with irregular pores formed by pressed titanium particles and type 2--with regular pores formed by coalescence of one-size titanium particles into implant. The goal of this study was to choose the type of titanium implant porosity which ensures the tightest interaction of titanium implant with surrounding tissue cells after implantation in the body. Cells were cultivated on implants for 7 days and in both cases they formed confluent monolayer on the implants surfaces. That indicated adhesion, migration and proliferation of cells on such implants. Condition of cells cultured on titanium implants was controlled by scanning electron microscopy. The character of fibroblasts interaction with given implants was different depending on porosity type of implants. On implants with irregular pores, the cells were more spread and overlapped the pores spreading over neighbored particles. On implants with regular pores that formed by one-size particles into implant, the fibroblasts covered these particles not overlapping the pores and seldom interacted with neighbored particles by small outgrowths. There was no tight interaction of particles into implant. In implants formed by pressed particles, the cells grew not only on the surface but also in the depth of implant. Thereby, we suppose that more tight interaction of cells with titanium implant and, supposedly, tissues with implant in an organism will take place in the case of implant structure formed by pressed titanium particles.
  Tsitologiia 01/2010; 52(10):835-43.
• Article: [Application of dermal fibroblasts in complex treatment of patients with trophic ulcers of venous etiology].

  A Zh Mel'tsova, V V Gritsenko, P I Orlovskiĭ, V V Tomson, V V Sabel'nikov, E K Shulepova, A i Prokopets, G P Pinaev, M I Blinova, N M Iudintseva
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  ABSTRACT: The authors have analyzed the results of an experimental-clinical investigation devoted to studying the efficiency of local treatment of trophic ulcers at the stage of healing using transplantation of dermal allogenic fibroblasts. The method was proved to accelerate epithelization and to shorten the time of preparing to operations for correction of the venous blood flow.
  Vestnik khirurgii imeni I. I. Grekova 02/2007; 166(1):72-7.
• Article: [Effect of melanins from black yeast fungi on cultured human cells. I. Proliferation of keratinocytes and fibroblasts].

  M I Blinova, N M Iudintseva, N V Kalmykova, E V Kuz'minykh, N A Iurlova, O A Ovchinnikova
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  ABSTRACT: Results of screening of the influence exerted by yeast black melanin on the proliferation of human skin keratinocytes and embryonic fibroblasts are presented. The optimal concentration of the investigated melanins was found to be within 0.005 and 0.0001 mg/ml. 17 samples of DHN-melanin from black yeast and 2 commercial samples of [symbol: see text]OPA-melanin (natural and synthetic) were investigated. It was established that keratinocyte proliferation was inhibited by 3 black yeast melanin samples; the influence of other 14 samples was the same as in the control. Keratinocyte proliferation was stimulated only by a commercial sample of natural [symbol: see text]OPA-melanin at concentration 0.005 mg/ml. The synthetic melanin at concentrations 0.005 and 0.001 mg/ml inhibited keratinocyte proliferation. Of the 17 investigated black yeast melanin samples, only one sample stimulated fibroblast proliferation at concentration 0.005 mg/ml. Three other samples inhibited the proliferation; of these one sample did it at all used concentrations, and two samples at concentration 0.0001 mg/ml. The rest 13 samples of black yeast DHN-melanins and the synthetic [symbol: see text]OPA-melanin did not differ in either action from the control.
  Tsitologiia 02/2002; 44(8):780-7.
• Article: [Effect of melanins from black yeast fungi on cultured human cells. II. Differentiation of keratinocytes in vitro].

  M I Blinova, N V Kalmykova, N M Iudintseva, E V Kuz'minykh, N A Iurlova, O A Ovchinnikova, I L Potokin
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  ABSTRACT: Data on the influence of the black yeast melanin (3 samples) on the in vitro differentiation of human keratinocytes are presented. The effect of melanins was estimated by the morphological state of keratinocytes using electron microscopy. The obtained differences in the state of the formed multilayer keratinocyte sheets depended on the melanin sample.
  Tsitologiia 02/2002; 44(8):788-91.