MinQ Wei

Fudan University, Shanghai, Shanghai Shi, China

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Publications (2)0 Total impact

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    ABSTRACT: To investigate changes in biologic properties of non-small-cell lung cancer (NSCLC) A549 cells whose EGF receptor (EGFR) expression was suppressed by short interference RNA (siRNA). A549 cells were transfected with synthetic EGFR sequence-specific siRNA by Lipofectamine. EGFR expression was examined by Western blot and flow cytometry. The biological features of the transfected A549 cells were assessed by cell cycle analysis, colony formation and chemosensitivity assay. Sequence-specific siRNAs targeting EGFR significantly down-regulated its expression in A549 cells. Cell growth and colony formation were inhibited by 85.0% and 63.3%, respectively, as compared to the non-sequence-specific siRNA treated cells. Decreased EGFR expression was accompanied by 12.7% increase in A549 cells in G(0)-G(1) phase and 6.6% decrease in S-phase. The EGFR sequence-specific siRNA transfected A549 cells were much more sensitive to the cytotoxic effect of cisplatin with a 77.2% decrease in IC(50) compared to the non-sequence-specific iRNA transfected A540 cells. Down regulation of EGFR expression of NSCLC by sequence-specific siRNA may be considered as an additional option in the treatment of EGFR over-expressing cancers, including NSCLC.
    Zhonghua zhong liu za zhi [Chinese journal of oncology] 01/2005; 26(12):713-7.
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    ABSTRACT: To investigate whether RNA interference (RNAi) induced by small interference RNA (siRNA) could suppress epidermal growth factor receptor (EGFR) expression in non-small-cell lung carcinoma (NSCLC) cells. SPC-A-1 cells were transfected using chemically synthesized double stranded RNA (dsRNA) formulated with Lipofectamine 2000. The EGFR numbers were determined by both Western blot and flow cytometry. The antiproliferative effects of dsRNA-EGFR were assessed using cell counts and colony assay. Cell cycle analysis was carried out via flow cytometry. The chemosensitivity of transfected cells to cisplatin was determined by MTT. Sequence specific siRNAs targeting EGFR down-regulated EGFR expression significantly. Compared with the control group, dsRNA-EGFR reduced the cell numbers by 78.3% and decreased the colonies by 66.8%. Cell cycle analysis showed that dsRNA-EGFR induced accumulation of cells in G0-G1 phase by 17.48% with a significant decrease in the percentage of cells in S-phase by 19.20% relative to the control. Based on the value of IC50 obtained by Origin 6.0 software, we concluded that dsRNA-EGFR increased the sensitivity of SPC-A-1 to cisplatin by seven-fold. Sequence specific siRNAs targeting EGFR was capable of suppressing EGFR expression, and therefore, significantly inhibiting cellular proliferation and inducing cell cycle arrest. The finding from chemosensitivity assay further revealed that dsRNA-EGFR was associated with an addictive or synergistic effect on tumor growth inhibition when combined with cisplatin. The successful application of dsRNA-EGFR for inhibition of proliferation in EGFR overexpressing cells extends the list of available therapeutic modalities in the treatment of human cancer.
    Zhonghua nei ke za zhi [Chinese journal of internal medicine] 06/2004; 43(5):345-8.